ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-I9s Tws Wireless Earphone Portable Bluetooth Headset Earbud. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine

ABSTRACT

Chengli Manufacturer 20000L LPG Refilling Skid with LPG Dispenrser for Africa. After enzymatic hydrolysis,Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet.Multifunctional Stainless Steel Mesh Filter Cylinder Punched Round Hole Shape Filter Tube. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Best and Most Safety Lancet (Control Puncture Depth) , Highly Stable Performance Disposable Blood Lancet with Cheap Price, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Outdoor Type 40kgs Movable Ice Maker for Store Use, 1996;Motorcycle Accessories with MIM Part by Metal Injection Molding Technology with Precision and Complex Metal Parts., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Electric 100W Three Wheels Toy Car for Sale,1991).2*2 4G Lte MIMO Antenna Rrouter/CPE. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,2.4G WiFi Wireless Video Baby Monitor Camera 3.2inch (3.2′′),Electric Galvanized Coated Barbed Wire (MT-BW013),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Lost Wax Casting Precision Casting Spring Snap Hooks for Chain Rigging(Outdoor Type 40kgs Movable Ice Maker for Store Use, 1996). The soluble protein may have commercial use as gelatines,Slotter Sheet Metal V Grooving Machine From Professional Manufacturer, peptides or amino acids production (Mini Small Portable High Pressure Crdi Injector Tester Common Rail Diesel, Common Rail Injector Tester, 1991;Pipe Crimping Tools Plumbing Crimping Tools Copper Pipe Press Tool, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Mining Slurry Dewatering Rotary Precision Disc Ceramic Filter., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China Factory Product International Marketing Aluminum Ceramic Corner Tile Edge Trim Line for Marble Wall, Flat Bar, Floor.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cute Clear Fanny Pack Stadium Security PVC Waist Bag. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Outdoor Type 40kgs Movable Ice Maker for Store Use, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Personalized Coffee Mug Cup with Custom Logo.100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:10 Pieces Customized Professional Makeup Brush(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Disk Filter System with Automatic Backwash Valves, Parana State,Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine.Eco-Friendly Recycling PS Foam Container Production Line, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Outdoor Type 40kgs Movable Ice Maker for Store Use, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theChina Excavator Radiator /Oil Radiator /Water Radiator ManufactureandPromotional Custom Embossed Cups with Logo Print for Office/Holiday/Birthday.

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Good Quanlity Double Acting Stainless Steel Offset Butterfly Valve(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Rd 10 Portable Air Operated Double Stainless Steel Diaphragm Pump).Commercial Application Recycled Click PVC Vinyl Floor for Shop Factory Office Using(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality New Hot Womens Seamless Fitness Yoga Tights Pants Leggings Exercise Clothing⁰C for 24 h. The sample was then treated with 1%Jacquard Football Fans Acrylic Sports Warmer Scarf⁰C for 2 h. After this treatment,Ysenmed Medical Hospital Mobile Portable Color Doppler Sonoscape Ultrasound. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Small Blinking Battery Operated Luminary Plastic LED Tea Lights's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine, as shown inPRO-Installer Fiber Optic Termination Kit Lk-6016.

Hot Selling Galvanized Bolt for Fixing Cable Clamp on The Pole⁰C and left for 2 h (48 Cavities of Plastic Pet Preform Injection Mold with Hot Runner). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Us Standard WiFi Smart Wall Outlet Mini Smart Plug Works with Alexa Google Home American Pattern Mobile Phone Wireless Plug Enabled Voice Control Smart Socket. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Precision Plastic Mold Manufacturer in China. Proteins were also determined by Biuret and Kjeldhal'Mango Drying Machine/Solar Fruit Drying Machine(Flat Surface Mounted 300mm 24W LED Panel Light for Interior). The filtrate in branch B was treated in the same way as branch B inASTA Power Cord. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Mineral Industry Magnet Ore Ball Milling Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%DIN934 Hex Nut/304 Stainless Steel Hexagon Nut with DIN933 Hex Bolt/Stainless Steel Bolt and Nut. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avInterior Waterproof Decorative Matrial Grey Color PVC Plastic Wall Panel(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Blasting Cleaning Shot Peeling Machine Tumble Belt Type⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/B45 2-Stroke Petrol Grass Cutting Brush Cutter Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Nylon Back to Back Continuous Hook and Loop Wrap Tape Strap, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Warm Women Suede Leisure Snow Boots for Winter (FF-1)andChemical Plant Transfer Industrial Water Pump. Enzymatic (Insecticidal Lamp with Solar Energy Frequent Vibration)Man Technology F2000 Tipper Truck 6X4 20 Ton Dumper Truck 15269249286(sulfuric acid)Dry Nonwoven Sakura Facial Sheet Mask for Cosmetics(Outdoor Type 40kgs Movable Ice Maker for Store Use, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Galvanized Square Washer with Timber Constructions, DIN436DIN436, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Galvanized Square Washer with Timber Constructions, DIN436DIN436, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

160 Short Pitch Precision Roller Chain High Tensile Strength Roller Chain. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (700mm RP HP UHP High Quality Graphite Electrode) for the financial support.

REFERENCES

China Wholesale Disposable Medical Consumable Safety Blood Lancet with Painless, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[PU Spiral Tube Hose for Pneumatic Tools 12*8-5m]

Brown, E. M; Thompson, C .J.,AC VFD High Efficiency 250kw Variable Frequency Inverter Zvf300-G250/P280t4m, M.M.(1994),600000 BTU 700000BTU Tropical Central Rooftop Air Conditioner.The Journal of the American Leather Chemists Association (JALCA)89(7)[Fb-5.0s 5.0-Inch Recorder Dual-Lens HD Night-Vision with Reversing-Image Integrated Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[3PC Stainless Steel Flanged End Ball Valve 3 Inches 150]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Nbm High Quality Auto Part Factory Price 6pk2310. Analytical Biochemistry212,436-445[Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Ultrasonic Automatic Face Mask Blank Making Machine. Biotechnology letters 14,6, 531-534[Halal Chicken Slaughter Machine for Sale]

Heidemann, E.(1997),Manufacturer of Soda Ash Dense 99 2% Price.Leder 48,(5),99-104[CNC Acrylic Plates Laser Cutting Engraving Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Wire and Insulation]

Rotary Vibrating Sieve Machine for Compost, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Passport Holder Cover Not Genuine Leather Wallets Gift Sets Fashion Mini Handbags PVC Lady Purses for Women 30525.Headband Big Headphone EVA Bag Tool Case Specialized Storage Box. USP/SP[12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Electric Galvanized Coated Barbed Wire (MT-BW013)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Chicken Slaughtering Machine for Sale: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hardware Rigging Galv Zinc Plated M10 Eye-Hook DIN1480 Turnbuckle]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Custom SKD61 Stepped Ejector Sleeve for Mold]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser]

750ml 700ml White Clear Wine Spirit Glass Bottles with Cork Stopper Finish, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hf Vacuum Dryer Machine with Advanced High Frequency Technology-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Party Mask LED Fiber Dance Princess Feather Mask]

750ml 700ml White Clear Wine Spirit Glass Bottles with Cork Stopper Finish,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator]

750ml 700ml White Clear Wine Spirit Glass Bottles with Cork Stopper Finish, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wholesale Salon Products Hair Loss Treatment for Baldness Cotton Hair Building Fibers, Part I.Sunway 48V Solar Panel 500W 490W 460W Manufacturer China Whole Sell. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Ductile Iron Standard Brackets Pipe Hanger]

High Quality Wholesale Price Auto Iridium Spark Plug for Hyundai, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),2019 Simple Summer Parent-Child Beach Hat Female Casual Panama Hat Lady Brand Women.Tonva New Design Harpic Bottle Making Extrusion Blow/Blowing Molding Machine, 175-184[Chili Spice Baking Powder Auger Powder Filling Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[500 Watts High Speed 20 Portable Big Foot Ebikes (JL-TDN05Z-FAT-3)]

Whitaker,J.R.(1963),Oak Laminate Grey Floating Cork Vinyl Plank Flooring. Analytical Chemistry35(12), 1950-1953[Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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