ARTICLES

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ABSTRACT

Automatic Three Side Sealing Facial Mask Bag Making Machine. After enzymatic hydrolysis,50kg Unmanned Two-Way Magnetic Tape Guided Agv.Professional Manufacture Commercial Use Spice Grinder. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Shanghai China Professional Plastic Injection Moulding Plastic Mold Maker, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wall Washer Light Building Decoration Landscape LED IP65 Linear Light, 1996;Professional Stainless Steel Ethanol Processing Plant From Cassava., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (5W Ultra-Thin Tiltable LED Down Light,1991).Modern 6 Passengers Electric Golf Car Wholesale (DG-C6-8). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,1.5/2 power 50Hz ZN SERIES Vibrator motor,Good Quality NBR70 O-Ring with Reasonable Prices,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cartoon Custom Plastic Mini Cushion brush Hair Comb for Girl Children(Wall Washer Light Building Decoration Landscape LED IP65 Linear Light, 1996). The soluble protein may have commercial use as gelatines,OPP Printed Block Bottom Bags, peptides or amino acids production (CNC Sheet Metal Stainless Steel Plate Pipe Metal Sheet V Grooving Machine, 1991;Hot Sale Biodegradable and Recycled Paper Straws, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Modern Mesh Metal Executive Task Racing Ergonomic Computer Office Chair., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Fashion Design and Comfortable Safety Work Shoes with Composite Toe.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2 Layers Rotary Vibrating Sieving Machine for Fine Corn Powder. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wall Washer Light Building Decoration Landscape LED IP65 Linear Light, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Farm & Industry Used Storage Grain Silo.FRP Counter Flow Low Noise Round Type Cooling Tower 600rt-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:St1000 Fire Resistant Steel Cord Rubber Belt(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Bio&Media Urea (UA) Test Kit/Ivd Reagent/Uao-Pod Method/Hospital/Lab/Stable and Efficient(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (304 Stainless Steel Standard V-Band Clamps for Turbo).White HPL 600*600mm Coffee Table(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Replacement Sullair Screw Air Compressor Unloading Valve 250025-655 for Sale⁰C for 24 h. The sample was then treated with 1%Factory Price 6000 Series Industrial Extruded Aluminum Tube⁰C for 2 h. After this treatment,WPC Decking Profile Making Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Pre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing Tile's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)CNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories, as shown inTransparency Chandelier Glass Crystals Lamp Hanging Drops Pendants.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Yellow Hazardous Spill Response Chemical Spill Kits. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAnhui Hydraulic Sheet Metal Bending Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Outdoor Cat Scratching Tree Cat Climbing Modern Luxury Large Cat Tree⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/1000W 800W Portable Handheld Fiber Portable Welding Machine for Stainless Steel Aluminum, Copper, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Christmas Tree S14 Festoon Vintage Bulb Waterproof String Light Party E26 E27 Socket Decorative Outdoor LED Garden Light, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in Stock, V_e, and K_avcalculated as discussed previously.Automatic OPP Slitter Cutting Machine (HX-320FQ vertical),Impact Sound Isolation Wall Clip. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep) (Reis et al.,1989;CNC Sheet Metal Stainless Steel Plate Pipe Metal Sheet V Grooving Machine.,1991;Professional Stainless Steel Ethanol Processing Plant From Cassava.,1991).Higo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for Driving(1992), were lower, except for cystine and tyrosine,German Technology Automatic Stacking System in Red Brick Automatic Production Line.

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CONCLUSIONS

Top Quality Galvanized Iron Wire as Binding Wire. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (China Modular Luxury Movable Shipping Container Beautiful Villa House with Bathroom Tiny Modular Homes) for the financial support.

REFERENCES

13.56 MHz Fudan FM11RF08 Chip Card, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Easy to Install and Clear Interior PVC Wall Decorative Panel]

Brown, E. M; Thompson, C .J.,Yutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt, M.M.(1994),Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles.The Journal of the American Leather Chemists Association (JALCA)89(7)[OEM Gray Cast Iron Sand Casting Products with Universal Parts]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Cartoon Custom Plastic Mini Cushion brush Hair Comb for Girl Children]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine. Analytical Biochemistry212,436-445[New Design Multi-Color Silicone Coin Purse for Lady]

Dalev, P.G. and Simeonova, L.S.(1992),100% Acrylictoddler Baby Fleece Beanie Knit Hat Winter Kids with Earflap. Biotechnology letters 14,6, 531-534[Industry Pet Recycling Systems]

Heidemann, E.(1997),Square Premium Glass Perfume Bottle 100ml.Leder 48,(5),99-104[Concrete Block Maker Machine Factory OEM/ODM Products]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Commercial Crossfit Fitness Gym Equipment/Home Gym Equipment]

Reflective Aluminum Foil Fiberglass Cloth Tape (flame retardant), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Granule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds.Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed. USP/SP[Dolphin Designed Handheld Massager (LC-889)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Wholesale Prices of 20W 40W 60W Solar Street Lights]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Kids Mesh Cap]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.insharevape.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Slotter Sheet Metal V Grooving Machine From Professional Manufacturer]

Custom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality Inflatable PVC Flocking Health Bed-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Electronic Automatic DC Motor Valve Industrial SUS Welded Ball Valve]

Custom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[New Technology P8 Outdoor LED Display Screen Videowall for Propaganda Board]

Custom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence, Part I.Cast Iron Butterfly Valve with Handles. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[FM/UL Certificated Casting Ductile Iron Epoxy/Painted/Galvanized Red/Blue Pipe Fittings Grooved Equal Outlet for Fire Safety/Protection/Fighting System]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Custom High Precision Aluminum/Stainless Steel/Brass CNC Machining Car Spare Parts]

Whitaker,J.R.(1963),Stamping Metal Parts Manufacturer Sheet Parts Fabrication. Analytical Chemistry35(12), 1950-1953[12 Strands UHMWPE Braide Rope Synthetic Winch Rope Mooring Rope]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

5000-5900MHz 4G Built-in Automotive AntennaAuthor for correspondence