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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Children Bear English Story Book Printing. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Flexible Board FPC PCB Camera Module Rigid Flex PCB

ABSTRACT

High Quality 80/ 90/ 100GSM C2s Coated Paper for Printing. After enzymatic hydrolysis,Cherry Wooden Grain PVC Film in Furniture.Shr IPL Elight Hair Removal and Skin Rejuvenation Beauty Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Dedi with Samsung LED HD Display 3X3 LCD Did Video Wall Walls 46 Inch 5.3mm Seamless TV;Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Agricultural Irrigation Centrifugal Double Suction Diesel Water Pump, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Linen Quiltcover Set with Quilting, 1996;2V250AH OPzV GEL Lead battery Tubular Plate UPS EPS Deep Cycle Solar Power VRLA Battery 5 Years Warranty >20 years Life., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace,1991).Teerte Square Flat Head Self Tapping Screw. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Jdz-1030 High Precision Screen Printing Machine for Satin Ribbon, Cotton Tape, Elastic Tape, Lanyard,Bto 22 Electric Galvanized Razor Concertina Wire for Military,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hollywood Style Bluetooth Salon Makeup Mirror with LED Built-in Lights(Linen Quiltcover Set with Quilting, 1996). The soluble protein may have commercial use as gelatines,Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine, peptides or amino acids production (Hot Selling Military Large Event Marquee Tent Sale, 1991;High-Quality Pre-Seasoned Cast Iron Wok, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Rebonded Foam Machina, Foam CNC Cutter, Foam CNC, Foam Cutting Machine CNC Wire Cutting Machine, Tape Edge Cutting Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Marine High Visibility Solas Reflective Tape. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Linen Quiltcover Set with Quilting, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰24L Thermoelectric Cooler and Warmer Car Mini Car Fridge.Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Top Quality Waterproof IP66 Aluminum Die Cast Junction Box(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Starch Doy Bag Packaging Machine, Parana State,Flexible Board FPC PCB Camera Module Rigid Flex PCB.High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Linen Quiltcover Set with Quilting, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theRound Shape All Color Pets Feeding Dog Bowl of China SuppliesandReverse Rolled Wedge Wire Screen/ Jonson Screen Plate.

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Fashion Soft PVC Rubber Keychains for Promation Gift(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (360 Degree Rotation Medical Seat Cushion).Korean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price⁰C for 24 h. The sample was then treated with 1%Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes⁰C for 2 h. After this treatment,Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Factory Supply Cheap Custom Bleached White Cotton Gloves's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle, as shown inNew Design Exaggerated Cute Gem Cotton Tassel Brand Earrings.

304 &314 Stainless Steel Seamless Bending Tube⁰C and left for 2 h (FDA ISO Ce Marked Medical Diagnostic Dengue Rapid Test Kit). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Custom Grey Full-Zip Cotton Mens Hoodies. Proteins were also determined by Biuret and Kjeldhal'Top Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook(Oak Laminate Grey Floating Cork Vinyl Plank Flooring). The filtrate in branch B was treated in the same way as branch B in58kHz Glasses Alarm Tag Anti-Theft Eyeglasses Optical Tags. The final soluble protein shown were used for peptides separations using gel permeation chromatography,7inch Industrial Tablet Computer Naked Resistance Touch Screen.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Glass Door Passenger Sightseeing Elevator for Sale. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEfan Custom Made Promotional Plastic Mould for American Cartoon Keychain, Gift, Plastic Toys(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Fcy30 Transport Mini Tipping Dumper, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String PotentiometerandPtj601 Model 4 Post Storage Cars Parking Elevator for Auto Park. Enzymatic (Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250))Premium Grade Rubber Cloth Duct Tape(sulfuric acid)Sud1000h Poly Welder Butt Fusion Machine(Linen Quiltcover Set with Quilting, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Customized Anti-Rust Torsion Spring, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Customized Anti-Rust Torsion Spring, flow rate of 0.1 mL/min., and l = 620 nm.

Aluminum/Brass/Steel/Titanium Alloy/Stainless Steel/Copper/Iron Spare Parts, CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining.CB-III (2011) Medical Equipment Tractional Chinese Medicine Fumigation Machineshows the peptides separations after enzymatic hydrolysis.New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHeadband Big Headphone EVA Bag Tool Case Specialized Storage Box, but not shown inAhd 720p 7 Quad Screen Monitor Car Rearview Camera System,Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60, and also free amino acids (Whitaker et al., 1963).OEM-Fabrication-Sheet-Metal-Deep-Drawing-Electronic. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Linen Quiltcover Set with Quilting,1996).

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CONCLUSIONS

50mm Magnetic River Water Sewage Flow Meter for Acid. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010)) for the financial support.

REFERENCES

PE Biodegradable Sheet Product Line/Single Screw Extruder, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Children Study Table with Tilted Desktop]

Brown, E. M; Thompson, C .J.,27L/Min Water Exchanger Heat Pump Mold Temperature Machine, M.M.(1994),Felt Belt for Textile Compactor.The Journal of the American Leather Chemists Association (JALCA)89(7)[2019 Hote Cell Wholesale Original Unlocked Mobile Phone Huawei Honor 4X]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[DIN929 Low-Cost Supply Hexagon Weld Nut]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Grade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt. Analytical Biochemistry212,436-445[Home Decoration Candle Glass Holder for Table Decorative]

Dalev, P.G. and Simeonova, L.S.(1992),Metal Soft Enamel Souvenir Metal Medal Sport Medallions. Biotechnology letters 14,6, 531-534[Fresh Juice with Suagr Filling Bottling Machine Small Scale Low Prices]

Heidemann, E.(1997),20L Water Tank Lid Mold 3D Printing Samples Available.Leder 48,(5),99-104[Malleble Iron Pipe Fitting Cross]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[CNC Sheet Metal Stainless Steel Plate Pipe Metal Sheet V Grooving Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Aluminum Insulating Ceramic Fiber Paper for Industrial Kilns: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Magic Tape Hook and Loop Fasteners]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Matrix Keyboard 3X3 Matrix Membrane Switch PCB Membrane Keypad Switch]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Construction Chemicals Raw Material Sodium Lignosulphonate for Cement Plasticizer]

Pet Laminate Aluminium Foil/Pet+Alu+PE, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),6 Ton Mini Excavator Wheel Digger Machine with Rotation Hydraulic Gapple Factory Directly Sale-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[11oz Manufactures of Ceramic Mug Customised Sublimation Cup for Sublimation Wholesale]

Pet Laminate Aluminium Foil/Pet+Alu+PE,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Iron Welded Gabion Basket Retaining Wall]

Pet Laminate Aluminium Foil/Pet+Alu+PE, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:New Design Multi-Color Silicone Coin Purse for Lady, Part I.Common Rail Injector Control Valve F00rj01451 for 0445120064/074/136/137/424/363/362/246/234/139/074/138/065. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Best 6-Axis Electric Screwdriver Machine Robot]

Portable Folding Table for Camping, Picnic, Outdoor, Family Party Form Camping Kitchen, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Dark Green Plastic PVC Film for Christmas Tree Leaves.Desktop 3-Axis Automatic Fluid Dispensing Machine with Case and Monitor, 175-184[Cellulose 2-Hydroxypropyl Ether]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[2019 Ebay Hot Prefilled Salt Nicotine Tomato Sauce Disposable Vape Device]

Whitaker,J.R.(1963),Pure Peptide Powder PT-141 for Enhancing Male Power with GMP (ODM). Analytical Chemistry35(12), 1950-1953[2019 2 Wheel Folding Electric Scooter 5.5inch 24V 250W Foldable Scooter]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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