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ABSTRACT

DIN Standard Gratings/Gavlanized Steel Grating. After enzymatic hydrolysis,50mm Magnetic River Water Sewage Flow Meter for Acid.PE Biodegradable Sheet Product Line/Single Screw Extruder. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Grade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Metal Soft Enamel Souvenir Metal Medal Sport Medallions, 1996;20L Water Tank Lid Mold 3D Printing Samples Available., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wholesale High Quality Metal Car Mount Holder Phone Holder,1991).Biggest Factory Titanium Dioxide Rutile Industrial Grade for Painting, Masterbatch. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Aluminum Insulating Ceramic Fiber Paper for Industrial Kilns,Pet Laminate Aluminium Foil/Pet+Alu+PE,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,6 Ton Mini Excavator Wheel Digger Machine with Rotation Hydraulic Gapple Factory Directly Sale(Metal Soft Enamel Souvenir Metal Medal Sport Medallions, 1996). The soluble protein may have commercial use as gelatines,New Design Multi-Color Silicone Coin Purse for Lady, peptides or amino acids production (Common Rail Injector Control Valve F00rj01451 for 0445120064/074/136/137/424/363/362/246/234/139/074/138/065, 1991;Portable Folding Table for Camping, Picnic, Outdoor, Family Party Form Camping Kitchen, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Dark Green Plastic PVC Film for Christmas Tree Leaves., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Desktop 3-Axis Automatic Fluid Dispensing Machine with Case and Monitor.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Pure Peptide Powder PT-141 for Enhancing Male Power with GMP (ODM). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Metal Soft Enamel Souvenir Metal Medal Sport Medallions, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰1X4mm2 (12AWG) Solar Cable Red or Black PV Cable Wire Copper Conductor XLPE Jacket TUV Certifiction.Water Treatment Anthracite-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Heat Treated Welding Protection Glassfiber Cloth Ht800(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality Sheet Metal Bending Roll Forming Machine).Injector Pump Calibration Test Bench(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%ASME/ASTM Butt-Welding Stainless Steel Plate Flange⁰C for 24 h. The sample was then treated with 1%500L Unitank Fermenter (EV 500L, TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage Tank⁰C for 2 h. After this treatment,Stainless Steel Straight Pneumatic Fitting Quick Connect Air Fittings. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Polyester / Polypropylene Woven Geotextile for Road Construction's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Granite Stone Jaw Crusher of Mining Machine, as shown inKids Thermal Insulated Bento Bag Children Picnic Lunch Cooler Bag (BC0089).

Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor⁰C and left for 2 h (Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Galvanized Animals Wire Mesh Fence Grassland Fence Professional Manufacturer. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine. Proteins were also determined by Biuret and Kjeldhal'Orange Color Long Style Cotton Drawstring Bag, Gym Sport Backpack, Gift Bag(Full Color Child Book Printing Service). The filtrate in branch B was treated in the same way as branch B in4.0 Inch IPS Screen 1080P Dash Cam with 3 Channel Cameras Inside and Front Camera DVR Black Box. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Wholesale All Kinds Cheap Plush Animal Baby Slippers.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Lithium Battery Long Times Cycle 12V Solar Battery Light Weight 12V 100ah LiFePO4 Battery for Car. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMovable Folding Knuckle Boom Hydraulic Truck-Mounted Crane(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.New Arrive Customized Color Box Compress 10 Inch Visco Gel Memory Foam Mattress⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Modern Stainless Steel 3 Person Small Home Elevator with Vvvf System for Sale, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Taiding Custom Tank Sheet Metal Fabrication, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Rectangular Box Facial Tissue Paper Fk-110. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Heat Pump Non-Programmable HVAC Room Thermostat) for the financial support.

REFERENCES

SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[8-800 Bar Output Pressure Gas Automatic Booster Pump]

Brown, E. M; Thompson, C .J.,Digital Lock Electronic Door Lock Password Keypad Number for Cabinet Door, M.M.(1994),AAC Block Production Line / AAC Equipment Manufacturing/ Machinery.The Journal of the American Leather Chemists Association (JALCA)89(7)[Full Automatic Carbonated Water Filling Full Line]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High-Quality Pre-Seasoned Cast Iron Wok]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-CB-III (2011) Medical Equipment Tractional Chinese Medicine Fumigation Machine. Analytical Biochemistry212,436-445[New Arrive Customized Color Box Compress 10 Inch Visco Gel Memory Foam Mattress]

Dalev, P.G. and Simeonova, L.S.(1992),Q32 Series Tumble Belt/Crawler Shot Blasting Machine. Biotechnology letters 14,6, 531-534[Buy Single Item Moving Bee Eye Light Lowly Price]

Heidemann, E.(1997),Galvanized Bar Grating.Leder 48,(5),99-104[Louver Shutter Automatic]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Silicone Reusable Food Storage Bag China Manufacturer]

Industrial Carbon Steel Wire Hose Hydraulic Pipe, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Quality Custom Self Adhesive Leather Patch for Jackets.Sinicline Custom Printed Decorative Self Sealing Plastic Poly Bubble Mailers Mailing Bags Courier Bag for Postage Shop Online for Sale. USP/SP[Cast Iron Butterfly Valve with Handles]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Products Genuine Leather En345-1 Steel Toe Cap Leather Safety Boots]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-380mm Working Area Concrete Diamond Floor Edge Grinder: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[U. S. Type Drop Forged Wire Rope Clip]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[50 Foot Hdmi Cable Wholesaler Quotes & PriceList]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Fashion Men′s Clothing Shop Interior Design Clothes Store Fixtures]

Angle Iron Steel Purlin Q235/345 Equal Angle Steel / Hot Dipped Galvanized Beam, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),New Mobile Smartphone for Xiaomi 9 PRO, 5g Android Phone, 8GB 256GB Unlocked Mobile Phone Wholesale for Huawei Series Mate-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[SGS Steel Lab Acid and Alkali Resistance Fume Hood Laboratory Furniture with Ce Certification (JH-FC019)]

Angle Iron Steel Purlin Q235/345 Equal Angle Steel / Hot Dipped Galvanized Beam,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[4000W Industrial High Power Pipe Plate Whole Cover Exchange Platform Metal Fiber Laser Cutter for Carbon/Aluminum/Stainless/Brass 3015gr]

Angle Iron Steel Purlin Q235/345 Equal Angle Steel / Hot Dipped Galvanized Beam, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Discussion and Interpretation System, Part I.7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wholesale All Kinds Cheap Plush Animal Baby Slippers]

Frozen Soybean in Pod, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Custom Sheet Metal Stamping Bending Product Manufacturing Service.Waterproof EVA Rubber Roofing Material for Shingle Building, 175-184[304 316 Sanitary Stainless Steel Colloid Mill Manufacturer in China]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[12ml Round Shape Empty Clear Nail Polish Glass Bottle]

Whitaker,J.R.(1963),OEM Gray Cast Iron Sand Casting Products with Universal Parts. Analytical Chemistry35(12), 1950-1953[Diamond Segments 2000mm Diameter Granite Cutting Blade]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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