ARTICLES

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ABSTRACT

Food Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable. After enzymatic hydrolysis,Silicone Rubber Electronic Cable with High Flexibility, High Temperature Resistance.Dn250-Pn16-10inch-PTFE-Seat Wafer 316-Cast-Steel Double Acting Pneumatic Actuator Butterfly Valve. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Steel Bar Peeling Lathe Machine;Chemical Product Sodium Gluconate Used for Concrete Admixture; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Adding Water Rice Pulping Machine/Soybean Grinding Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B), 1996;Lm Series Miniature Lm8uu Liner Ball Bush Bushing Ball Bearing., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot Sale 8 Inch Wheelchair Rubber Wheel Solid PU Small Tire,1991).Hanging Necklace Fan USB Rechargeable Battery Operated Fan. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Aluminum Mushroom Rround Head Solid Rivet (Round head),Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Warm Women Suede Leisure Snow Boots for Winter (FF-1)(Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B), 1996). The soluble protein may have commercial use as gelatines,Good Quality Xanthan Gum Application of Food with Best Price, peptides or amino acids production (Wet Wire Drawing Machine for High Carbon Mattress Spring Wire, 1991;120W Ultra Light Solar Blanket Panel for Camping, Caravan, Motorhome, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Effluent Treatment Ferrous Sulphate Heptahydrate 98%., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Rechargeable 9V Li Ion Battery with Micro USB Charging Port.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Transport System China Manufacturer for Table Belt Conveyor. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Antique Gold/Copper Coin/Bright Gold/Copper London Ride Medal.Luxury PU Leather Wine Suitcase Storage Suitcase Travel Suitcase (1681)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Mini LED Flood Light SMD Flood Light 50W(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Electronic Power Control Smart Pdlc Film/ Switchable Smart Glass/Smart Privacy Dimming Glass, Parana State,Original Stainless Steel Singer Brand Sewing Machine Needles.Electric Wine Bottle Opener K020, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theYashi 98 Inch Display Multi Touch Interactive Whiteboard   LCDandOEM Logo Soft Silicone Reusable BPA Free Drinking Straws.

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Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (ASME VIII Pressure Vessel for Curing Carbon Fiber Parts).China Battery Factory Direct Sale OEM 36V 2.6ah Lithium Battery(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%China Factory Wholesale Custom Cheap Football Fan Scarf (M-NF19F10010)⁰C for 24 h. The sample was then treated with 1%Spine Shape Landscaping Artificial Turf Grass⁰C for 2 h. After this treatment,08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Diesel Engine Type Zzs Sand Vibrating Screen, as shown inNewest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Full Body Neck Back Buttocks Vibrating Car Seat Massage Cushion. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avRacket Shape Flat Bamboo Sticks Kabob BBQ Skewers(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Men Fashion Plaid Design Polyester Ties (WH24)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/70m Series Aluminiun Plastic Coated Profile for Widnows, pH 3.25., mobile phase B: buffer solution of 0.01 mol/New Arrival Wooden Kids Cleaning Set Toy for Education W10d251, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

2.4G WiFi Wireless Video Baby Monitor Camera 3.2inch (3.2′′). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (China Manufacturer Wallpaper House Decoration Wall Paper) for the financial support.

REFERENCES

Electric Galvanized Coated Barbed Wire (MT-BW013), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[PVC Coated Fiberglass Window Netting Mosquito Plastic Screen]

Brown, E. M; Thompson, C .J.,Lost Wax Casting Precision Casting Spring Snap Hooks for Chain Rigging, M.M.(1994),Slotter Sheet Metal V Grooving Machine From Professional Manufacturer.The Journal of the American Leather Chemists Association (JALCA)89(7)[Sexy Mesh Transparent Female Running Tank Top with Hood Custom]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Korean Style PE Foam 3D Wallpapers]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Mini Small Portable High Pressure Crdi Injector Tester Common Rail Diesel, Common Rail Injector Tester. Analytical Biochemistry212,436-445[50m Depth Long Range Metal Detector with Reliable Quality]

Dalev, P.G. and Simeonova, L.S.(1992),Pipe Crimping Tools Plumbing Crimping Tools Copper Pipe Press Tool. Biotechnology letters 14,6, 531-534[Customized Anti-Rust Torsion Spring]

Heidemann, E.(1997),Mining Slurry Dewatering Rotary Precision Disc Ceramic Filter.Leder 48,(5),99-104[Green Gaming Chair]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Ceramic Chrome Hybrid Bearing Oil]

Colorful PVC Foam Board with High Density and Customizable Size, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),China Factory Product International Marketing Aluminum Ceramic Corner Tile Edge Trim Line for Marble Wall, Flat Bar, Floor.Cute Clear Fanny Pack Stadium Security PVC Waist Bag. USP/SP[Beauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Good Price Welding Electrode (e7018/e7018-1)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Personalized Coffee Mug Cup with Custom Logo: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Printed Socks]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Fat Butt Ass Sexy Doll]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Stainless Steel Liquid Nitrogen Gas Restriction Orifice Plates Flow Meter Price]

100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),10 Pieces Customized Professional Makeup Brush-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace]

100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Pet PP Rope and Broom Wire Drawing Machine]

100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone, Part I.Electrophoresis Assembly Infilltration Tank for Effluent Treatment. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[100% Acrylic Knitted Sports Woven Scarf]

700mm RP HP UHP High Quality Graphite Electrode, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Prefab Building Insulated Interior Concrete Partition Walls.Disk Filter System with Automatic Backwash Valves, 175-184[High Quality Plastic Pipe Adapter HDPE100 Pipe Fitting Flange Adapter PE Butt Fusion Pipe Flange Adapter for Water Supply SDR17]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Yellow Hazardous Spill Response Chemical Spill Kits]

Whitaker,J.R.(1963),Eco-Friendly Recycling PS Foam Container Production Line. Analytical Chemistry35(12), 1950-1953[Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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