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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Aluminum Low Profile Adjustable Bracket. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Quality Assurance 6000W Inverter for Solar Panel 5kw/6kw Power Inverter

ABSTRACT

High Quality Acrylic Beer Display Racks with LED. After enzymatic hydrolysis,Blank Personalized Engraved Plaques Name Metal Art Crafts Plating Copper 3D Marathon Sports Medal with Customized Design (MD04-C).Baby Feeding Chair Plastic Chair for Feeding Hebei China. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Round Type Twin Screw Extruder Barrel Manufacturer, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (ASA-UPVC Anti-Corrosive Composite Roof Tile, 1996;Fabricated Steel and Heavy-Duty Sheet Weigh Bench for Ventilation System., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Sexy Mesh Transparent Female Running Tank Top with Hood Custom,1991).Color Cardstock Color Paper Manufacturer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Custom High Precision Aluminum/Stainless Steel/Brass CNC Machining Car Spare Parts,Home and Office PVC Vinyl Flooring Conductive Plank Flooring,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Precision Engineering Parts Bearing Support Unit for Ball Screw(ASA-UPVC Anti-Corrosive Composite Roof Tile, 1996). The soluble protein may have commercial use as gelatines,LED Table Lamp LED Desk Lamps LED Table Light 5W DC5V 1200mAh Modern Fashion LED Baby Study Light Lighting Eye Protection, peptides or amino acids production (165cm Realistic Full Solid TPE Sex Doll Big Breast Love Doll for Man, 1991;Silicone Cake Mold Sinle Cupcake DIY, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Plastic Pipe Clamp for Refrigeration Pipe System Pipe Fitting., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.10g Food/Pharmaceutical Desiccant Silica Gel for Moisture Absorbing.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cross Stripe Sleeveless Yoga Wear. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (ASA-UPVC Anti-Corrosive Composite Roof Tile, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Construction & Decoration ERW Square Steel Tube Manufacturer.DVD Audio RCA Wiring Harness Cable for DVD Pioneer Avh-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Black Steel ASTM A192 Seamless Pipe Sch120(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

CNC Machining Checking Auto Car Checking Fixture, Parana State,Quality Assurance 6000W Inverter for Solar Panel 5kw/6kw Power Inverter.Stainless Steel Thread Ends Sanitary Butterfly Valve with Pull Handle, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (ASA-UPVC Anti-Corrosive Composite Roof Tile, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Aluminum Insulating Ceramic Fiber Paper for Industrial KilnsandFactory Price 6mm Stainless Steel Wire Rope 7*7.

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T Series Bevel Helical Gearboxes for Sale(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (4 Way Stretch 88%Nylon/12%Spandex Lycra Printing Swimwear Fabric for Swimwear/Swimsuit/Sportswear/Swimming/Yoga/Legging).Military Police Army Outdoor Protective Safety Bulletproof Camouflage Protective Vest(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Colorful PVC Foam Board with High Density and Customizable Size⁰C for 24 h. The sample was then treated with 1%Air Vent Diffuser Adjustbable Air Well and Jet Diffuser⁰C for 2 h. After this treatment,Original Stainless Steel Singer Brand Sewing Machine Needles. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Food Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Silicone Rubber Electronic Cable with High Flexibility, High Temperature Resistance, as shown inGlass Food Jar & Plastic Lid.

Dn250-Pn16-10inch-PTFE-Seat Wafer 316-Cast-Steel Double Acting Pneumatic Actuator Butterfly Valve⁰C and left for 2 h (NPK 15 15 15 Compound Fertilizer at a Hot Selling). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Steel Bar Peeling Lathe Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Chemical Product Sodium Gluconate Used for Concrete Admixture. Proteins were also determined by Biuret and Kjeldhal'Adding Water Rice Pulping Machine/Soybean Grinding Machine(Prefab Steel Structure Shed for Storage Warehouse). The filtrate in branch B was treated in the same way as branch B inwww.nq-chem.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Lm Series Miniature Lm8uu Liner Ball Bush Bushing Ball Bearing. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHot Sale 8 Inch Wheelchair Rubber Wheel Solid PU Small Tire(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Aluminum Mushroom Rround Head Solid Rivet (Round head)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Warm Women Suede Leisure Snow Boots for Winter (FF-1), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

China Factory Wholesale Custom Cheap Football Fan Scarf (M-NF19F10010). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (BOPP CPP Laminate Food Packaging Metalized Pet Cup Sealing Film) for the financial support.

REFERENCES

Spine Shape Landscaping Artificial Turf Grass, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Solid Empty Pill Bottle]

Brown, E. M; Thompson, C .J.,08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain, M.M.(1994),3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board.The Journal of the American Leather Chemists Association (JALCA)89(7)[22 Inch Wall Mountdigital Photo Frame Interactive Touch Screen Bus Media Player Advertising Poster]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Diesel Engine Type Zzs Sand Vibrating Screen. Analytical Biochemistry212,436-445[Pen Type pH Meter pH-031&0.00-14.00pH Tester]

Dalev, P.G. and Simeonova, L.S.(1992),Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply. Biotechnology letters 14,6, 531-534[Stamping Metal Parts Manufacturer Sheet Parts Fabrication]

Heidemann, E.(1997),Bluetooth Smart Watch with Heart Rate Monitor for Men 72087.Leder 48,(5),99-104[Floating Tilapia Feed Twin Screw Extruder Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wooden Heavy Door All Kind of Stainless Steel Ball Bearing Door Hinge]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Efficiency Solar Cell Photovoltaic 300W Hot Sell]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Stand 2.5t Electric Pallet Truck: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Tc90 Crosslinked Polyethylene Insulated Aluminum Alloy Cable Made in China]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Outdoor CATV Optical Receiver Node]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Powder Coating Polyester Resin with Factory Price]

Full Body Neck Back Buttocks Vibrating Car Seat Massage Cushion, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Racket Shape Flat Bamboo Sticks Kabob BBQ Skewers-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60]

Full Body Neck Back Buttocks Vibrating Car Seat Massage Cushion,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444)]

Full Body Neck Back Buttocks Vibrating Car Seat Massage Cushion, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Automatic Round Bottle Shrink Sleeve Labeling Machine for PVC Label, Part I.Men Fashion Plaid Design Polyester Ties (WH24). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display]

70m Series Aluminiun Plastic Coated Profile for Widnows, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),New Arrival Wooden Kids Cleaning Set Toy for Education W10d251.Vertical Type Automatic Box Erector Machine, 175-184[FSTN LCD Graphic Module Cog White Characters on Blue Background FPC Module]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Pond Self Priming Booster suction  Water Pump with Pressure Switch]

Whitaker,J.R.(1963),Air Cooled Scroll Type Water Chiller for Industrial Process. Analytical Chemistry35(12), 1950-1953[Flexible Magnetic Rubber Sheet Soft Magnetic Tape]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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