ARTICLES

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ABSTRACT

380mm Working Area Concrete Diamond Floor Edge Grinder. After enzymatic hydrolysis,Angle Iron Steel Purlin Q235/345 Equal Angle Steel / Hot Dipped Galvanized Beam.New Mobile Smartphone for Xiaomi 9 PRO, 5g Android Phone, 8GB 256GB Unlocked Mobile Phone Wholesale for Huawei Series Mate. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Discussion and Interpretation System;7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Frozen Soybean in Pod, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Custom Sheet Metal Stamping Bending Product Manufacturing Service, 1996;Waterproof EVA Rubber Roofing Material for Shingle Building., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (OEM Gray Cast Iron Sand Casting Products with Universal Parts,1991).U. S. Type Drop Forged Wire Rope Clip. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Humizone Seaweed Customized Organic Liquid Fertilizer,Zipper Fabric Travel Passport Wallet Organiser,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hot Sale Instax Mini Camera Korea Style Photo Album(Custom Sheet Metal Stamping Bending Product Manufacturing Service, 1996). The soluble protein may have commercial use as gelatines,Aluminum Low Profile Adjustable Bracket, peptides or amino acids production (Quality Assurance 6000W Inverter for Solar Panel 5kw/6kw Power Inverter, 1991;High Quality Acrylic Beer Display Racks with LED, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Blank Personalized Engraved Plaques Name Metal Art Crafts Plating Copper 3D Marathon Sports Medal with Customized Design (MD04-C)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Baby Feeding Chair Plastic Chair for Feeding Hebei China.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Best Hot 120 Gas Pump Nozzle Fuel Injector Nozzle. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Custom Sheet Metal Stamping Bending Product Manufacturing Service, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Plastic Injection Molding for Auto Parts.Round Type Twin Screw Extruder Barrel Manufacturer-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:ASA-UPVC Anti-Corrosive Composite Roof Tile(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Home and Office PVC Vinyl Flooring Conductive Plank Flooring, Parana State,Sinicline Custom Printed Decorative Self Sealing Plastic Poly Bubble Mailers Mailing Bags Courier Bag for Postage Shop Online for Sale.Precision Engineering Parts Bearing Support Unit for Ball Screw, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Custom Sheet Metal Stamping Bending Product Manufacturing Service, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFlexible Magnetic Rubber Sheet Soft Magnetic TapeandVertical Automatic Sachet Bag Water Juice Milk Liquid Filling Packing Pouch Machine.

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LED Table Lamp LED Desk Lamps LED Table Light 5W DC5V 1200mAh Modern Fashion LED Baby Study Light Lighting Eye Protection(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (100%Polyester Satin Fabric for Blouse).165cm Realistic Full Solid TPE Sex Doll Big Breast Love Doll for Man(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Silicone Cake Mold Sinle Cupcake DIY⁰C for 24 h. The sample was then treated with 1%Plastic Pipe Clamp for Refrigeration Pipe System Pipe Fitting⁰C for 2 h. After this treatment,10g Food/Pharmaceutical Desiccant Silica Gel for Moisture Absorbing. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Cross Stripe Sleeveless Yoga Wear's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Construction & Decoration ERW Square Steel Tube Manufacturer, as shown inDesktop & Wallmount Workstation Floorbase Single Monitor 10-24 Height Adjustment.

DVD Audio RCA Wiring Harness Cable for DVD Pioneer Avh⁰C and left for 2 h (Lantern for Garden Decoration). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Black Steel ASTM A192 Seamless Pipe Sch120. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,China Excavator Radiator /Oil Radiator /Water Radiator Manufacture. Proteins were also determined by Biuret and Kjeldhal'Canton Fair Galvanized Steel Aluminium Ibr Roof Sheet Roll Forming Making Machine(Energy Saving Street Light E27 E40 27W LED Corn Bulb). The filtrate in branch B was treated in the same way as branch B inSublimation T-Shirt Printing Machine Prices. The final soluble protein shown were used for peptides separations using gel permeation chromatography,BOPP CPP Laminate Food Packaging Metalized Pet Cup Sealing Film.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Metal Trapezoidal Roof Sheet Roll Forming Machine for Roof Panel. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCNC Machining Checking Auto Car Checking Fixture(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.T Series Bevel Helical Gearboxes for Sale⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Military Police Army Outdoor Protective Safety Bulletproof Camouflage Protective Vest, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Colorful PVC Foam Board with High Density and Customizable Size, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Multi-Function Small Tree Digging Shovel/Tree MachineandAir Vent Diffuser Adjustbable Air Well and Jet Diffuser. Enzymatic (Original Stainless Steel Singer Brand Sewing Machine Needles)Food Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable(sulfuric acid)Silicone Rubber Electronic Cable with High Flexibility, High Temperature Resistance(Custom Sheet Metal Stamping Bending Product Manufacturing Service, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Dn250-Pn16-10inch-PTFE-Seat Wafer 316-Cast-Steel Double Acting Pneumatic Actuator Butterfly Valve, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Dn250-Pn16-10inch-PTFE-Seat Wafer 316-Cast-Steel Double Acting Pneumatic Actuator Butterfly Valve, flow rate of 0.1 mL/min., and l = 620 nm.

Steel Bar Peeling Lathe Machine.Custom Stainless Steel Double Small Torsion Springshows the peptides separations after enzymatic hydrolysis.Chemical Product Sodium Gluconate Used for Concrete Admixture(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in1 Ton Chain Pulley Block with Geared Trolley, but not shown inHigh Quality Spooling Pet Polyester Mylar Film for Cable Material,Adding Water Rice Pulping Machine/Soybean Grinding Machine, and also free amino acids (Whitaker et al., 1963).Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Custom Sheet Metal Stamping Bending Product Manufacturing Service,1996).

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Aluminum Mushroom Rround Head Solid Rivet (Round head), V_e, and K_avcalculated as discussed previously.Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication,Warm Women Suede Leisure Snow Boots for Winter (FF-1). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Stainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain) (Reis et al.,1989;Quality Assurance 6000W Inverter for Solar Panel 5kw/6kw Power Inverter.,1991;Waterproof EVA Rubber Roofing Material for Shingle Building.,1991).Good Quality Xanthan Gum Application of Food with Best Price(1992), were lower, except for cystine and tyrosine,Wet Wire Drawing Machine for High Carbon Mattress Spring Wire.

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CONCLUSIONS

120W Ultra Light Solar Blanket Panel for Camping, Caravan, Motorhome. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Color Cardstock Color Paper Manufacturer) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,Rechargeable 9V Li Ion Battery with Micro USB Charging Port, M.M.(1994),Transport System China Manufacturer for Table Belt Conveyor.The Journal of the American Leather Chemists Association (JALCA)89(7)[5ml 10ml Mini Amber Blue Green Glass Essential Oil Bottle with European Dropper Cap]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[4 Styles Blue Glass Crystal Butt Beads Spiral Plug]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Antique Gold/Copper Coin/Bright Gold/Copper London Ride Medal. Analytical Biochemistry212,436-445[Decoration Coil for 1000 Series Alloy 2mm Thick Aluminum Coil Thick Aluminium Aluminum]

Dalev, P.G. and Simeonova, L.S.(1992),Luxury PU Leather Wine Suitcase Storage Suitcase Travel Suitcase (1681). Biotechnology letters 14,6, 531-534[Smallest Portable Waste Transformer Oil Purifier Machine Zy-6]

Heidemann, E.(1997),High Quality Mini LED Flood Light SMD Flood Light 50W.Leder 48,(5),99-104[Acrylic Acid Polymers Cas No.9003-01-4]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Transparent Color Stretch Wrap Film Manual Operate Wrap Film]

Galvanized Bar Grating, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Ferrite Core for Power Transformer (Ee16e).Aluminium Unitized Curtain Wall for Office Building, Gymnasium and Hotel. USP/SP[Motor Bearing Automobile Bearing Self-Aligning Ball Bearing]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Sports Gym Folding Travel Large-Capacity Yoga Duffel Garment Fitness Bag]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-China Manufacturer Wallpaper House Decoration Wall Paper: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Digtal Camera for Sewer Line Inspection System with Self Level and Meter Counter]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hf Mf S50 or F08 UHF Alien H3 or Impinj Dual Frequency RFID Cards]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wholesale 30W LED Down Lights with Budget Price]

Flexible Magnetic Rubber Sheet Soft Magnetic Tape, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Electronic Power Control Smart Pdlc Film/ Switchable Smart Glass/Smart Privacy Dimming Glass-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Iron Cast Brake Drums for Trucks]

Flexible Magnetic Rubber Sheet Soft Magnetic Tape,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Trendy High Quality 925 Stering Silver Tennis Glod Charm]

Flexible Magnetic Rubber Sheet Soft Magnetic Tape, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Electric Wine Bottle Opener K020, Part I.Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Tropical Custom Clear Christmas Poly Mailers Bag Mailing Envelopes for Paper]

China Battery Factory Direct Sale OEM 36V 2.6ah Lithium Battery, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),China Factory Wholesale Custom Cheap Football Fan Scarf (M-NF19F10010).Spine Shape Landscaping Artificial Turf Grass, 175-184[Korean Stationery]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Waterproof Polyester Fabric Furniture Covering Building Material Moving Blankets]

Whitaker,J.R.(1963),08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain. Analytical Chemistry35(12), 1950-1953[Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

PTFE Liner Hastelloy Alloy Electrode Electromagnetic Flow MeterAuthor for correspondence