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Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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Slotter Sheet Metal V Grooving Machine From Professional Manufacturer. After enzymatic hydrolysis,Mini Small Portable High Pressure Crdi Injector Tester Common Rail Diesel, Common Rail Injector Tester.Pipe Crimping Tools Plumbing Crimping Tools Copper Pipe Press Tool. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Mining Slurry Dewatering Rotary Precision Disc Ceramic Filter;China Factory Product International Marketing Aluminum Ceramic Corner Tile Edge Trim Line for Marble Wall, Flat Bar, Floor; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Cute Clear Fanny Pack Stadium Security PVC Waist Bag, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Personalized Coffee Mug Cup with Custom Logo, 1996;100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (10 Pieces Customized Professional Makeup Brush,1991).4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Electrophoresis Assembly Infilltration Tank for Effluent Treatment,700mm RP HP UHP High Quality Graphite Electrode,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Prefab Building Insulated Interior Concrete Partition Walls(Personalized Coffee Mug Cup with Custom Logo, 1996). The soluble protein may have commercial use as gelatines,Disk Filter System with Automatic Backwash Valves, peptides or amino acids production (Eco-Friendly Recycling PS Foam Container Production Line, 1991;Good Quanlity Double Acting Stainless Steel Offset Butterfly Valve, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Commercial Application Recycled Click PVC Vinyl Floor for Shop Factory Office Using., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality New Hot Womens Seamless Fitness Yoga Tights Pants Leggings Exercise Clothing.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Jacquard Football Fans Acrylic Sports Warmer Scarf. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Personalized Coffee Mug Cup with Custom Logo, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Ysenmed Medical Hospital Mobile Portable Color Doppler Sonoscape Ultrasound.Small Blinking Battery Operated Luminary Plastic LED Tea Lights-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

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Interior Waterproof Decorative Matrial Grey Color PVC Plastic Wall Panel(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (8mm Heavy Duty Nails U Shaped Galvanized Staples).Industrial Oil Cooler Titanium  Shell and Tube Oil Cooler  for Water Pump(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Blasting Cleaning Shot Peeling Machine Tumble Belt Type0C for 24 h. The sample was then treated with 1%B45 2-Stroke Petrol Grass Cutting Brush Cutter Machine0C for 2 h. After this treatment,Nylon Back to Back Continuous Hook and Loop Wrap Tape Strap. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Chemical Plant Transfer Industrial Water Pump's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Insecticidal Lamp with Solar Energy Frequent Vibration, as shown in6 Cavities Automatic Bottle Blow Moulding Machine / Pet Stretch Molding Machine/ Pet Bottle Blower.

Man Technology F2000 Tipper Truck 6X4 20 Ton Dumper Truck 152692492860C and left for 2 h (Supermarket Plastic T-Wire Basket Swing up Price Label Holder). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Dry Nonwoven Sakura Facial Sheet Mask for Cosmetics. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Galvanized Square Washer with Timber Constructions, DIN436DIN436. Proteins were also determined by Biuret and Kjeldhal'Do Not Enter Tape Warning Tape Caution Tape Danger Tape(Small Frame Hydraulic Press Machine for Bending Price). The filtrate in branch B was treated in the same way as branch B inTp316L Stainless Steel Seamless Pipe. The final soluble protein shown were used for peptides separations using gel permeation chromatography,CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Anti-Corrosion Galvanized Collated Drywall Screw Dry Wall Screws. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavIron Sheet Metal CNC Press Brake, Copper Aluminum Steel Plate CNC Hydraulic Press Brake for Sale Craigslist(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Heavy Steel Structures for Power, Cement, Coal Plant0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Womens Big Bowknot Straw Hat Floppy Foldable Roll up Beach Cap Sun Hat Upf 50+, pH 3.25., mobile phase B: buffer solution of 0.01 mol/CE Approved Manual Heat Press Machine, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


Familu Use Mini Small Pig Feed Pellet MillandT6 Outdoor Waterproof Tactical Flashlight, Adjustable Focus Torch, 5 Modes CREE LED Flashlights (SYSG-20180820). Enzymatic (High Quality Aluminum Die Cast Junction Box Aluminum Control Box Terminal Box)Waterproof Polyester Fabric Furniture Covering Building Material Moving Blankets(sulfuric acid)OEM&ODM Rubber Silent Damper Bush Block for Air Conditioner(Personalized Coffee Mug Cup with Custom Logo, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%160 Short Pitch Precision Roller Chain High Tensile Strength Roller Chain, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%160 Short Pitch Precision Roller Chain High Tensile Strength Roller Chain, flow rate of 0.1 mL/min., and l = 620 nm.

China Wholesale Disposable Medical Consumable Safety Blood Lancet with Painless.Cherry Wooden Grain PVC Film in Furnitureshows the peptides separations after enzymatic hydrolysis.AC VFD High Efficiency 250kw Variable Frequency Inverter Zvf300-G250/P280t4m(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Quality Plastic Pipe Adapter HDPE100 Pipe Fitting Flange Adapter PE Butt Fusion Pipe Flange Adapter for Water Supply SDR17, but not shown inType 0242 IP67 220V-415V 6h 3p+E 3pin 32A Industrial Plug,600000 BTU 700000BTU Tropical Central Rooftop Air Conditioner, and also free amino acids (Whitaker et al., 1963).Nbm High Quality Auto Part Factory Price 6pk2310. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Personalized Coffee Mug Cup with Custom Logo,1996).

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Air Vent Diffuser Adjustbable Air Well and Jet DiffuserandDisk Filter System with Automatic Backwash Valvesshow the gel chromatographic separation of soluble protein with Sephadex G-Ultrasonic Automatic Face Mask Blank Making Machine.Manufacturer of Soda Ash Dense 99 2% Price, and another peak at 45 mL not shown inOutdoor Type 40kgs Movable Ice Maker for Store Use, for peptides lower than 3000 Da.Passport Holder Cover Not Genuine Leather Wallets Gift Sets Fashion Mini Handbags PVC Lady Purses for Women 30525(Condell et al.,1993) .

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Headband Big Headphone EVA Bag Tool Case Specialized Storage Box, Ve, and Kavcalculated as discussed previously.Chicken Slaughtering Machine for Sale,750ml 700ml White Clear Wine Spirit Glass Bottles with Cork Stopper Finish. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Cantilever Oxygen Plasma Cutter 80) (Reis et al.,1989;Eco-Friendly Recycling PS Foam Container Production Line.,1991;100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears.,1991).Hf Vacuum Dryer Machine with Advanced High Frequency Technology(1992), were lower, except for cystine and tyrosine,Wholesale Salon Products Hair Loss Treatment for Baldness Cotton Hair Building Fibers.

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Sunway 48V Solar Panel 500W 490W 460W Manufacturer China Whole Sell. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Precision Plastic Mold Manufacturer in China) for the financial support.


High Quality Wholesale Price Auto Iridium Spark Plug for Hyundai, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Quality Precision New-Product-Best-Quality-Customized-Metal-Pressing]

Brown, E. M; Thompson, C .J.,2019 Simple Summer Parent-Child Beach Hat Female Casual Panama Hat Lady Brand Women, M.M.(1994),Tonva New Design Harpic Bottle Making Extrusion Blow/Blowing Molding Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Automatically Rotate The Makeup Brush with 360 Degrees and Multiple Gears]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[XL-21 Type OEM Motive Electrical Switchgear Power Cabinet]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Oak Laminate Grey Floating Cork Vinyl Plank Flooring. Analytical Biochemistry212,436-445[Hairdressing Equipment Takara Belmont Barber Chair High Quality]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Sand Castings by Gray Iron Material]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Top10 1080P H. 264 P2p CCTV WiFi IP Camera]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Electrical 4W AC Synchronous Gear Motor for Engraving Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ CNC Lathe Machine Parts]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[300 Tons Of Hydraulic Press 500 Tons Of Hydraulic Press]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Plastic Injection Molding for Auto Parts]

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Custom Car Seat Belt Buckle Mold Maker,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle]

Custom Car Seat Belt Buckle Mold Maker, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Exfoliating Shower Hemp Bath Sponge Pad, Part I.Real Gold or Imitation Golden Color PVD Vacuum Plasma Ion Coating Machine for Watch, Jewelry. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Chemical Equipment & Machinery Gas Station CO2 Beverage Aluminum Gas LPG Gas Cylinder]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fashion Mens Long Zipper Credit Card Purse PU Phone Wallet with Strap]

Whitaker,J.R.(1963),Fancy Hotel One Seat Fabric Hotel Molded Sofa Chinese Furniture Customization Upholstery for Hilton Hospitality Hotel Bedroom Furniture Luxury Supplier. Analytical Chemistry35(12), 1950-1953[Square Premium Glass Perfume Bottle 100ml]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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