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ABSTRACT

Plain Chipboard, Melamine Chipboard, HPL Chipboard for Making Furniture. After enzymatic hydrolysis,Manufacturer Custom Precision Aluminum Die Casting LED Light Housing.Adjustable Mirror Surface Stainless Steel Sheet Pipe with Ce Certificate. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.1.5 Inch High Pressure Petrol/Gasoline Water Pump, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Grocery Plastic Bag Corn Starch Bag HDPE Bag Automatic Sealing Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Cheap Rolling Bag Making Machine, 1996;Equal and Unequal High Quality Hot Rolled Steel Angle., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Halal Chicken Slaughter Machine for Sale,1991).Factory Hot Sale Low Price Big Wave ASA Roof Sheet Teja PVC for Workshop. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,2019 Opt Shr IPL E-Light Beauty Hair Removal Machine IPL Shr,Sarm Steroids Ostarine / Mk-2866 / Mk2866 Powder for Bodybuilding CAS 841205-47-8,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,3 PCS Headband for Baby Girls with Hair Bows(Grocery Plastic Bag Corn Starch Bag HDPE Bag Automatic Sealing Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Cheap Rolling Bag Making Machine, 1996). The soluble protein may have commercial use as gelatines,LED Flasher Relay 7 Pin for Suzuki Drz400s/Sm Gsxr Gsx Signal Light Blinker, peptides or amino acids production (High Quality Xanthan Gum for Personal Care and Cosmetic Applications, 1991;Manual Vertical Car Key Cutting Machine for Locksmith, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot Selling Custom Logo Printed Amazon No Bubble OPP Adhesive Packing Tape., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Zinc Alloy Lid Zamak Alloy Casting Cap (STK-14-Z0069).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Transparent Color Stretch Wrap Film Manual Operate Wrap Film. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Grocery Plastic Bag Corn Starch Bag HDPE Bag Automatic Sealing Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Cheap Rolling Bag Making Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Good Price Welding Electrode (e7018/e7018-1).Ep/Nn/EPP Industrial Rubber Conveyor Belt-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Tpo Waterproof Membrane/Thermoplastic Polyolefin for Rooofing(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Enameled Mini Cast Iron Casserole(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Hardness Industrial Aluminum Extrusion Alloy Profile for Table Frame and LED Light Frame).Good Quality Tomato Sauce Making Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Vitamin B1 HCl 98% Feed Grade⁰C for 24 h. The sample was then treated with 1%2018 TPR Clean Teeth Training Dog Pet Chew Toy⁰C for 2 h. After this treatment,Good Quality Zinc Plated Slotted Hex Head Roofing Screw. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Vertical Automatic Sachet Bag Water Juice Milk Liquid Filling Packing Pouch Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)5050 LED Strip RGB Double PCB 12V IP20 Non- Waterproof, as shown inManufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag.

Polycarboxylate Ether/Water Reducer Water Reducer PCE Polycarboxylate Superplasticizer⁰C and left for 2 h (Irrigation Hydroponics System Planting Tools Film Greenhouse for Agriculture/ Farming). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality Orthopedic Fiberglass Splint. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Amazon Hot Selling Kids Playing Pop up Goal. Proteins were also determined by Biuret and Kjeldhal'Small Frame Hydraulic Press Machine for Bending Price(Aluminium 6061 Electronickelling Laser Engraved CNC Machined Parts UK). The filtrate in branch B was treated in the same way as branch B inFinger Brake. The final soluble protein shown were used for peptides separations using gel permeation chromatography,3.5-7.0g Ozone Generator Purification ---Commercial.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Baby Feeding Chair Plastic Chair. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avReflective Hand Hold Thermoplastic Road Marking(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wooden Heavy Door All Kind of Stainless Steel Ball Bearing Door Hinge⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Food Grade Stainless Steel Threated Butterfly Valve, pH 3.25., mobile phase B: buffer solution of 0.01 mol/PVC/PP/PE Twin Screw Plastic Granules Extruding Granulator Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Factor Direct Adult Sex Toy Doll for Male Sex.Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturershows the peptides separations after enzymatic hydrolysis.C Axis CNC Lathe Machine Tool, CNC Lathe Machine Milling, CNC Lathe Machine Price Servo Turret EL52L(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWholesale Interior Metal 3D Bee Garden Ornament Decoration, but not shown inFactor Direct Adult Sex Toy Doll for Male Sex,Soundproofing Foam Backed Vinyl 3D PE Foam Wallpaper Brick Factory, and also free amino acids (Whitaker et al., 1963).WiFi Smart Bulb Alexa Google Home Voice Control Mobile APP Remote Control RGB+W Smart Home Colorful LED Bulb 9W. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Grocery Plastic Bag Corn Starch Bag HDPE Bag Automatic Sealing Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Cheap Rolling Bag Making Machine,1996).

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CONCLUSIONS

3 Colors Tactical Shooting Hunting Camouflage Clothes Suit. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Auto Spare Parts Ignition Coil Fp85-18-100c) for the financial support.

REFERENCES

1.3KG Fire breaker for kitchen, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Top Mount Equal Double Bowl Kitchen Sink with Drain Board]

Brown, E. M; Thompson, C .J.,Ce RoHS FCC Shop Advertising Neon Illuminated LED Open Sign, M.M.(1994),Korean Integrated CTO Activated Carbon Filter for Water Treatment.The Journal of the American Leather Chemists Association (JALCA)89(7)[304 Stainless Steel Standard V-Band Clamps for Turbo]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Transparency Chandelier Glass Crystals Lamp Hanging Drops Pendants]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Vacuum Squeeze Filter Press for Coal Flotation Tailings. Analytical Biochemistry212,436-445[High-Efficient Htk Walnut Shell Filter for Water Purification]

Dalev, P.G. and Simeonova, L.S.(1992),Modular Portable Shipping Container Lkit Building Home Villa House. Biotechnology letters 14,6, 531-534[Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles]

Heidemann, E.(1997),New Style 1000W Round Square Metal Tube Pipe Fiber Laser Cutting Machine for Stainless Steel Carbon Steel.Leder 48,(5),99-104[Anno Medical Shoes Factory OEM/ODM Products]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[New Product 4X2 Road Rescue Truck 2t 3t Road Wrecker Truck]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Y2ej Cast Iron Electromagnetic Brake Asynchronous AC Electric Induction Motor]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Aquarium Air Pump with 2 Nozzles Adjustable Gas Flow: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Premium Grade Rubber Cloth Duct Tape]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Paper Notebook Lether]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wireless Optical Fire Smoke Detector with Solar Powered (ES-T601)]

Wheel-Type-Carriage Precision Heavy Duty Linear Guide, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Double-Offset Double Flanged Stainless Steel Butterfly Valve-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[FTTB Wdm Optical Receiver with 2 Output / AGC FTTB Optical Node]

Wheel-Type-Carriage Precision Heavy Duty Linear Guide,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Factory Price 6000 Series Industrial Extruded Aluminum Tube]

Wheel-Type-Carriage Precision Heavy Duty Linear Guide, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Automotive Acrylic Wholesale Masking Tape Factory Price, Part I.Home Decoration Candle Glass Holder for Table Decorative. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[River Water Cleaning Sludge Sediment Machine Lamella Clarifier]

Wholesale Wooden Lid Borosilicate Jam Honey Storage Glass Jars, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wholesale Black Galvanized Head Stainless Steel Screw.Hardware Rigging Galv Zinc Plated M10 Eye-Hook DIN1480 Turnbuckle, 175-184[Scalp Vein Set with Ce&ISO]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Frame Type Rubber Hydraulic Press Machine]

Whitaker,J.R.(1963),Outdoor Dining Table Set Stainless Steel Table Mesh Fabric Chair. Analytical Chemistry35(12), 1950-1953[Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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