ARTICLES

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ABSTRACT

100% Acrylic Knitted Sports Woven Scarf. After enzymatic hydrolysis,Prefab Steel Structure Shed for Storage Warehouse.Glass Manufacturer/Float Glass/ Tempered Glass (Flat or Curved) for Building / Furniture. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.3pH in 3 pH out High Frequency Online UPS - Modular UPS 10 -300kVA, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (1650mm*80mm Stable Power Laser Tube, 1996;FDA ISO Ce Marked Medical Diagnostic Dengue Rapid Test Kit., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Lead Ingot 99.994 Reasonable Price,1991).Micro-Combustion Tube, Test Tube (HK-224). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Stn Type 132X64 Round Display Small 2.1 Inch Cog DOT Matrix St7565r LCD Display Module Screen,OEM Service Machining Milling Turning Service Custom CNC Aluminum Case,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,3W LED Explosion Proof Emergency Exit Light(1650mm*80mm Stable Power Laser Tube, 1996). The soluble protein may have commercial use as gelatines,Multi-Layer Rigid-Flex PCB Fr4-Polyimide Material Sample Making, peptides or amino acids production (Stationery PP Clipboard With 7 Inch Scale Ruler, 1991;Automatic Food Cookies Cake Cartoning Machine for Big Size Carton Box, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Custom Blue Round Lid Spray Perfume Glassware Cosmetic Glass Bottle., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.PVC Cross Joint Pipe Fittings Equal Pipe Cross.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-3PC Stainless Steel Flanged End Ball Valve 3 Inches 150. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (1650mm*80mm Stable Power Laser Tube, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Ysdcnc Manual Sheet Metal Plate Bending Roller Machine.Betaine Anhydrous 98% Food Grade Good Quality Competitive Price-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:2019 Brand New Promotion Gift Custom Logo Printed Laptop Webcam Cover(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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750W 8X16 Precision Mini Metal Lathe 3 Jaw 50-2500rpm(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (65 Inch Smart Digital 3D 4K UHD Flat Screen Television Color LCD LED TV).Kindergarten Playground Preschool Kids Plastic Slide and Swing(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks⁰C for 24 h. The sample was then treated with 1%Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200⁰C for 2 h. After this treatment,Railway Freight Wagon Spare Parts Casting and Forging for Bogie. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Rainsky Ceiling Mounted Stainless Steel Shower Heads's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Custom Sizes Acrylic Shoes Storage Display Box, as shown inAngle Iron Steel Purlin Q235/345 Equal Angle Steel / Hot Dipped Galvanized Beam.

High Quality Casting Cover Aluminum Die Casting Products⁰C and left for 2 h (Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Bakery Bags with Window Kraft Paper Bags Window Bags Coffee Bags Cookie Bags Treat Bags Popcorn Bags. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser. Proteins were also determined by Biuret and Kjeldhal'SGS 0.2um 3.0 Micron PP Pleated 10 Inch Filter Cartridge for Water Treatment(SDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt Welder). The filtrate in branch B was treated in the same way as branch B inOem Dust Cover. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Furniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av0.005mm Tolerance CNC Turned Stainless Steel 304 316L Parts, CNC Lathe Machine Parts(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.304 316 Stainless Steel Grooved Cross for Water⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Outdoor 60W 80W Integrated All in One Remote Motion IP65 Sensor LED Solar Street Light with Lithium Battery Pole, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wear Resistant Rubber Ceramic Wear Linier Plate for Chute Liner) for the financial support.

REFERENCES

Automotive Bearings, Wheel Hub Bearing, Wheel Bearing (387144), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Bleached Bamboo Diffuser Reed Stick]

Brown, E. M; Thompson, C .J.,Garlic Red Mesh Bag, M.M.(1994),Z60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart Reminder.The Journal of the American Leather Chemists Association (JALCA)89(7)[Iron Sheet Metal CNC Press Brake]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Milk Cream Disc Centrifugal Separator]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Ground Finish Molybdenum Tzm Rod. Analytical Biochemistry212,436-445[(Trade Assurance) Hot Sale OEM Custom Made Women Yoga Spandex Shorts]

Dalev, P.G. and Simeonova, L.S.(1992),Transparent/Clear BOPP Jumbo Roll. Biotechnology letters 14,6, 531-534[Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments]

Heidemann, E.(1997),Yashi 19 270degree Pyramid Hologram Display Showcase Attractive.Leder 48,(5),99-104[Auto Parts Welding Jigs And Fixtures]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Small Blinking Battery Operated Luminary Plastic LED Tea Lights]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Transparency Chandelier Glass Crystals Lamp Hanging Drops Pendants]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-PVC Shrink Sleeve Label for Drinking Water Bottle: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High-Efficient Htk Walnut Shell Filter for Water Purification]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Aluminum Die Casting Process]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles]

Hang on Car Headrest Holder Tablet Cover Case for Xiaomi Pad 4, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Galvanized Square Wire Mesh, Metal Window Screen-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[New Product 4X2 Road Rescue Truck 2t 3t Road Wrecker Truck]

Hang on Car Headrest Holder Tablet Cover Case for Xiaomi Pad 4,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Good Quality PVC Plastic Ceiling for Wall Panel]

Hang on Car Headrest Holder Tablet Cover Case for Xiaomi Pad 4, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Qiankai Anti-Theft Burglarproof Unbreakable Stainless Steel Security Window Screen Factory, Part I.2019 2 Wheel Folding Electric Scooter 5.5inch 24V 250W Foldable Scooter. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Y2ej Cast Iron Electromagnetic Brake Asynchronous AC Electric Induction Motor]

OEM Customized Stainless Steel Parts by Deep Drawing, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Children Bear English Story Book Printing.Flexible Board FPC PCB Camera Module Rigid Flex PCB, 175-184[Best Cnc Milling Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Premium Grade Rubber Cloth Duct Tape]

Whitaker,J.R.(1963),High Quality 80/ 90/ 100GSM C2s Coated Paper for Printing. Analytical Chemistry35(12), 1950-1953[Wireless Optical Fire Smoke Detector with Solar Powered (ES-T601)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

FTTB Wdm Optical Receiver with 2 Output / AGC FTTB Optical NodeAuthor for correspondence