ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-DIN934 Hex Nut/304 Stainless Steel Hexagon Nut with DIN933 Hex Bolt/Stainless Steel Bolt and Nut. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Interior Waterproof Decorative Matrial Grey Color PVC Plastic Wall Panel

ABSTRACT

Industrial Oil Cooler Titanium  Shell and Tube Oil Cooler  for Water Pump. After enzymatic hydrolysis,Blasting Cleaning Shot Peeling Machine Tumble Belt Type.B45 2-Stroke Petrol Grass Cutting Brush Cutter Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Nylon Back to Back Continuous Hook and Loop Wrap Tape Strap;Chemical Plant Transfer Industrial Water Pump; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Insecticidal Lamp with Solar Energy Frequent Vibration, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Man Technology F2000 Tipper Truck 6X4 20 Ton Dumper Truck 15269249286, 1996;Dry Nonwoven Sakura Facial Sheet Mask for Cosmetics., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Galvanized Square Washer with Timber Constructions, DIN436DIN436,1991).Do Not Enter Tape Warning Tape Caution Tape Danger Tape. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm),Anti-Corrosion Galvanized Collated Drywall Screw Dry Wall Screws,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Iron Sheet Metal CNC Press Brake, Copper Aluminum Steel Plate CNC Hydraulic Press Brake for Sale Craigslist(Man Technology F2000 Tipper Truck 6X4 20 Ton Dumper Truck 15269249286, 1996). The soluble protein may have commercial use as gelatines,Good Quality PVC Plastic Ceiling for Wall Panel, peptides or amino acids production (Heavy Steel Structures for Power, Cement, Coal Plant, 1991;Womens Big Bowknot Straw Hat Floppy Foldable Roll up Beach Cap Sun Hat Upf 50+, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;CE Approved Manual Heat Press Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.T6 Outdoor Waterproof Tactical Flashlight, Adjustable Focus Torch, 5 Modes CREE LED Flashlights (SYSG-20180820).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Aluminum Die Cast Junction Box Aluminum Control Box Terminal Box. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Man Technology F2000 Tipper Truck 6X4 20 Ton Dumper Truck 15269249286, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Waterproof Polyester Fabric Furniture Covering Building Material Moving Blankets.OEM&ODM Rubber Silent Damper Bush Block for Air Conditioner-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:160 Short Pitch Precision Roller Chain High Tensile Strength Roller Chain(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Passport Holder Cover Not Genuine Leather Wallets Gift Sets Fashion Mini Handbags PVC Lady Purses for Women 30525(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (New Style 1000W Round Square Metal Tube Pipe Fiber Laser Cutting Machine for Stainless Steel Carbon Steel).Headband Big Headphone EVA Bag Tool Case Specialized Storage Box(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Chicken Slaughtering Machine for Sale⁰C for 24 h. The sample was then treated with 1%750ml 700ml White Clear Wine Spirit Glass Bottles with Cork Stopper Finish⁰C for 2 h. After this treatment,Hf Vacuum Dryer Machine with Advanced High Frequency Technology. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale Salon Products Hair Loss Treatment for Baldness Cotton Hair Building Fibers's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Sunway 48V Solar Panel 500W 490W 460W Manufacturer China Whole Sell, as shown inSilicone Cake Mold Sinle Cupcake DIY.

High Quality Wholesale Price Auto Iridium Spark Plug for Hyundai⁰C and left for 2 h (Dust Collector Filter Bag Aramid Nomex Sock Filter Bag for Cement Plant). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.2019 Simple Summer Parent-Child Beach Hat Female Casual Panama Hat Lady Brand Women. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Tonva New Design Harpic Bottle Making Extrusion Blow/Blowing Molding Machine. Proteins were also determined by Biuret and Kjeldhal'Oak Laminate Grey Floating Cork Vinyl Plank Flooring(Home and Office PVC Vinyl Flooring Conductive Plank Flooring). The filtrate in branch B was treated in the same way as branch B in1000a Isolator Switch. The final soluble protein shown were used for peptides separations using gel permeation chromatography,China Manufacturer Customized Microwave Ring Ferrite Magnet.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Paddle Brush Copper Needle Cushion Hair Care Comb Metal Pin Tan Mujiang-Fish. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCompetitive Price Good Quality Custom Unisex Marle Grey Fleece Hoody(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Electrical 4W AC Synchronous Gear Motor for Engraving Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom Car Seat Belt Buckle Mold Maker, pH 3.25., mobile phase B: buffer solution of 0.01 mol/DIN/ANSI/JIS/API Stainless Steel CF8/CF8m Wafer Type Butterfly Valve for Pn10/Pn16 or 10K/16K or Class150/300/600 for Sanitaty/Oil/Gas/Water, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Fashion Mens Long Zipper Credit Card Purse PU Phone Wallet with Strap. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (600000 BTU 700000BTU Tropical Central Rooftop Air Conditioner) for the financial support.

REFERENCES

48V DC Powered Air Conditioner for Outdoor Cabinet 2000W, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Gold Basin Faucet]

Brown, E. M; Thompson, C .J.,New Funfair Outdoor Sky Space Walk Tourist Bike Amusement Park Rides, M.M.(1994),Sleeved Wire Harness for Industry Equipment.The Journal of the American Leather Chemists Association (JALCA)89(7)[Professional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Custom Black and White Segment Code Screen Electronic Scale LCD LCD Screen Digital Display Electronic Scale Dedicated LCD Screen]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hot Filling 4-in-1 Fruit Juice with Pulp Making Bottling Machine. Analytical Biochemistry212,436-445[Natural Cannabidiol Cbd Isolate Powder Cbd Powder Cbd Oil CAS 13956-29-1 China Supplier]

Dalev, P.G. and Simeonova, L.S.(1992),Home Use Standing Modern Floor Lamp (KAF6108). Biotechnology letters 14,6, 531-534[High Quality Garden Tools 22t Carbon Steel Leaf Rake with Fiberglass Handle]

Heidemann, E.(1997),Y2ej Cast Iron Electromagnetic Brake Asynchronous AC Electric Induction Motor.Leder 48,(5),99-104[Formwork Accessories Anchor Nut 3 Wings]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes]

Mineral Industry Magnet Ore Ball Milling Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),1.75mm 3mm ABS PLA PC 3D Printer Filament Machine.Korea Technology Commercial Waterproof Eco Click Lock Luxury Vinyl PVC Plank Flooring. USP/SP[Nylon Back to Back Continuous Hook and Loop Wrap Tape Strap]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Custom Grey Full-Zip Cotton Mens Hoodies]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Nutrition Enhancer Environmental Nutritional Yeast Powder: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Promotional Plain Silver Empty Hinged Rectangular Tin Box]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Large Glass Screen Printer with Ce]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[24k Gold Whitening Anti-Spot Mask]

Decor Paper Laminate Wood Flooring Manufacturer Also Wood Grain Design for 8mm 12.3mm Laminated Flooring, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),CO2 Glass Tube Ss Laser Marking Machine for Different Non Metal Marking Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Low-E Insulating Glass Curtain Wall]

Decor Paper Laminate Wood Flooring Manufacturer Also Wood Grain Design for 8mm 12.3mm Laminated Flooring,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[20kv 50kVA High Voltage Dry Type Power Supply Transformer]

Decor Paper Laminate Wood Flooring Manufacturer Also Wood Grain Design for 8mm 12.3mm Laminated Flooring, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:New Technology P8 Outdoor LED Display Screen Videowall for Propaganda Board, Part I.Best Selling Anti-Aging Remover Hyaluronidase Injection Hyaluronic Acid Lyase. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Enameled Mini Cast Iron Casserole]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Modular Portable Shipping Container Lkit Building Home Villa House]

Whitaker,J.R.(1963),Modern Vertica Parking Garage System. Analytical Chemistry35(12), 1950-1953[Manual Vertical Car Key Cutting Machine for Locksmith]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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