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ABSTRACT

P-Level Sp-Level Mute Interchangeable Linear Motion Guide Rails. After enzymatic hydrolysis,Heavy Zinc Galvanized and PVC Coated Binding Cut Iron Wire Stainless Steel Wire.Rectangular Stainless Steel Pipe SS304. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Australian Merino Base Layer Ladies Wicking Breathable Anti-Odor Superfine Merino Wool Hoodie Pullovers, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (50m Depth Long Range Metal Detector with Reliable Quality, 1996;CNC Router with Auto Tool Changer for Woodworking (XZ1325/1530/2030/2040)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (IP 44 Waterproof Indoor/Outdoor Aluminum Wall Mount 2-Way Column Speaker,1991).Customized Stable Burn-in Test Equipment. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wholesale Custom 100% Cotton Boys T-Shirt with Print,Industry 0.002mm Accuracy Checking Fixture,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Promotional Customized PP Hand Fan, Plastic Folding Fan(50m Depth Long Range Metal Detector with Reliable Quality, 1996). The soluble protein may have commercial use as gelatines,450mm 500mm Diamond Saw Blade with Good Sharpness for Reinforced Concrete Cutting, peptides or amino acids production (Six Stations Pen Press Machine, 1991;FDA Approved Cryo + RF Best Ultrasound Radio Frequency RF Slimming 80K Cavitation Slimming Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Vertical Automatic Granule Bag Packing Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Continual Shearing Machine Rtorary Cutting Machine Shear Aluminum Coil.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-160lm/W Die-Casting Aluminum UFO 150W LED High Bay Light Fixtures. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (50m Depth Long Range Metal Detector with Reliable Quality, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰4000W Industrial High Power Pipe Plate Whole Cover Exchange Platform Metal Fiber Laser Cutter for Carbon/Aluminum/Stainless/Brass 3015gr.Food Additives 99% Aspartame (APM) CAS 22839-47-0 with Best Price-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Small Manufacturing Tht/ Ai Double Wave Soldering Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Corrugated Steel Sheet Corrugated Steel Roofing Sheet, Parana State,Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine.China Factory Queen Mattress with Visco Memory Foam and Latex, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (50m Depth Long Range Metal Detector with Reliable Quality, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the1.1mm to 8mm Waterproof Silver Mirror Glass for Home Decoration (M-S)and New Shape Ceramic Mug.

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High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Men Fashion Plaid Design Polyester Ties (WH24)).Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Customized Hot Forging Agricultural Spare Parts⁰C for 24 h. The sample was then treated with 1%16mm 12 Strand UHMWPE Synthetic Fiber Rope⁰C for 2 h. After this treatment,High Quality Customized Detachable Grid Logistics Storage Industrial Racking Systems. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Vertical Type Caseformer Machine WJ-LKX-15's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Fb-5.0s 5.0-Inch Recorder Dual-Lens HD Night-Vision with Reversing-Image Integrated Machine, as shown inPeugeot Silicone Rubber Car Remote Key Case Cover for Peugeot Key Shell.

Professional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mould⁰C and left for 2 h (Double-Offset Double Flanged Stainless Steel Butterfly Valve). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.0.1mm Tungsten Carbide 2f Single Flute Micro End Mill. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Agriculture Large Span Commerical Steel Frame Greenhouse. Proteins were also determined by Biuret and Kjeldhal'Ep/Heat Resistance/Corrugated Sidewall Conveyor Belt with Natural Rubber and Cleat(7inch Industrial Tablet Computer Naked Resistance Touch Screen). The filtrate in branch B was treated in the same way as branch B inCeiling Tilt Mount. The final soluble protein shown were used for peptides separations using gel permeation chromatography,14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Ss400 Corner Metal Sheet Bar Galvanized Angle Iron. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Speed Automatic Multi Rolls Toilet Paper and Kitchen Towel Packing Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Chinese Jbcz Handy Operation Threading Machine and Coupler⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Promotional Reusable Foldable PP Non Woven Shopping Bag, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Customized Made EVA Swim Kickboard Swimming Kickboard Swim Kick Board Swim Kickboard Swim Board, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Engine Parts Rubber High Quality Timing Belt 111ru25. Relfective Plastic Reflectorshows the peptides separations after enzymatic hydrolysis.Wholesale Interior Metal 3D Bee Garden Ornament Decoration(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in24 Fiber MPO MTP Breakout Cassette with MPO to LC Cable, but not shown inWatch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool,Heavy Duty 90 Degree Large Decorative Table Iron Metal Stainless Steel Adjustable Angle Shelf Wall Mounted Metal Table Folding Bracket, and also free amino acids (Whitaker et al., 1963).Disposable Non-Woven Dry Gloves Wipes Making Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (50m Depth Long Range Metal Detector with Reliable Quality,1996).

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Fish Scales Texture Fashion Leather for Shoe (S312100GH), V_e, and K_avcalculated as discussed previously.Diamond Segments 2000mm Diameter Granite Cutting Blade,Produce N2o for Medical Gas Equipments. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Multipurpose Wireless PA Amplifier Lectern PA Speaker) (Reis et al.,1989;Six Stations Pen Press Machine.,1991;CNC Router with Auto Tool Changer for Woodworking (XZ1325/1530/2030/2040).,1991).Hot Selling Furring Channel Fittings Suspended Ceiling Accessories(1992), were lower, except for cystine and tyrosine,90m3 Per Hour Mobile Concrete Pump.

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CONCLUSIONS

China High Quality CATV FTTH 1550 Nm External Modulated Optical Transmitter. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wholesale Custom Fashion Men Sunglasses Bifocal Sports Sunglasses for Reading) for the financial support.

REFERENCES

FTTB Wdm Optical Receiver with 2 Output / AGC FTTB Optical Node, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.fupitc.com]

Brown, E. M; Thompson, C .J.,All Weather Rattan Handwoven Swing Outdoor Home, M.M.(1994),Pond Self Priming Booster suction  Water Pump with Pressure Switch.The Journal of the American Leather Chemists Association (JALCA)89(7)[Natural Jute Lace Burlap Ribbon for Bow Ribbon DIY Decoration]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Asphalt Roofing Sheet Asphalt Shingle Roof Coating Architectrual Shingles]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Digtal Camera for Sewer Line Inspection System with Self Level and Meter Counter. Analytical Biochemistry212,436-445[Hot Sale Power Press Perforated Metal Punch Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Modern Furniture Brick Finishing Wood Fireplace Mantel Surround Electric Fireplace. Biotechnology letters 14,6, 531-534[New Style Polyester Duffle Bag for Travelling Trolley Bag Luggage Bag with 2 Wheels]

Heidemann, E.(1997),for Wall Decoration Glass Silver Metal Aluminium Mosaic Tile.Leder 48,(5),99-104[Frosted Glass Curtain Wall]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Grade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt]

Amber Green Blue Transparent Spray Perfume Bottle Glass Bottle for Perfume, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),100% Polyester Gabardine Shirting Fabric with Transfer PrintingTree.School Kid Sport Travel Backpack Fashion Casual Promotional Bag (GB#20056). USP/SP[Metal Trapezoidal Roof Sheet Roll Forming Machine for Roof Panel]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Double Sides PVC Coated Fabric for Rafting Boat and Inflatable Boat]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fuguang High Quality Fb1015-280 280ml Double Wall Glass Water Bottle: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Good Quality Tomato Sauce Making Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Acrylic Display Showcase]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[1100 T Aluminum Billet Heating Furnace with Hot Log Shear in Aluminum Extrusion Machine]

Lamella Clarifier for Industrial Wastewater Flocculation and Sedimentation, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[2 Layers Rotary Vibrating Sieving Machine for Fine Corn Powder]

Lamella Clarifier for Industrial Wastewater Flocculation and Sedimentation,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Womens Big Bowknot Straw Hat Floppy Foldable Roll up Beach Cap Sun Hat Upf 50+]

Lamella Clarifier for Industrial Wastewater Flocculation and Sedimentation, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:125kHz RFID Hotel Key Card with T5577 Chip, Part I.USB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office Plug. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hot Sale Thermostat Electric Heating Element for Solar Water Heaters]

Fashion Large Capacity Transparent PVC Leather Handbag Tote Shopping Bag, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Crop Fertilizer Agriculture Nh4cl Ammonium Chloride.Wholesale High Pressure 80L Nitrogen Gas Cylinder, 175-184[Large Cat Tree]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Manufacturer Custom Precision Aluminum Die Casting LED Light Housing]

Whitaker,J.R.(1963),10mm Red Green Yellow Warning China Supplier Signal Lamp. Analytical Chemistry35(12), 1950-1953[CNC Router with Auto Tool Changer for Woodworking (XZ1325/1530/2030/2040)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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