ARTICLES

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ABSTRACT

100% Polyester Gabardine Shirting Fabric with Transfer PrintingTree. After enzymatic hydrolysis,School Kid Sport Travel Backpack Fashion Casual Promotional Bag (GB#20056).Fuguang High Quality Fb1015-280 280ml Double Wall Glass Water Bottle. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Lamella Clarifier for Industrial Wastewater Flocculation and Sedimentation;0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.125kHz RFID Hotel Key Card with T5577 Chip, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (USB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office Plug, 1996;Fashion Large Capacity Transparent PVC Leather Handbag Tote Shopping Bag., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Crop Fertilizer Agriculture Nh4cl Ammonium Chloride,1991).Wholesale High Pressure 80L Nitrogen Gas Cylinder. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,10mm Red Green Yellow Warning China Supplier Signal Lamp,Ms-184125-2 1.3mh Arnold Core Common Mode Choke Coils Toroidal Inductor,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hot Sale Thermostat Electric Heating Element for Solar Water Heaters(USB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office Plug, 1996). The soluble protein may have commercial use as gelatines,Phillip Countersunk Head Screw Hot DIP Galvanized, peptides or amino acids production (Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse, 1991;Cow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Heat Shrink Automatic Flow Wrapping Packing Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Ductile Iron Standard Brackets Pipe Hanger. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (USB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office Plug, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Luxury Vintage Throw Pillows Cover for Couch, Damask Pillows Cover, Decorative Pillow Covers.ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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AVR Se350 Automatic Voltage Regulator for Marathon Alternator(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Cummins L10 Engine Piston Cooling Nozzle 3070218).Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Excavator Heavy Duty Bolt on Right Hand Sidecutter 232-2147⁰C for 24 h. The sample was then treated with 1%High Strength Rigid PVC Plastic Foam Board for FRP⁰C for 2 h. After this treatment,Round, Hexagonal Hole Stainless Steel Perforated Metal Mesh for Building. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Threaded Stainless Steel Motorized Electric Floating 3PC Ball Valve, as shown inHigh Quality Xanthan Gum for Personal Care and Cosmetic Applications.

Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould⁰C and left for 2 h (Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.LED T20 Ba15D Indicator Lamps for Electrical Equipments, LED Ba15s/Ba15D Auto Bulbs, LED Bayomet Vehicle Lamps. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,New Style Polyester Duffle Bag for Travelling Trolley Bag Luggage Bag with 2 Wheels. Proteins were also determined by Biuret and Kjeldhal'Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck( Black Epoxy). The filtrate in branch B was treated in the same way as branch B inwww.speedlinerod.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%ADSS Newest Ultra Therapy Skin Tightening 3D Hifu. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Ceramic Chrome Hybrid Bearing Oil⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Medical Product Nonwoven Disposable Surgical Gown PP Isolation Gown, pH 3.25., mobile phase B: buffer solution of 0.01 mol/CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Wisdom LED Floor Lamp, Head Lamp Fabia Mining Lamp.Produce N2o for Medical Gas Equipmentsshows the peptides separations after enzymatic hydrolysis.18X16mesh Fiberglass Window Screen Insect Screen(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBobcat Skid Steer Loader Bucket Teeth 6737325lp, but not shown in2 Slots 40e2 Nice Industrial Machine LED Strip Aluminum Profile,Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch, and also free amino acids (Whitaker et al., 1963).Sterile Dsiposable Syringe for Dental 12ml. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (USB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office Plug,1996).

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Side Pack Manual Gas Engine Power Brush Cutter, V_e, and K_avcalculated as discussed previously.Punching Bending Welding Steel Work Fabrication,Black and White Cat Mobile/Cell Phone Cover/Case for iPhone 6/7/6plus/7plus. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Z60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart Reminder) (Reis et al.,1989;Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse.,1991;Fashion Large Capacity Transparent PVC Leather Handbag Tote Shopping Bag.,1991).Automatically Rotate The Makeup Brush with 360 Degrees and Multiple Gears(1992), were lower, except for cystine and tyrosine,1 Ton Chain Pulley Block with Geared Trolley.

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CONCLUSIONS

Powder Coating Polyester Resin with Factory Price. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Professional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine) for the financial support.

REFERENCES

Auto Plastic Part Hardware Plastic Injection Mold, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[PPGI Color Coated Steel Coil in Steel Suppliers]

Brown, E. M; Thompson, C .J.,Cheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machine, M.M.(1994),IEC 62196-2 Type 2 EV Charging 32A Male Plug.The Journal of the American Leather Chemists Association (JALCA)89(7)[Medium Voltage 12kv/630A Indoor Sf6 Gas Insulated Switch Disconnector]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality 100% Cotton Printing Blank Short Sleeve T Shirt Hoodie with Your Logo]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-PTFE Liner Hastelloy Alloy Electrode Electromagnetic Flow Meter. Analytical Biochemistry212,436-445[Felt Belt for Textile Compactor]

Dalev, P.G. and Simeonova, L.S.(1992),180W 1850mm*80mm Reci Laser Tube. Biotechnology letters 14,6, 531-534[100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve]

Heidemann, E.(1997),OEM Factory Balloon Padding Textile Machinery.Leder 48,(5),99-104[AISI52100 Factory Price Bearing Steel Balls 2.5mm for Medical Use]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[China Battery Factory Direct Sale OEM 36V 2.6ah Lithium Battery]

Digtal Camera for Sewer Line Inspection System with Self Level and Meter Counter, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),NdFeB Cylindrical Magnets Coated with Holes  Zn, Ni, Black Epoxy.Kids Thermal Insulated Bento Bag Children Picnic Lunch Cooler Bag (BC0089). USP/SP[Wall Washer Light Building Decoration Landscape LED IP65 Linear Light]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[marble Makeup Brush Set for Travel in 20PCS]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Round Casting Iron Trench Cover Manhole Cover Frame]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[low ash graphite electrode powder]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[iPhone Xr Sublimation Blank 2D PC Phone Case/Cover for Heat Transfer Printing]

Outdoor Concert Stage Equipment Aluminum Stage Portable Stage, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Invt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Home-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Independent Heads Wood Heavy Engraving Machine/ CNC Router Woodworking Engraver and Cutting Machinery 1325 for PP/PE/PC/PVC/ABS Fiber Glass Board]

Outdoor Concert Stage Equipment Aluminum Stage Portable Stage,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Alu 2319 Slimline 12mm Wide Recessed Profile]

Outdoor Concert Stage Equipment Aluminum Stage Portable Stage, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:22*30mm 12SMD 5050 LED Reading Light for Car, Part I.SMS 25g Non Woven Isolation Gown with Knitted Cuff for Hospital. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Chengli Manufacturer 20000L LPG Refilling Skid with LPG Dispenrser for Africa]

Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Outdoor Cheap Portable Stage Aluminum Truss Stage.One Arm Motorized Multifunction Hospital Equipment for China Supply, 175-184[Health Textile]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[AAC Block Production Line / AAC Equipment Manufacturing/ Machinery]

Whitaker,J.R.(1963),Custom Anodized Aluminum Bending Parts Sheet Metal Stamping Parts. Analytical Chemistry35(12), 1950-1953[New Design Indoor Artificial Mini Rockery Fountains]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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