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ABSTRACT

High Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157. After enzymatic hydrolysis,12ml Round Shape Empty Clear Nail Polish Glass Bottle.M4X10 Round Head Core Pulling Aluminum Nail. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Standard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best Price, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (PVC Ceiling Wall Pannelling, Interior Decorative PVC Wall Panels, 1996;2017 New Style Modern Kitchen Cabinet Design Wooden Kitchen Furniture., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Imirootree 250ml Glass Jar with Lids for Cosmetic Cream,1991).Double Wine Carrier Bag Gift Box Tote. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,(Trade Assurance) Hot Sale OEM Custom Made Women Yoga Spandex Shorts,Huaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch Cord,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Flexible PVC Granules for Cable, Wire and Insulation(PVC Ceiling Wall Pannelling, Interior Decorative PVC Wall Panels, 1996). The soluble protein may have commercial use as gelatines,7 Ahd 1080P Rearview Camera with Quad Monitor Screen for Truck, Trailer RV Vision Security, peptides or amino acids production (Nc Duplex Cut-off & Nc Double-Layer Stacker, Used Cardboard Corrugator Line, 1991;Brass Pump Spare Parts for Circulating Pump, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Screw-in Brass Inserts for Plastic M4 M5 M6 M8 M10 Multiple Pack Sizes., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wall Mounting Full HD 1080P High Resolution 1280*800pixels Digital Photo Frame with HD-Mi.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Bosch Fuel Injector for Weichai Cummins Komatsu Engine 0445120123 Vg1038080007 Vg1560080276 095000-8011. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (PVC Ceiling Wall Pannelling, Interior Decorative PVC Wall Panels, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Lantern for Garden Decoration.Bending Sheet Metal Custom-Made Aluminum Bracket-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:New Shacman F3000 6X4 Truck Trailer Head(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

HOWO Sinotruck Heavy Truck Parts Air Spring Suspension Wg1642110024, Parana State,Gyc 2000L 100L Alcohol Liquor and Spirits Distilling Plant.Aluminum Truss System, Aluminum Lighting Truss, Roof Truss System for Event, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (PVC Ceiling Wall Pannelling, Interior Decorative PVC Wall Panels, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theIntegrated LED Solar Street Sensor Light with Sensor Control for Highway 10W/20W/30W/40W/50W/60WandWholesale Wireless Cheapest Android 2D Barcode Scanner.

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Mobile/Cell Phone LCD for iPhone 6s Plus Phone LCD Touch Screen(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High-Quality Pre-Seasoned Cast Iron Wok).Activated Carbon Strong Effect Peeling off Face Mask Mud(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Type Price Prefabricated Roof Tile in Sudan⁰C for 24 h. The sample was then treated with 1%Stackable Mahogany Color Wooden Chiavari Chair with Soft Cushion⁰C for 2 h. After this treatment,Factory Supply 100% Natural Epimedium Extract for Male Health. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Stainless Steel Bathroom Accessories 304 Polished or Brushed Finish No Chrome No Rust's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Type 0242 IP67 220V-415V 6h 3p+E 3pin 32A Industrial Plug, as shown inNew Arrive Customized Color Box Compress 10 Inch Visco Gel Memory Foam Mattress.

Top10 1080P H. 264 P2p CCTV WiFi IP Camera⁰C and left for 2 h (Buy Single Item Moving Bee Eye Light Lowly Price). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Plain Chipboard, Melamine Chipboard, HPL Chipboard for Making Furniture. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Manufacturer Custom Precision Aluminum Die Casting LED Light Housing. Proteins were also determined by Biuret and Kjeldhal'Adjustable Mirror Surface Stainless Steel Sheet Pipe with Ce Certificate(High Quality Silicone Reusable Food Storage Bag China Manufacturer). The filtrate in branch B was treated in the same way as branch B inCheap Price Large Wooden Jewelry Display Showcase for Shop. The final soluble protein shown were used for peptides separations using gel permeation chromatography,1.1mm to 8mm Waterproof Silver Mirror Glass for Home Decoration (M-S).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Full Color Child Book Printing Service. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av1.5 Inch High Pressure Petrol/Gasoline Water Pump(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Equal and Unequal High Quality Hot Rolled Steel Angle⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Halal Chicken Slaughter Machine for Sale, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Factory Hot Sale Low Price Big Wave ASA Roof Sheet Teja PVC for Workshop, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Manual Vertical Car Key Cutting Machine for Locksmith.Products Genuine Leather En345-1 Steel Toe Cap Leather Safety Bootsshows the peptides separations after enzymatic hydrolysis.Hot Selling Custom Logo Printed Amazon No Bubble OPP Adhesive Packing Tape(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inU. S. Type Drop Forged Wire Rope Clip, but not shown inFashion Men′s Clothing Shop Interior Design Clothes Store Fixtures,Zinc Alloy Lid Zamak Alloy Casting Cap (STK-14-Z0069), and also free amino acids (Whitaker et al., 1963).Transparent Color Stretch Wrap Film Manual Operate Wrap Film. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (PVC Ceiling Wall Pannelling, Interior Decorative PVC Wall Panels,1996).

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CONCLUSIONS

Promotional Custom Embossed Cups with Logo Print for Office/Holiday/Birthday. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Cylindrical Shape Beer Glass Bottle for Wine Storage) for the financial support.

REFERENCES

Enameled Mini Cast Iron Casserole, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[10w Floodlight]

Brown, E. M; Thompson, C .J.,Good Quality Tomato Sauce Making Machine, M.M.(1994),Vitamin B1 HCl 98% Feed Grade.The Journal of the American Leather Chemists Association (JALCA)89(7)[25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Large Batches Equipoise Light Yellow Liquids]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-2018 TPR Clean Teeth Training Dog Pet Chew Toy. Analytical Biochemistry212,436-445[Large Size Outdoor Store Front Signs Advertising Billboard]

Dalev, P.G. and Simeonova, L.S.(1992),Good Quality Zinc Plated Slotted Hex Head Roofing Screw. Biotechnology letters 14,6, 531-534[CNC Machining Metal/Aluminum/Brass Auto Parts]

Heidemann, E.(1997),Vertical Automatic Sachet Bag Water Juice Milk Liquid Filling Packing Pouch Machine.Leder 48,(5),99-104[40mm Thick Plywood High Quality Supplier]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Professional Manufacture Commercial Use Spice Grinder]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Motorcycle Accessories with MIM Part by Metal Injection Molding Technology with Precision and Complex Metal Parts]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Orthopedic Fiberglass Splint: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Korean Integrated CTO Activated Carbon Filter for Water Treatment]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Quality 5mm Sphere Neodymium Magnet for Kids′ Toy]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Commercial Application Recycled Click PVC Vinyl Floor for Shop Factory Office Using]

Amazon Hot Selling Kids Playing Pop up Goal, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Small Frame Hydraulic Press Machine for Bending Price-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[0.5 Gallons Cool Mist Ultrasonic Humidifier]

Amazon Hot Selling Kids Playing Pop up Goal,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Price]

Amazon Hot Selling Kids Playing Pop up Goal, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:3.5-7.0g Ozone Generator Purification ---Commercial, Part I.Baby Feeding Chair Plastic Chair. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Promotional Customized PP Hand Fan]

Reflective Hand Hold Thermoplastic Road Marking, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wide Jaws Adjustable Wrench with Big Grip Multi-Used (FM-W04).Wooden Heavy Door All Kind of Stainless Steel Ball Bearing Door Hinge, 175-184[Cabina de pintura de madera para hornear]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Auto Plastic Part Hardware Plastic Injection Mold]

Whitaker,J.R.(1963),Food Grade Stainless Steel Threated Butterfly Valve. Analytical Chemistry35(12), 1950-1953[Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Chemical Plant Transfer Industrial Water PumpAuthor for correspondence