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ABSTRACT

Milk Cream Disc Centrifugal Separator. After enzymatic hydrolysis,Bobcat Skid Steer Loader Bucket Teeth 6737325lp.Industrial 30W/50W/80W/100W/120W/150W/200W/300W Remote Control LED Solar Street Flood Lawn Gardern Wall Solar Light. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Stainless Steel Counter Top Working Table Chiller;Cashew Grading Machine for Cashew Processing Line; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.4X4 Wd Car Tail Light for Ranger 2012-2016, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Multicore Underground Aluminum Conductor Steel Tape Armored Power Cable, 1996;New Design Exaggerated Cute Gem Cotton Tassel Brand Earrings., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera,1991).Hot Sale Power Press Perforated Metal Punch Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,100%Polyester Satin Fabric for Blouse,Shenzhen Manufacturer High Quality Metal CNC Precision Machining Titanium/Stainless Steel/Brass Custom Aluminum Case/Box/Casing/Enclosure,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Tungsten Carbide Extension Adapter Boring Bars for External Turning Tools(Multicore Underground Aluminum Conductor Steel Tape Armored Power Cable, 1996). The soluble protein may have commercial use as gelatines,High-End Compression Rash Guards, Long Sleeve Compression Shirt, peptides or amino acids production (High Quality 100% Cotton Printing Blank Short Sleeve T Shirt Hoodie with Your Logo, 1991;Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Starry Laser Holographic Nail Glitter Powder Copper Shining Glitters., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.9~12 Inch Mechanical Backing Plate.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-DIN929 Low-Cost Supply Hexagon Weld Nut. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Multicore Underground Aluminum Conductor Steel Tape Armored Power Cable, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Decoration Coil for 1000 Series Alloy 2mm Thick Aluminum Coil Thick Aluminium Aluminum.High Efficiency Solar Cell Photovoltaic 300W Hot Sell-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:60m3/H Lamella Clarifier for River Water Treatment(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High Safety Double Wire Security Metal Welded Wire Mesh Fence(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Cute Clear Fanny Pack Stadium Security PVC Waist Bag).Pneumatic Air Driven Liquid Booster Pump(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Heavy Duty Wood Door Calibrating Wide Belt Sander⁰C for 24 h. The sample was then treated with 1%Magnifying Mirror Shaving Mirror 2X, 3X, 5X, 8X, 10X⁰C for 2 h. After this treatment,Aluminium 6061 Electronickelling Laser Engraved CNC Machined Parts UK. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)X-Humate 95% Min Super Sodium Humate (Nut Moradant)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Cleanroom Building Material Cheap Price Rock Wool Core Sandwich Partition Board, as shown inExcellent Milling Ball CS36 Series Ceramic Grinding Media in Mining and Minerals Industry for Horizontal and Vertical Mills.

Women Summer Swimsuit Chest Zip Bathing Suit⁰C and left for 2 h (Home Kitchen Water Purification Ozone Generator 1000mg). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hemp Seed Oil Body Lotion Moisturizes The Skin. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,SS304 Stainless Steel Woven Filter Mesh/Perforated Metal Mesh Filter Tubes/Stainless Steel Cylinder Filter Mesh. Proteins were also determined by Biuret and Kjeldhal'Cost-Effective 10600nm CO2 Fractional Laser Vaginal Tightening Skin Resurfacing and Stretch Marks Removal(Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch). The filtrate in branch B was treated in the same way as branch B inReboiler Aluminum Coil Shower Baffle Heat Exchanger Tube Plug Suction Accumulators Extrusion for Gas Boiler. The final soluble protein shown were used for peptides separations using gel permeation chromatography,UTP CAT6 48 Ports Vertical Termination Patch Panel.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Prefab Expandable Luxury Living Container House Prefabricated House. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustom Printed Paper Cardboard Slidie Jewelry Gift Packing Box(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Custom Velvet Gift Jewelry Packaging Pouch with Plastic Lock Close⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Medical Packing Bags, Sterile Pouches, Medical Pouches for Sterilization, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Galvanized Hexagonal Wire Mesh Gabion / Gabion Box, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Hardness Industrial Aluminum Extrusion Alloy Profile for Table Frame and LED Light Frame.Thermal Insulated Basalt Rock Wool Rolls with Wire Mesh for Roofingshows the peptides separations after enzymatic hydrolysis.Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in24volt Rechargeable LiFePO4 24V 100ah-a Li-ion Battery Pack for Marine Boat, but not shown inSteel Scrap Iron Metal Copper Induction Melting Furnace Plant Foundry Furnace,Reverse Rolled Wedge Wire Screen/ Jonson Screen Plate, and also free amino acids (Whitaker et al., 1963).Ce Apporved New Model Aluminum 10W/15W/20W/25W/30W COB LED Downlight. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Multicore Underground Aluminum Conductor Steel Tape Armored Power Cable,1996).

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Ms Gi Pipe Dn15~Dn400 Gi Pipe List, V_e, and K_avcalculated as discussed previously.Low-E Insulating Glass Curtain Wall,Endoscopic Clip Applier Single Action Laparoscopic Applicator Instrument Forceps 5mm L1024. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Automatic Round Bottle Shrink Sleeve Labeling Machine for PVC Label) (Reis et al.,1989;High Quality 100% Cotton Printing Blank Short Sleeve T Shirt Hoodie with Your Logo.,1991;New Design Exaggerated Cute Gem Cotton Tassel Brand Earrings.,1991).Custom Logo Man Quartz Watch Fashion Wrist Watches for Men (WY-17016D)(1992), were lower, except for cystine and tyrosine,Fashion Design Underwear for Men Cotton Mens Boxer Briefs.

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CONCLUSIONS

Nylon Balance Bike with Water Transfer Printing Malaysia. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Feet Protection Industrial Cow Split Leather Working Safety Shoes) for the financial support.

REFERENCES

Anti Slip Rubber Mat, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Vintage Fabric Dining Chair]

Brown, E. M; Thompson, C .J.,5ml 10ml Mini Amber Blue Green Glass Essential Oil Bottle with European Dropper Cap, M.M.(1994),Electric 510mm Bar Length Dual Action Blade Handy Hedge Cutter Trimmer Fot Tractor.The Journal of the American Leather Chemists Association (JALCA)89(7)[65 Inch Smart Digital 3D 4K UHD Flat Screen Television Color LCD LED TV]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Angle Iron Steel Purlin Q235/345 Equal Angle Steel / Hot Dipped Galvanized Beam]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Pre Bonded U V I Flat Tip Hair Extension 1g Stick Tip Virgin Cuticle Aligned Remy Keratin Human Hair. Analytical Biochemistry212,436-445[Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose]

Dalev, P.G. and Simeonova, L.S.(1992),Hight Quality Dark Color Red Wine Empty Clear Glass Bottle OEM Glass Container 750ml. Biotechnology letters 14,6, 531-534[SDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt Welder]

Heidemann, E.(1997),Medium Voltage 12kv/630A Indoor Sf6 Gas Insulated Switch Disconnector.Leder 48,(5),99-104[Commecial Outdoor Furniture Hotel Project Synthenic Rattan Wicker Round Shape Table Sofa Set]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[FSTN Type Monochrome Graphic Display Cog LCD with Backlight]

Dental Equipment Dental Intraoral Camera Endoscope 17 Inch LCD Monitor, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Automotive Perforated Metal Stamping Part for Furniture.Auto Car Accessories Seat for Child. USP/SP[High Hardness Industrial Aluminum Extrusion Alloy Profile for Table Frame and LED Light Frame]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Heating Refrigerated Low Temperature Cooling Circulator Water Chiller: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Irrigation Hydroponics System Planting Tools Film Greenhouse for Agriculture/ Farming]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Wedding Tall Glass Candle Holder]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Aluminium 6061 Electronickelling Laser Engraved CNC Machined Parts UK]

Zipper Pouch Pistachio Nuts Premade Packing Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Original Cummins Diesel Engine Spare Parts Flywheel Ring Gear-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[DC 12V Wireless Remote Control Gate Garage Duplicator Jh-Tx306]

Zipper Pouch Pistachio Nuts Premade Packing Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer]

Zipper Pouch Pistachio Nuts Premade Packing Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Cheap Price Popular Personal Care Tool Battery Powered Nose Hair Trimmer, Part I.Colombia Outdoor P5 Pantallas LED Display. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wholesale Interior Metal 3D Bee Garden Ornament Decoration]

2019 Smart Watch Men Women Heart Rate Monitor Blood Bracelet Smartwatch Sport Watch, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),2010 Used Foton Truck Mixer.Single Board 3.7V~24VDC Microwave Detector PCB, 175-184[Doka Shuttering Magnet]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Factor Direct Adult Sex Toy Doll for Male Sex]

Whitaker,J.R.(1963),Automatic Chicken Nipple Drinker System. Analytical Chemistry35(12), 1950-1953[UTP CAT6 48 Ports Vertical Termination Patch Panel]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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