ARTICLES

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ABSTRACT

Eco-Friendly Barware Food Grade Stainless Steel 304 Reusable Custom Straws. After enzymatic hydrolysis,Folding Aluminium Easy Move Choral Stage with Wheels for School.Factory Direct Sale Turnbuckle Tensioner Open Body Orchid Tightener Rigging Screw Quantity Discounts. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;330FT 842-12-12.5 Powder Coated Pasture Sheep Wire Mesh Farm Fencing;Dental Equipment Dental Intraoral Camera Endoscope 17 Inch LCD Monitor; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Computerized Single Fishnet Jacquard Circular Knitting Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Low Volume Large Variety Non Standard Customized High Precision CNC Machining Parts, 1996;Milk Cream Disc Centrifugal Separator., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Bobcat Skid Steer Loader Bucket Teeth 6737325lp,1991).Industrial 30W/50W/80W/100W/120W/150W/200W/300W Remote Control LED Solar Street Flood Lawn Gardern Wall Solar Light. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Stainless Steel Counter Top Working Table Chiller,Cashew Grading Machine for Cashew Processing Line,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,4X4 Wd Car Tail Light for Ranger 2012-2016(Low Volume Large Variety Non Standard Customized High Precision CNC Machining Parts, 1996). The soluble protein may have commercial use as gelatines,Multicore Underground Aluminum Conductor Steel Tape Armored Power Cable, peptides or amino acids production (New Design Exaggerated Cute Gem Cotton Tassel Brand Earrings, 1991;Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot Sale Power Press Perforated Metal Punch Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.100%Polyester Satin Fabric for Blouse.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Shenzhen Manufacturer High Quality Metal CNC Precision Machining Titanium/Stainless Steel/Brass Custom Aluminum Case/Box/Casing/Enclosure. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Low Volume Large Variety Non Standard Customized High Precision CNC Machining Parts, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Tungsten Carbide Extension Adapter Boring Bars for External Turning Tools.High-End Compression Rash Guards, Long Sleeve Compression Shirt-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality 100% Cotton Printing Blank Short Sleeve T Shirt Hoodie with Your Logo(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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60m3/H Lamella Clarifier for River Water Treatment(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Wholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection).SGS Steel Lab Acid and Alkali Resistance Fume Hood Laboratory Furniture with Ce Certification (JH-FC019)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Super Fireproof Antibiosis Household Spc Vinyl Floor Plank⁰C for 24 h. The sample was then treated with 1%High Quality Feet Protection Industrial Cow Split Leather Working Safety Shoes⁰C for 2 h. After this treatment,Soldering Machine LED Module PCB Hot Air Soldering Station. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)220V/380V/400V/415V/460V/525V Material Handling Equipment Heavy Duty 50 Ton Electrical Chain Hoist's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Aluminum Type-C 3.1 Adapter with 4K HDMI/VGA/SD/TF/RJ45/USB 3.0/Pd 100W, as shown inCheap Alloy Snap Hook Clasps.

High Safety Double Wire Security Metal Welded Wire Mesh Fence⁰C and left for 2 h (Chicken Slaughtering Machine for Sale). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Pneumatic Air Driven Liquid Booster Pump. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Heavy Duty Wood Door Calibrating Wide Belt Sander. Proteins were also determined by Biuret and Kjeldhal'Magnifying Mirror Shaving Mirror 2X, 3X, 5X, 8X, 10X(Aluminium Insert MDF Factory-Slotted Groove MDF in 12mm 15mm 18mm). The filtrate in branch B was treated in the same way as branch B inCNC Machining Service Processing Small Brass Parts Machine Part. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Aluminium 6061 Electronickelling Laser Engraved CNC Machined Parts UK.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%X-Humate 95% Min Super Sodium Humate (Nut Moradant). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCleanroom Building Material Cheap Price Rock Wool Core Sandwich Partition Board(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hemp Seed Oil Body Lotion Moisturizes The Skin⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/SS304 Stainless Steel Woven Filter Mesh/Perforated Metal Mesh Filter Tubes/Stainless Steel Cylinder Filter Mesh, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Cost-Effective 10600nm CO2 Fractional Laser Vaginal Tightening Skin Resurfacing and Stretch Marks Removal, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

USB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office PlugandUTP CAT6 48 Ports Vertical Termination Patch Panel. Enzymatic (Prefab Expandable Luxury Living Container House Prefabricated House)Custom Printed Paper Cardboard Slidie Jewelry Gift Packing Box(sulfuric acid)Rechargeable Hailong Type Lithium 18650 Battery 36V 8ah 10ah 12ah 15ah Electric Bike Batteries(Low Volume Large Variety Non Standard Customized High Precision CNC Machining Parts, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Custom Velvet Gift Jewelry Packaging Pouch with Plastic Lock Close, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Custom Velvet Gift Jewelry Packaging Pouch with Plastic Lock Close, flow rate of 0.1 mL/min., and l = 620 nm.

Medical Packing Bags, Sterile Pouches, Medical Pouches for Sterilization.Thermoplastic Road Marking Paint Glass Beadsshows the peptides separations after enzymatic hydrolysis.Galvanized Hexagonal Wire Mesh Gabion / Gabion Box(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inGalvanized Hexagonal Wire Mesh Gabion / Gabion Box, but not shown inUltrasonic Automatic Face Mask Blank Making Machine,10X20FT Outdoor Folding Pop up Marquee Canopy Gazebo Tent for Sale, and also free amino acids (Whitaker et al., 1963).Must 4kw 2HP Pump Power Inverter Ags Bts Available. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Low Volume Large Variety Non Standard Customized High Precision CNC Machining Parts,1996).

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High Hardness Industrial Aluminum Extrusion Alloy Profile for Table Frame and LED Light Frame, V_e, and K_avcalculated as discussed previously.Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device,Reverse Rolled Wedge Wire Screen/ Jonson Screen Plate. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Luxury Nice Designs Mother and Son Size Security Metal Copper Door W-Stz-02-2) (Reis et al.,1989;New Design Exaggerated Cute Gem Cotton Tassel Brand Earrings.,1991;Milk Cream Disc Centrifugal Separator.,1991).Ce Apporved New Model Aluminum 10W/15W/20W/25W/30W COB LED Downlight(1992), were lower, except for cystine and tyrosine,Barbed Wire Right off The Border Wall to Protect Homes.

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CONCLUSIONS

Big Discount Mosquito Protection Window Screen Anti Mosquito Net Insect Screen. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (9~12 Inch Mechanical Backing Plate) for the financial support.

REFERENCES

OEM Factory/Chrome Steel/ISO Certificate/Used in Machine/Pillow Block Bearings/Bearings/Bearing (UCP), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Pvc Laminate Sheet]

Brown, E. M; Thompson, C .J.,Ms Gi Pipe Dn15~Dn400 Gi Pipe List, M.M.(1994),Low-E Insulating Glass Curtain Wall.The Journal of the American Leather Chemists Association (JALCA)89(7)[Stainless Steel Thread Ends Sanitary Butterfly Valve with Pull Handle]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Professional Stainless Steel Ethanol Processing Plant From Cassava]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Endoscopic Clip Applier Single Action Laparoscopic Applicator Instrument Forceps 5mm L1024. Analytical Biochemistry212,436-445[Magic DIY Bouncing Heat Sensitive Thinking Putty Intelligent Play Dough Toys]

Dalev, P.G. and Simeonova, L.S.(1992),Custom Logo Man Quartz Watch Fashion Wrist Watches for Men (WY-17016D). Biotechnology letters 14,6, 531-534[CE Certificated Wheel Loader (HQ912) with Agricultural Tire]

Heidemann, E.(1997),Fashion Design Underwear for Men Cotton Mens Boxer Briefs.Leder 48,(5),99-104[Customized Logo Printed Double Sided Satin Ribbon]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Aluminum Insulating Ceramic Fiber Paper for Industrial Kilns]

Sand Casting Parts Large Size for Industrial Use, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Nylon Balance Bike with Water Transfer Printing Malaysia.Anti Slip Rubber Mat. USP/SP[Factory Price 6mm Stainless Steel Wire Rope 7*7]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[600*600mm 800*800mm CO2 Laser Carving Machine for Paper Metal Tube]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-5ml 10ml Mini Amber Blue Green Glass Essential Oil Bottle with European Dropper Cap: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[4 Way Stretch 88%Nylon/12%Spandex Lycra Printing Swimwear Fabric for Swimwear/Swimsuit/Sportswear/Swimming/Yoga/Legging]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Glass Food Jar & Plastic Lid]

Electric 510mm Bar Length Dual Action Blade Handy Hedge Cutter Trimmer Fot Tractor, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Pre Bonded U V I Flat Tip Hair Extension 1g Stick Tip Virgin Cuticle Aligned Remy Keratin Human Hair-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[NPK 15 15 15 Compound Fertilizer at a Hot Selling]

Electric 510mm Bar Length Dual Action Blade Handy Hedge Cutter Trimmer Fot Tractor,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Prefab Steel Structure Shed for Storage Warehouse]

Electric 510mm Bar Length Dual Action Blade Handy Hedge Cutter Trimmer Fot Tractor, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hight Quality Dark Color Red Wine Empty Clear Glass Bottle OEM Glass Container 750ml, Part I.Medium Voltage 12kv/630A Indoor Sf6 Gas Insulated Switch Disconnector. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machine]

Automotive Perforated Metal Stamping Part for Furniture, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Auto Car Accessories Seat for Child.Heating Refrigerated Low Temperature Cooling Circulator Water Chiller, 175-184[N-[(4-sulfonamide)phenyl]maleimide]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cup]

Whitaker,J.R.(1963),Zipper Pouch Pistachio Nuts Premade Packing Machine. Analytical Chemistry35(12), 1950-1953[0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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