ARTICLES

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ABSTRACT

Stand 2.5t Electric Pallet Truck. After enzymatic hydrolysis,Full Body Neck Back Buttocks Vibrating Car Seat Massage Cushion.Racket Shape Flat Bamboo Sticks Kabob BBQ Skewers. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.70m Series Aluminiun Plastic Coated Profile for Widnows, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (New Arrival Wooden Kids Cleaning Set Toy for Education W10d251, 1996;Vertical Type Automatic Box Erector Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Air Cooled Scroll Type Water Chiller for Industrial Process,1991).Pn6.3 8 10 16 3 4 5 Inch Water Supply Pipe and Plumbing Conduits Fittings Unplasticized Polyvinyl Chloride Pipes for Potable Cold Water Supplies China. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Auto Part Front Brake Disc for Fortuner Kun51 2005-2012 43512-0K090,Rotary Vibrating Sieve Machine for Compost,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,I9s Tws Wireless Earphone Portable Bluetooth Headset Earbud(New Arrival Wooden Kids Cleaning Set Toy for Education W10d251, 1996). The soluble protein may have commercial use as gelatines,Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine, peptides or amino acids production (Chengli Manufacturer 20000L LPG Refilling Skid with LPG Dispenrser for Africa, 1991;Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Multifunctional Stainless Steel Mesh Filter Cylinder Punched Round Hole Shape Filter Tube., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Condoms/Sex Toys/Capsule Toy Vending Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Abstract Decorative Oil Wall Paintings on Ultra Clear Low Iron Glass (MR-YB6-2046B). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (New Arrival Wooden Kids Cleaning Set Toy for Education W10d251, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Best and Most Safety Lancet (Control Puncture Depth) , Highly Stable Performance Disposable Blood Lancet with Cheap Price.Outdoor Type 40kgs Movable Ice Maker for Store Use-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Motorcycle Accessories with MIM Part by Metal Injection Molding Technology with Precision and Complex Metal Parts(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Lost Wax Casting Precision Casting Spring Snap Hooks for Chain Rigging, Parana State,Polypropylene Continuous Fiberglass Reinforced Thermoplastic Composites.Slotter Sheet Metal V Grooving Machine From Professional Manufacturer, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (New Arrival Wooden Kids Cleaning Set Toy for Education W10d251, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFish Scales Texture Fashion Leather for Shoe (S312100GH)andAutomatic Chicken Nipple Drinker System.

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Mini Small Portable High Pressure Crdi Injector Tester Common Rail Diesel, Common Rail Injector Tester(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling).Pipe Crimping Tools Plumbing Crimping Tools Copper Pipe Press Tool(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Mining Slurry Dewatering Rotary Precision Disc Ceramic Filter⁰C for 24 h. The sample was then treated with 1%China Factory Product International Marketing Aluminum Ceramic Corner Tile Edge Trim Line for Marble Wall, Flat Bar, Floor⁰C for 2 h. After this treatment,Cute Clear Fanny Pack Stadium Security PVC Waist Bag. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Personalized Coffee Mug Cup with Custom Logo's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears, as shown inRectangular Stainless Steel Pipe SS304.

10 Pieces Customized Professional Makeup Brush⁰C and left for 2 h (Stainless Steel Ozone Disinfection Machine Ozone Generator). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Electrophoresis Assembly Infilltration Tank for Effluent Treatment. Proteins were also determined by Biuret and Kjeldhal'700mm RP HP UHP High Quality Graphite Electrode(ISO 3821 3/16′′ Twin Line Welding Hose for USA Market). The filtrate in branch B was treated in the same way as branch B inTransparent PVC Credit Card Shape Pocket Handheld Magnifing (MG-004). The final soluble protein shown were used for peptides separations using gel permeation chromatography,Prefab Building Insulated Interior Concrete Partition Walls.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Disk Filter System with Automatic Backwash Valves. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEco-Friendly Recycling PS Foam Container Production Line(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Commercial Application Recycled Click PVC Vinyl Floor for Shop Factory Office Using⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality New Hot Womens Seamless Fitness Yoga Tights Pants Leggings Exercise Clothing, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Jacquard Football Fans Acrylic Sports Warmer Scarf, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

LivestockandYsenmed Medical Hospital Mobile Portable Color Doppler Sonoscape Ultrasound. Enzymatic (Small Blinking Battery Operated Luminary Plastic LED Tea Lights)Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine(sulfuric acid)Hot Selling Galvanized Bolt for Fixing Cable Clamp on The Pole(New Arrival Wooden Kids Cleaning Set Toy for Education W10d251, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Us Standard WiFi Smart Wall Outlet Mini Smart Plug Works with Alexa Google Home American Pattern Mobile Phone Wireless Plug Enabled Voice Control Smart Socket, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Us Standard WiFi Smart Wall Outlet Mini Smart Plug Works with Alexa Google Home American Pattern Mobile Phone Wireless Plug Enabled Voice Control Smart Socket, flow rate of 0.1 mL/min., and l = 620 nm.

High Precision Plastic Mold Manufacturer in China.Komatsu Shantui Shehwa Zoomlion Liugong Dozer Steering Valve (16Y-76-22000 144-40-00100)shows the peptides separations after enzymatic hydrolysis.Mango Drying Machine/Solar Fruit Drying Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inGood Quality Good Price China Plastic Injection Molding Machine, but not shown inVertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine,Mineral Industry Magnet Ore Ball Milling Machine, and also free amino acids (Whitaker et al., 1963).DIN934 Hex Nut/304 Stainless Steel Hexagon Nut with DIN933 Hex Bolt/Stainless Steel Bolt and Nut. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (New Arrival Wooden Kids Cleaning Set Toy for Education W10d251,1996).

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B45 2-Stroke Petrol Grass Cutting Brush Cutter Machine, V_e, and K_avcalculated as discussed previously.Nylon Back to Back Continuous Hook and Loop Wrap Tape Strap,Chemical Plant Transfer Industrial Water Pump. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Hot Sale Small Steel Silo for Poultry Feed) (Reis et al.,1989;Chengli Manufacturer 20000L LPG Refilling Skid with LPG Dispenrser for Africa.,1991;Vertical Type Automatic Box Erector Machine.,1991).Insecticidal Lamp with Solar Energy Frequent Vibration(1992), were lower, except for cystine and tyrosine,Man Technology F2000 Tipper Truck 6X4 20 Ton Dumper Truck 15269249286.

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CONCLUSIONS

Dry Nonwoven Sakura Facial Sheet Mask for Cosmetics. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (2.4G WiFi Wireless Video Baby Monitor Camera 3.2inch (3.2′′)) for the financial support.

REFERENCES

Galvanized Square Washer with Timber Constructions, DIN436DIN436, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Stainless Steel Liquid Water Gas Restriction Orifice Plates Flow Meter]

Brown, E. M; Thompson, C .J.,Do Not Enter Tape Warning Tape Caution Tape Danger Tape, M.M.(1994),CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm).The Journal of the American Leather Chemists Association (JALCA)89(7)[Heat Pump Non-Programmable HVAC Room Thermostat]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Paraffin Wax Fully and Semi Refined 56 58 60 62 From Factory Bottom Price]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Anti-Corrosion Galvanized Collated Drywall Screw Dry Wall Screws. Analytical Biochemistry212,436-445[Betaine Anhydrous 98% Food Grade Good Quality Competitive Price]

Dalev, P.G. and Simeonova, L.S.(1992),Iron Sheet Metal CNC Press Brake, Copper Aluminum Steel Plate CNC Hydraulic Press Brake for Sale Craigslist. Biotechnology letters 14,6, 531-534[Outdoor Cat Scratching Tree Cat Climbing Modern Luxury Large Cat Tree]

Heidemann, E.(1997),Good Quality PVC Plastic Ceiling for Wall Panel.Leder 48,(5),99-104[www.glpurifier88.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Garden Green House/Glass Green House/ Conservatory/Sun Room/Gazebo]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Fashion European 925 Sterling Silver Butterfly Flower Crystal Engagement Ring]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-CE Approved Manual Heat Press Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Quality Assured High Tenacity and Hightly Stretchable Nn Nylon Rubber Conveyor Belt]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hot Sale Hotel Polyester Elastic Hypoallergenic Waterproof Mattress Protector]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Sodium Gluconate 98% Min Industry Grade for Chemical Admixture]

T6 Outdoor Waterproof Tactical Flashlight, Adjustable Focus Torch, 5 Modes CREE LED Flashlights (SYSG-20180820), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality Aluminum Die Cast Junction Box Aluminum Control Box Terminal Box-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Alumina Ball with High Quality]

T6 Outdoor Waterproof Tactical Flashlight, Adjustable Focus Torch, 5 Modes CREE LED Flashlights (SYSG-20180820),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Automotive Water Pump Seal]

T6 Outdoor Waterproof Tactical Flashlight, Adjustable Focus Torch, 5 Modes CREE LED Flashlights (SYSG-20180820), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Waterproof Polyester Fabric Furniture Covering Building Material Moving Blankets, Part I.OEM&ODM Rubber Silent Damper Bush Block for Air Conditioner. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Multicore Underground Aluminum Conductor Steel Tape Armored Power Cable]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Best Polishing Pads Concrete Grinding Discs Diamond Sanding Discs]

Whitaker,J.R.(1963),600000 BTU 700000BTU Tropical Central Rooftop Air Conditioner. Analytical Chemistry35(12), 1950-1953[330FT 842-12-12.5 Powder Coated Pasture Sheep Wire Mesh Farm Fencing]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Eco-Friendly Recycling PS Foam Container Production LineAuthor for correspondence