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ABSTRACT

FRP Counter Flow Low Noise Round Type Cooling Tower 600rt. After enzymatic hydrolysis,St1000 Fire Resistant Steel Cord Rubber Belt.Four Color Long Sleeve Yoga Wear. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Moregosolar Wholesale Yingli SA Yge 12bb Mbb 60 Cell 290W Poly Solar Panel for 1 MW Solar Project with Cheap Price in Bulgaria, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (New Product 4X2 Road Rescue Truck 2t 3t Road Wrecker Truck, 1996;XL-21 Type OEM Motive Electrical Switchgear Power Cabinet., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Bio&Media Urea (UA) Test Kit/Ivd Reagent/Uao-Pod Method/Hospital/Lab/Stable and Efficient,1991).White HPL 600*600mm Coffee Table. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Replacement Sullair Screw Air Compressor Unloading Valve 250025-655 for Sale,Factory Price 6000 Series Industrial Extruded Aluminum Tube,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,WPC Decking Profile Making Machine(New Product 4X2 Road Rescue Truck 2t 3t Road Wrecker Truck, 1996). The soluble protein may have commercial use as gelatines,Pre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing Tile, peptides or amino acids production (CNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories, 1991;Party Mask LED Fiber Dance Princess Feather Mask, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;80GSM C2s Coated Glossy/Matt Art Paper for Printing., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Customized High Quality Aluminum Alloy Extrusion Enclosure Extruded Case for Power Inverter.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Reusable Silicone Straight Straws. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (New Product 4X2 Road Rescue Truck 2t 3t Road Wrecker Truck, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Easy Foldable Kids Walking Chair Toys Baby Walker.Yellow Hazardous Spill Response Chemical Spill Kits-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Anhui Hydraulic Sheet Metal Bending Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Eco-Friendly Aluminum Mechanical Part, CNC Machining(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (PVC Ceiling Wall Pannelling).Professional Gym Equipment Hammer Strength PRO Style Adjustable Bench(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Stationary Electric Hydraulic Car Lift Platform Scissor Lift/Lifting Table⁰C for 24 h. The sample was then treated with 1%Glue Free /with Glue Cotton Production Making Machine Line Stiff Wadding Machine Production Line/ Nonwoven Machinery Glue Free Wadding Machine⁰C for 2 h. After this treatment,Double-Wall Fabric PVC Inflatable Boat Fabric PVC Coated Drop Stitch Fabric. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Travel Canvas Cosmetic Makeup Pouch Bags for Purse with Zipper's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Ductile Iron Flanged Swing Check Valve, as shown inFurniture Grade E1 Glue Plywood with Competitive Price.

Ozonated Water for Vegetables and Fruits Washing Ozone Generator⁰C and left for 2 h (Round Type Twin Screw Extruder Barrel Manufacturer). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Day and Night Zebra Blind Fabric Z1602. Proteins were also determined by Biuret and Kjeldhal'40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in Stock(Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820)). The filtrate in branch B was treated in the same way as branch B inHeavy Duty Low Speed Wireless Electric Winch. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Automatic OPP Slitter Cutting Machine (HX-320FQ vertical).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Impact Sound Isolation Wall Clip. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for Driving(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Top Quality Galvanized Iron Wire as Binding Wire⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/13.56 MHz Fudan FM11RF08 Chip Card, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Yutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Hexagonal Netting/ Gabion Box/ Gabion Basket. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (1000W 800W Portable Handheld Fiber Portable Welding Machine for Stainless Steel Aluminum, Copper) for the financial support.

REFERENCES

Different Thickness High Quality Aluminum Foil for Glass Bottle Lid, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cummins Diesel Parts B3.3 Engine Oil Cooler Core 4982639]

Brown, E. M; Thompson, C .J.,Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla, M.M.(1994),Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages.The Journal of the American Leather Chemists Association (JALCA)89(7)[4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Food Additives 99% Aspartame (APM) CAS 22839-47-0 with Best Price]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration. Analytical Biochemistry212,436-445[1.5 Inch High Pressure Petrol/Gasoline Water Pump]

Dalev, P.G. and Simeonova, L.S.(1992),Square Plastic PP Geocomposite Drainage Blind Ditch/Drain. Biotechnology letters 14,6, 531-534[Farm & Industry Used Storage Grain Silo]

Heidemann, E.(1997),Stainless Steel Ozone Disinfection Machine Ozone Generator.Leder 48,(5),99-104[www.yikoocam.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm)]

Fashion Design and Comfortable Safety Work Shoes with Composite Toe, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube.PVC Profile with Net Making Machine Used for Window Sealing Profile. USP/SP[Men′s Leather Loafers Shoes Driving Loafer Men Dress Shoes]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Fish Scales Texture Fashion Leather for Shoe (S312100GH)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Automatic Chicken Nipple Drinker System]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Q235 Galvanized Pipe]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Best Hot 120 Gas Pump Nozzle Fuel Injector Nozzle]

Bobby Flay Websites Restaurant Martha Stewart Designer Tray, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling]

Bobby Flay Websites Restaurant Martha Stewart Designer Tray,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Rectangular Stainless Steel Pipe SS304]

Bobby Flay Websites Restaurant Martha Stewart Designer Tray, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber, Part I.Diameter 2.68m Paragraphed Autoclave for AAC Bricks. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Stainless Steel Ozone Disinfection Machine Ozone Generator]

Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Travelling Weekender Mens Canvas Duffle Gym Sports Bag.4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box, 175-184[Portable Concrete Mxier]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ISO 3821 3/16′′ Twin Line Welding Hose for USA Market]

Whitaker,J.R.(1963),China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC). Analytical Chemistry35(12), 1950-1953[ Livestock]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Komatsu Shantui Shehwa Zoomlion Liugong Dozer Steering Valve (16Y-76-22000 144-40-00100)Author for correspondence