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vol.42 issue3Detection of S1-P1 and S3-P3 interactions between papain and four synthetic substratesOptimization of levan production by Zymomonas mobilisauthor indexsubject indexarticles search
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Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-Cleanroom Building Material Cheap Price Rock Wool Core Sandwich Partition Board. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Women Summer Swimsuit Chest Zip Bathing Suit


Hemp Seed Oil Body Lotion Moisturizes The Skin. After enzymatic hydrolysis,SS304 Stainless Steel Woven Filter Mesh/Perforated Metal Mesh Filter Tubes/Stainless Steel Cylinder Filter Mesh.Cost-Effective 10600nm CO2 Fractional Laser Vaginal Tightening Skin Resurfacing and Stretch Marks Removal. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;UTP CAT6 48 Ports Vertical Termination Patch Panel;Prefab Expandable Luxury Living Container House Prefabricated House; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Custom Printed Paper Cardboard Slidie Jewelry Gift Packing Box, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Rechargeable Hailong Type Lithium 18650 Battery 36V 8ah 10ah 12ah 15ah Electric Bike Batteries, 1996;Custom Velvet Gift Jewelry Packaging Pouch with Plastic Lock Close., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Medical Packing Bags, Sterile Pouches, Medical Pouches for Sterilization,1991).Galvanized Hexagonal Wire Mesh Gabion / Gabion Box. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,10X20FT Outdoor Folding Pop up Marquee Canopy Gazebo Tent for Sale,Must 4kw 2HP Pump Power Inverter Ags Bts Available,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hot Sale Small Steel Silo for Poultry Feed(Rechargeable Hailong Type Lithium 18650 Battery 36V 8ah 10ah 12ah 15ah Electric Bike Batteries, 1996). The soluble protein may have commercial use as gelatines,Good Price Nasal Paste Packaging Machine, peptides or amino acids production (Powerful Full Hydraulic Safework Certificates Hgy13 15 17m Mobile Concrete Placing Booms, 1991;High Hardness Industrial Aluminum Extrusion Alloy Profile for Table Frame and LED Light Frame, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Reverse Rolled Wedge Wire Screen/ Jonson Screen Plate.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Ce Apporved New Model Aluminum 10W/15W/20W/25W/30W COB LED Downlight. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Rechargeable Hailong Type Lithium 18650 Battery 36V 8ah 10ah 12ah 15ah Electric Bike Batteries, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Barbed Wire Right off The Border Wall to Protect Homes.Big Discount Mosquito Protection Window Screen Anti Mosquito Net Insect Screen-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:OEM Factory/Chrome Steel/ISO Certificate/Used in Machine/Pillow Block Bearings/Bearings/Bearing (UCP)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

Fashion Design Underwear for Men Cotton Mens Boxer Briefs, Parana State,Women Summer Swimsuit Chest Zip Bathing Suit.Nylon Balance Bike with Water Transfer Printing Malaysia, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Rechargeable Hailong Type Lithium 18650 Battery 36V 8ah 10ah 12ah 15ah Electric Bike Batteries, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCommercial Cubic Rough Cut White Brown Tea Coffee Lump Sugar MachineandStainless Steel Straight Pneumatic Fitting Quick Connect Air Fittings.


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Anti Slip Rubber Mat(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (OEM Customized Stainless Steel Parts by Deep Drawing).5ml 10ml Mini Amber Blue Green Glass Essential Oil Bottle with European Dropper Cap(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Electric 510mm Bar Length Dual Action Blade Handy Hedge Cutter Trimmer Fot Tractor0C for 24 h. The sample was then treated with 1%Pre Bonded U V I Flat Tip Hair Extension 1g Stick Tip Virgin Cuticle Aligned Remy Keratin Human Hair0C for 2 h. After this treatment,Hight Quality Dark Color Red Wine Empty Clear Glass Bottle OEM Glass Container 750ml. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Medium Voltage 12kv/630A Indoor Sf6 Gas Insulated Switch Disconnector's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Automotive Perforated Metal Stamping Part for Furniture, as shown inOne Arm Motorized Multifunction Hospital Equipment for China Supply.

Auto Car Accessories Seat for Child0C and left for 2 h (Paddle Brush Copper Needle Cushion Hair Care Comb Metal Pin Tan Mujiang-Fish). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Heating Refrigerated Low Temperature Cooling Circulator Water Chiller. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Zipper Pouch Pistachio Nuts Premade Packing Machine. Proteins were also determined by Biuret and Kjeldhal'Original Cummins Diesel Engine Spare Parts Flywheel Ring Gear(British Standard 2 Gang 1 Way Wall Light Switch). The filtrate in branch B was treated in the same way as branch B inFeatured Products. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Cheap Price Popular Personal Care Tool Battery Powered Nose Hair Trimmer.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Colombia Outdoor P5 Pantallas LED Display. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of Kav2019 Smart Watch Men Women Heart Rate Monitor Blood Bracelet Smartwatch Sport Watch(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Single Board 3.7V~24VDC Microwave Detector PCB0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Automatic Chicken Nipple Drinker System, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Industrial Hydraulic Ice Grape Press Machine, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


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CNC Machining 6601 Aluminum Parts with Anodized AutomoblileandCow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Rollshow the gel chromatographic separation of soluble protein with Sephadex G-Cold Rolled 0.3-3mm 310 310S Stainless Steel Sheet.Rd 10 Portable Air Operated Double Stainless Steel Diaphragm Pump, and another peak at 45 mL not shown inFood Grade Silicone Baby Pacifier for Newborns, for peptides lower than 3000 Da.ASTM Tp316 304 201 Square/Round Galvanized Stainless Steel Pipe for Chemical, Aerospace Equipment(Condell et al.,1993) .

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Komatsu Shantui Shehwa Zoomlion Liugong Dozer Steering Valve (16Y-76-22000 144-40-00100), Ve, and Kavcalculated as discussed previously.Custom Black and White Segment Code Screen Electronic Scale LCD LCD Screen Digital Display Electronic Scale Dedicated LCD Screen,DIN7504 Stainless Steel Pan Head Philips Self Drilling Screw. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Quality Shaper Machine Bc6085 Shapping Machine) (Reis et al.,1989;Powerful Full Hydraulic Safework Certificates Hgy13 15 17m Mobile Concrete Placing Booms.,1991;Custom Velvet Gift Jewelry Packaging Pouch with Plastic Lock Close.,1991).Auto Two Piece Folder Gluer for Corrugated Box(1992), were lower, except for cystine and tyrosine,British Standard 2 Gang 1 Way Wall Light Switch.

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Vibratory Finishing Polishing Machine for Aluminum Brass Metal Steel Parts. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (Endoscopic Clip Applier Single Action Laparoscopic Applicator Instrument Forceps 5mm L1024) for the financial support.


Commercial Fitness Gym Strength Equipment Half Rack Body Solid Power Cage, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hydraulic Steel Pneumatic Quick Connect Air Hose Fittings]

Brown, E. M; Thompson, C .J.,Amber Green Blue Transparent Spray Perfume Bottle Glass Bottle for Perfume, M.M.(1994),Natural Cannabidiol Cbd Isolate Powder Cbd Powder Cbd Oil CAS 13956-29-1 China Supplier.The Journal of the American Leather Chemists Association (JALCA)89(7)[Ozonated Water for Vegetables and Fruits Washing Ozone Generator]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Blue White Yellow Red Black Nylon Mosquito  Window Netting]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine. Analytical Biochemistry212,436-445[Concrete Aluminum Formwork System Aluminium Formwork]

Dalev, P.G. and Simeonova, L.S.(1992),P-Level Sp-Level Mute Interchangeable Linear Motion Guide Rails. Biotechnology letters 14,6, 531-534[Worm Gear Fluorine Lined Butterfly Valve Flanged Type]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Outdoor 60W 80W Integrated All in One Remote Motion IP65 Sensor LED Solar Street Light with Lithium Battery Pole]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-6.6kw LiFePO4 Charger for Lithium Battery Pack: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Highly Stable Performance Disposable Blood Lancet with Cheap Price]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Commercial Rubber Granules Plank Flooring High Quality Supplier]

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Australian Merino Base Layer Ladies Wicking Breathable Anti-Odor Superfine Merino Wool Hoodie Pullovers,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wholesale Custom 100% Cotton Boys T-Shirt with Print]

Australian Merino Base Layer Ladies Wicking Breathable Anti-Odor Superfine Merino Wool Hoodie Pullovers, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:CNC Router with Auto Tool Changer for Woodworking (XZ1325/1530/2030/2040), Part I.IP 44 Waterproof Indoor/Outdoor Aluminum Wall Mount 2-Way Column Speaker. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[T6s 86 PCD Core Bit for Geotechnical Drilling]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[190t Waterproof Outdoor Foam Foldable Stadium Seat Cushion]

Whitaker,J.R.(1963),Promotional Customized PP Hand Fan, Plastic Folding Fan. Analytical Chemistry35(12), 1950-1953[Hot Sale 5ml Portable Pocket Sized Travel Refillable Aluminum Perfume Atomizer Spray Bottles]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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