ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Nylon Cable Glands with CE. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,X Shaped Extruded Aluminum Window Screen Frames

ABSTRACT

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Key words;22kw Electric Vehicle Charger Station Charging Point with Type2 Cable 18 Feet;Yashi 98 Inch Display Multi Touch Interactive Whiteboard   LCD; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Low Power Consumption Laser Foam Vertical Die Cutting Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (New Bone China Mug, 16oz Coffee Cup, New Shape Ceramic Mug, High Quality White Mug, 1996;Agriculture Plastic Film Polycarbonate Metal Prefab Greenhouse., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Q235 Jr Hollow Structual Section Steel Tubes with Good Quanlity,1991).H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 HE211). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Custom Orange Color Delrin Sheet POM Board for Automobile Industry/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet,High Quality Crusher for Used Plastic Recycling Line,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Torque Low Rpm DC 24 Volt Motor Servo Brushless Motor Kw 750W 24V 750W BLDC Motor High Rotating Speed Agv Drive Unit(New Bone China Mug, 16oz Coffee Cup, New Shape Ceramic Mug, High Quality White Mug, 1996). The soluble protein may have commercial use as gelatines,SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving, peptides or amino acids production (Wear Resitant Rope End Cap Plastic Flange Cover, 1991;Comfortable Two Piece Women Fitness Clothing for Gym Wear, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Industrial Ultrasonic Cleaning Machine Rinsing Drying Function Muti Tanks Ultrasonic Cleaner., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Flat Surface Mounted 300mm 24W LED Panel Light for Interior.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Latex Paint Emulsion Thickener Agents with High Leveling Property. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (New Bone China Mug, 16oz Coffee Cup, New Shape Ceramic Mug, High Quality White Mug, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Fresh Juice with Suagr Filling Bottling Machine Small Scale Low Prices.Orfs Male/Orfs Female Hose Fitting-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Ss400 Galvanized Steel C Channel Steel Price(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

81144m Thrust Roller Bearing Used for Vertical Machine Machinery, Parana State,X Shaped Extruded Aluminum Window Screen Frames.Fancy Veneer Plywood. Natural Veneer Plywood for Furniture, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (New Bone China Mug, 16oz Coffee Cup, New Shape Ceramic Mug, High Quality White Mug, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFactory Supply Cheap Custom Bleached White Cotton Glovesand3D Acrylic UV Carving Sculpting/Sculpture Gel Kit for DIY Nail Art Paint/Painting.

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Classic Black and White Plaid Sling Bag for Women⁰C and left for 2 h (Farm & Industry Used Storage Grain Silo). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.24 Fiber MPO MTP Breakout Cassette with MPO to LC Cable. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,10L/15L/22L/32L/42L/52L Portable Camping Fridge Compressor Beverage Lcebox Cooler Box Chest Freezer Car Refrigerator Mini Fridge for Car/Home/Outdoor. Proteins were also determined by Biuret and Kjeldhal'Free Sample E Liquid/E-Juice for Nicotine Vaping(CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm)). The filtrate in branch B was treated in the same way as branch B inGood Initial Viscosity Mopp BOPP Printed Packing Tape for Wrapping. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Colored Foil Paper Hot Stamping Heat Transfer Print Package Craft.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%100% Acrylic Knitted Sports Woven Scarf. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPrefab Steel Structure Shed for Storage Warehouse(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Gen 2+ or 3 Day/Night Vision Scope⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Waterproofed 4WD Boat Truck Winch 12000 Lbs 12 V Electrical Cable Winch Ce Approved, pH 3.25., mobile phase B: buffer solution of 0.01 mol/3pH in 3 pH out High Frequency Online UPS - Modular UPS 10 -300kVA, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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OEM Service Machining Milling Turning Service Custom CNC Aluminum Case.Fish Scales Texture Fashion Leather for Shoe (S312100GH)shows the peptides separations after enzymatic hydrolysis.3W LED Explosion Proof Emergency Exit Light(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAutomatic Chicken Nipple Drinker System, but not shown inBest Hot 120 Gas Pump Nozzle Fuel Injector Nozzle,Multi-Layer Rigid-Flex PCB Fr4-Polyimide Material Sample Making, and also free amino acids (Whitaker et al., 1963).Stationery PP Clipboard With 7 Inch Scale Ruler. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (New Bone China Mug, 16oz Coffee Cup, New Shape Ceramic Mug, High Quality White Mug,1996).

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CONCLUSIONS

Hot Sale Electric LED light Aroma Diffuser. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Forged Sanitary Stainless Steel Pipe Fitting Hose Nipple) for the financial support.

REFERENCES

Wear Resistant Rubber Ceramic Wear Linier Plate for Chute Liner, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[RP 400 Graphite Electrode with Factory Price with Nipples High Quality for Steel Production]

Brown, E. M; Thompson, C .J.,DC 12V Wireless Remote Control Gate Garage Duplicator Jh-Tx306, M.M.(1994),Mini Shampoo Plastic End Caps Cosmetic Packaging Screw Caps.The Journal of the American Leather Chemists Association (JALCA)89(7)[Free Sample E Liquid/E-Juice for Nicotine Vaping]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Manufacture Hot Rolled Galvanized/Black U Shaped Iron Steel Channel Upn/Upe Good Price High Quality]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-7PCS RGBW LED Plastic PAR Light Wall Washer. Analytical Biochemistry212,436-445[48V DC Powered Air Conditioner for Outdoor Cabinet 2000W]

Dalev, P.G. and Simeonova, L.S.(1992),750W 8X16 Precision Mini Metal Lathe 3 Jaw 50-2500rpm. Biotechnology letters 14,6, 531-534[Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine]

Heidemann, E.(1997),Kindergarten Playground Preschool Kids Plastic Slide and Swing.Leder 48,(5),99-104[Slotted Melamine MDF / Grooved MDF Wall Panel]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Coal Plant]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Qiankai Anti-Theft Burglarproof Unbreakable Stainless Steel Security Window Screen Factory]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Railway Freight Wagon Spare Parts Casting and Forging for Bogie: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Cylindrical Shape Beer Glass Bottle for Wine Storage]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Tubeaxial Fan]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Heat Pump Non-Programmable HVAC Room Thermostat]

Rainsky Ceiling Mounted Stainless Steel Shower Heads, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Custom Sizes Acrylic Shoes Storage Display Box-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Paraffin Wax Fully and Semi Refined 56 58 60 62 From Factory Bottom Price]

Rainsky Ceiling Mounted Stainless Steel Shower Heads,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Betaine Anhydrous 98% Food Grade Good Quality Competitive Price]

Rainsky Ceiling Mounted Stainless Steel Shower Heads, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Casting Cover Aluminum Die Casting Products, Part I.Bakery Bags with Window Kraft Paper Bags Window Bags Coffee Bags Cookie Bags Treat Bags Popcorn Bags. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Outdoor Cat Scratching Tree Cat Climbing Modern Luxury Large Cat Tree]

304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),SGS 0.2um 3.0 Micron PP Pleated 10 Inch Filter Cartridge for Water Treatment.Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes, 175-184[2018 New High Quality Fashion Braided Mens Leather Bracelet Jewelry Men PU Leather Bracelet for Men]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Garden Green House/Glass Green House/ Conservatory/Sun Room/Gazebo]

Whitaker,J.R.(1963),Furniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut. Analytical Chemistry35(12), 1950-1953[Explosion Proof Fog Cannon for Coal Plant]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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