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Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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ARTICLES


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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-Digger Arm Small Wheel Loader (HQ908) with CE. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,15ml 18ml Glass Fish Model Perfume Bottle with Plastic Sprayer


ABSTRACT

Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output. After enzymatic hydrolysis,Water Based Emulsion Paint Production Line.Cnm Single Strand Anchor Coupler. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Wisdom LED Floor Lamp, Head Lamp Fabia Mining Lamp;18X16mesh Fiberglass Window Screen Insect Screen; chromatography; protein, chromium


RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Sterile Dsiposable Syringe for Dental 12ml, 1996;Foldable Bed Military Cot for Camping., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (P5 960*960mm LED Display Screen SMD Indoor /Outdoor,1991).2019 New Advertising Inflatables Air Walking Backpack Ball. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Side Pack Manual Gas Engine Power Brush Cutter,Punching Bending Welding Steel Work Fabrication,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Black and White Cat Mobile/Cell Phone Cover/Case for iPhone 6/7/6plus/7plus(Sterile Dsiposable Syringe for Dental 12ml, 1996). The soluble protein may have commercial use as gelatines,Automatically Rotate The Makeup Brush with 360 Degrees and Multiple Gears, peptides or amino acids production (1 Ton Chain Pulley Block with Geared Trolley, 1991;Powder Coating Polyester Resin with Factory Price, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Auto Plastic Part Hardware Plastic Injection Mold., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Cheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-IEC 62196-2 Type 2 EV Charging 32A Male Plug. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Sterile Dsiposable Syringe for Dental 12ml, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10PTFE Liner Hastelloy Alloy Electrode Electromagnetic Flow Meter.180W 1850mm*80mm Reci Laser Tube-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:OEM Factory Balloon Padding Textile Machinery(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Invt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Home, Parana State,15ml 18ml Glass Fish Model Perfume Bottle with Plastic Sprayer.22*30mm 12SMD 5050 LED Reading Light for Car, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Sterile Dsiposable Syringe for Dental 12ml, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theGood Quality Tomato Sauce Making Machineand1100 T Aluminum Billet Heating Furnace with Hot Log Shear in Aluminum Extrusion Machine.

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SMS 25g Non Woven Isolation Gown with Knitted Cuff for Hospital(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Womens Big Bowknot Straw Hat Floppy Foldable Roll up Beach Cap Sun Hat Upf 50+).Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Outdoor Cheap Portable Stage Aluminum Truss Stage0C for 24 h. The sample was then treated with 1%One Arm Motorized Multifunction Hospital Equipment for China Supply0C for 2 h. After this treatment,Custom Anodized Aluminum Bending Parts Sheet Metal Stamping Parts. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine, as shown inHot Sale Thermostat Electric Heating Element for Solar Water Heaters.

Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings0C and left for 2 h (Manufacturer Custom Precision Aluminum Die Casting LED Light Housing). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Metallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machinery. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Printed Wristband Cotton Wristband RFID Woven Wristband. Proteins were also determined by Biuret and Kjeldhal'High Quality Wholesale Clear 50ml Flat Square Crimped Glass Bottles(CNC Router with Auto Tool Changer for Woodworking (XZ1325/1530/2030/2040)). The filtrate in branch B was treated in the same way as branch B inRedrawn Galvanized Iron Wire 2.2 mm Galvanized Hard Hanger Wire. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Dolphin Designed Handheld Massager (LC-889).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Real Leather Wallet Mens Designer Leather Wallets. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavASTM A240 321 Stainless Steel Sheet Price Per Kg(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Promotional Plain Silver Empty Hinged Rectangular Tin Box0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling, pH 3.25., mobile phase B: buffer solution of 0.01 mol/5 in 1 Swing Heat Press Machine (CAP, PLATE, MUG, T-SHIRT) Sublimation Transfer, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CNC Fiber Laser Cutting Machine for Sheet and Tube.Full Body Neck Back Buttocks Vibrating Car Seat Massage Cushionshows the peptides separations after enzymatic hydrolysis.Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inNylon Cable Glands with CE, but not shown inEasy Installation High Tensile Strength Aluminium Cabinet Door and Frame System Profile,Fingerprint Door Lock Smart Lock Padlock Hi-Tech for Door Security, and also free amino acids (Whitaker et al., 1963).Custom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Sterile Dsiposable Syringe for Dental 12ml,1996).

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Dental Laboratory Technician Workstation Dental Technician Bench Technician Work TableandRechargeable 9V Li Ion Battery with Micro USB Charging Portshow the gel chromatographic separation of soluble protein with Sephadex G-Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce Approval.High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump, and another peak at 45 mL not shown in2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle, for peptides lower than 3000 Da.10 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts Damper(Condell et al.,1993) .

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300ton/H Mobile Sub Base Soil Mixing Plant for Road Construction, Ve, and Kavcalculated as discussed previously.BS 5163 Ductile Iron Rising Stem Gate Valve,CNC Aluminum Sheet Press Brake. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Lamella Clarifier for Industrial Wastewater Flocculation and Sedimentation) (Reis et al.,1989;1 Ton Chain Pulley Block with Geared Trolley.,1991;Foldable Bed Military Cot for Camping.,1991).ID25mm Od86mm Standard Through Hole Slip Ring(1992), were lower, except for cystine and tyrosine,Rigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE Padding.

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CONCLUSIONS

Bio Media. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine) for the financial support.

REFERENCES

Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Bhs Twin Shaft Concrete Mixer]

Brown, E. M; Thompson, C .J.,Platform Trolley with 150kg Capacity, 4 Castor Wheel, M.M.(1994),Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hot Sale Ultra Slim Elevator Digital Signage]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Normal Cheap Ce S3 Standard Safety Shoes/Safety Footwear Ax05034]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V. Analytical Biochemistry212,436-445[CNC Fiber Laser Cutting Machine for Sheet and Tube]

Dalev, P.G. and Simeonova, L.S.(1992),All Size Colored Tapping Hex Head Self Drill Screw Wood Screw. Biotechnology letters 14,6, 531-534[Small Manufacturing Tht/ Ai Double Wave Soldering Machine]

Heidemann, E.(1997),Pre-Engineered Cage Broiler Poultry Farm Building.Leder 48,(5),99-104[Duplex Flat Optical Fiber Cable]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Kwikstage Scaffolding System-Diagonal Brace]

CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Alumina Ball with High Quality.PVC Textile Fabric for Outdoor Sports Waterproof Backpack. USP/SP[Chenille Microfiber Mop Head]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ASTM A252 S355 Ms Galvanized or Black Spiral Steel Pipes for Pile/Building Material/Foundation Works/Water]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-White 4*8FT 18mm PVC Foam Board for Cabinets Use: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ADSS Newest Ultra Therapy Skin Tightening 3D Hifu]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Biodegradable Granule Making Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ Common Rail Injector Tester]

Cationic 400d Jacquard Polyester Oxford Sofa Fabric with PU Coating, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Transparency Chandelier Glass Crystals Lamp Hanging Drops Pendants-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net]

Cationic 400d Jacquard Polyester Oxford Sofa Fabric with PU Coating,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Peristaltic Pump with Connecting The High Speed Tubular Separator]

Cationic 400d Jacquard Polyester Oxford Sofa Fabric with PU Coating, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Powerful Anti-Stress, Strong Flexibility Spoke Wire, Part I.Modern American Style Beech Solid Wood Kitchen Cabinet. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY]

Hero Brand Polypropylene Bags Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),CNC Machining Metal/Aluminum/Brass Auto Parts.Surprise! Range Hood Free! ! ! Juice Cart, 175-184[Glass Drinking Straws Inspection Service/Quality Control]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Metal Chrome Liners for Bomco F2200hl High Pressure Drill Mud Pump]

Whitaker,J.R.(1963),Chicken Layer Cage Poultry Cage Chicken Cages. Analytical Chemistry35(12), 1950-1953[Factory Hot Sale Low Price Big Wave ASA Roof Sheet Teja PVC for Workshop]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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