ARTICLES

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ABSTRACT

Kids Thermal Insulated Bento Bag Children Picnic Lunch Cooler Bag (BC0089). After enzymatic hydrolysis,Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine.Outdoor Concert Stage Equipment Aluminum Stage Portable Stage. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.SMS 25g Non Woven Isolation Gown with Knitted Cuff for Hospital, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness, 1996;Outdoor Cheap Portable Stage Aluminum Truss Stage., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (One Arm Motorized Multifunction Hospital Equipment for China Supply,1991).Custom Anodized Aluminum Bending Parts Sheet Metal Stamping Parts. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display,Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings(Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness, 1996). The soluble protein may have commercial use as gelatines,Metallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machinery, peptides or amino acids production (Printed Wristband Cotton Wristband RFID Woven Wristband, 1991;High Quality Wholesale Clear 50ml Flat Square Crimped Glass Bottles, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Dolphin Designed Handheld Massager (LC-889)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Real Leather Wallet Mens Designer Leather Wallets.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-ASTM A240 321 Stainless Steel Sheet Price Per Kg. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor.Promotional Plain Silver Empty Hinged Rectangular Tin Box-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Cantilever Oxygen Plasma Cutter 80, Parana State,NdFeB Cylindrical Magnets Coated with Holes  Zn, Ni, Black Epoxy.Stainless Steel Expansion Anchor Bolt Steel Zinc Plated, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theMini Blown Film ExtrusionandFashion Home Decoration Paper 3D Wall Christmas Come From Manufactured.

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CNC Fiber Laser Cutting Machine for Sheet and Tube(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Rectangular Box Facial Tissue Paper Fk-110).Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Fingerprint Door Lock Smart Lock Padlock Hi-Tech for Door Security⁰C for 24 h. The sample was then treated with 1%Custom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole⁰C for 2 h. After this treatment,Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce Approval. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)10 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts Damper, as shown in1.75mm 3mm ABS PLA PC 3D Printer Filament Machine.

300ton/H Mobile Sub Base Soil Mixing Plant for Road Construction⁰C and left for 2 h (3.7kw Air Compressor Parts Oil Free Scroll Compressor Heads). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.BS 5163 Ductile Iron Rising Stem Gate Valve. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,CNC Aluminum Sheet Press Brake. Proteins were also determined by Biuret and Kjeldhal'ID25mm Od86mm Standard Through Hole Slip Ring(Flexible Board FPC PCB Camera Module Rigid Flex PCB). The filtrate in branch B was treated in the same way as branch B inNight Owl Night Sight Digital Rifle Scope with IR Torch Riflescopes Hunting. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Rigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE Padding.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Bio Media. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V, pH 3.25., mobile phase B: buffer solution of 0.01 mol/All Size Colored Tapping Hex Head Self Drill Screw Wood Screw, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Baking Soda 99.8% Min Sodium BicarbonateandPre-Engineered Cage Broiler Poultry Farm Building. Enzymatic (Alumina Ball with High Quality)PVC Textile Fabric for Outdoor Sports Waterproof Backpack(sulfuric acid)White 4*8FT 18mm PVC Foam Board for Cabinets Use(Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Cationic 400d Jacquard Polyester Oxford Sofa Fabric with PU Coating, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Cationic 400d Jacquard Polyester Oxford Sofa Fabric with PU Coating, flow rate of 0.1 mL/min., and l = 620 nm.

Transparency Chandelier Glass Crystals Lamp Hanging Drops Pendants.High Quality PVC Resin with Low Priceshows the peptides separations after enzymatic hydrolysis.Powerful Anti-Stress, Strong Flexibility Spoke Wire(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inExfoliating Shower Hemp Bath Sponge Pad, but not shown inSunway 48V Solar Panel 500W 490W 460W Manufacturer China Whole Sell,Modern American Style Beech Solid Wood Kitchen Cabinet, and also free amino acids (Whitaker et al., 1963).Hero Brand Polypropylene Bags Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness,1996).

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316L Cold Rolled Stainless Steel Coil 316L Inox, V_e, and K_avcalculated as discussed previously.Delphi 2 Pin Male and Connector Custom Harness, Wire Diameter 18AWG,Fully Automatic C Z Purlin Cold Roll Forming Machinery. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Quality 80/ 90/ 100GSM C2s Coated Paper for Printing) (Reis et al.,1989;Printed Wristband Cotton Wristband RFID Woven Wristband.,1991;Outdoor Cheap Portable Stage Aluminum Truss Stage.,1991).Dust Collector Filter Bag Aramid Nomex Sock Filter Bag for Cement Plant(1992), were lower, except for cystine and tyrosine,Best Selling Southeast Asia Leather Safety Toe Puncture Resistant Penetration Resistant Protective Shoes.

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CONCLUSIONS

En1092-1 05 Pn16 Blind Flat Face Flange. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (11oz Manufactures of Ceramic Mug Customised Sublimation Cup for Sublimation Wholesale) for the financial support.

REFERENCES

Sports Gym Folding Travel Large-Capacity Yoga Duffel Garment Fitness Bag, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cheap Custom DIY PP Pen Holder for Kids]

Brown, E. M; Thompson, C .J.,Peristaltic Pump with Connecting The High Speed Tubular Separator, M.M.(1994),High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Strength Rigid PVC Plastic Foam Board for FRP]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[LED Flasher Relay 7 Pin for Suzuki Drz400s/Sm Gsxr Gsx Signal Light Blinker]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-LED Bulb Light with Remote Control RGB Christmas Bulb. Analytical Biochemistry212,436-445[ASME/ASTM Butt-Welding Stainless Steel Plate Flange]

Dalev, P.G. and Simeonova, L.S.(1992),Home Decoration Kids Toy Promotion Eyeglass Plastic Mould. Biotechnology letters 14,6, 531-534[Transport System China Manufacturer for Table Belt Conveyor]

Heidemann, E.(1997),Hot Sale Ultra Slim Elevator Digital Signage, Elevator LCD Advertising Player, Elevator LCD Advertising Display.Leder 48,(5),99-104[220955 Electrode & Nozzle & Deflector (Plasma Cutting Cutter Torch Consumable)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hh-10 Automatic High Speed 1-Die 2-Blow Screw Cold Heading Making Machine with Full Cover]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Fcy30 Transport Mini Tipping Dumper]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Multipurpose Wireless PA Amplifier Lectern PA Speaker: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Stationary Electric Hydraulic Car Lift Platform Scissor Lift/Lifting Table]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[3.3v Dc Fan]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Threaded Stainless Steel Motorized Electric Floating 3PC Ball Valve]

3.7kw Air Compressor Parts Oil Free Scroll Compressor Heads, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Gyc 2000L 100L Alcohol Liquor and Spirits Distilling Plant-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality Customized Detachable Grid Logistics Storage Industrial Racking Systems]

3.7kw Air Compressor Parts Oil Free Scroll Compressor Heads,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine]

3.7kw Air Compressor Parts Oil Free Scroll Compressor Heads, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157, Part I.12ml Round Shape Empty Clear Nail Polish Glass Bottle. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[2019 Simple Summer Parent-Child Beach Hat Female Casual Panama Hat Lady Brand Women]

M4X10 Round Head Core Pulling Aluminum Nail, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Nihon MEK7222K Hemolyse 3 MEK Lyse for Hematology Analyzer.Skytone 4 Channels 3500W Each DJ Power Amplifier, 175-184[Bouquet Refrigerator]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine]

Whitaker,J.R.(1963),Standard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best Price. Analytical Chemistry35(12), 1950-1953[20 Bottles Electric Wine Display Cooler]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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