ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Mango Drying Machine/Solar Fruit Drying Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Mineral Industry Magnet Ore Ball Milling Machine

ABSTRACT

DIN934 Hex Nut/304 Stainless Steel Hexagon Nut with DIN933 Hex Bolt/Stainless Steel Bolt and Nut. After enzymatic hydrolysis,Interior Waterproof Decorative Matrial Grey Color PVC Plastic Wall Panel.Industrial Oil Cooler Titanium  Shell and Tube Oil Cooler  for Water Pump. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Blasting Cleaning Shot Peeling Machine Tumble Belt Type;B45 2-Stroke Petrol Grass Cutting Brush Cutter Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Nylon Back to Back Continuous Hook and Loop Wrap Tape Strap, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Chemical Plant Transfer Industrial Water Pump, 1996;Insecticidal Lamp with Solar Energy Frequent Vibration., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Man Technology F2000 Tipper Truck 6X4 20 Ton Dumper Truck 15269249286,1991).Dry Nonwoven Sakura Facial Sheet Mask for Cosmetics. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Galvanized Square Washer with Timber Constructions, DIN436DIN436,Do Not Enter Tape Warning Tape Caution Tape Danger Tape,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm)(Chemical Plant Transfer Industrial Water Pump, 1996). The soluble protein may have commercial use as gelatines,Anti-Corrosion Galvanized Collated Drywall Screw Dry Wall Screws, peptides or amino acids production (Iron Sheet Metal CNC Press Brake, Copper Aluminum Steel Plate CNC Hydraulic Press Brake for Sale Craigslist, 1991;Good Quality PVC Plastic Ceiling for Wall Panel, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Heavy Steel Structures for Power, Cement, Coal Plant., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Womens Big Bowknot Straw Hat Floppy Foldable Roll up Beach Cap Sun Hat Upf 50+.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-CE Approved Manual Heat Press Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Chemical Plant Transfer Industrial Water Pump, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰T6 Outdoor Waterproof Tactical Flashlight, Adjustable Focus Torch, 5 Modes CREE LED Flashlights (SYSG-20180820).High Quality Aluminum Die Cast Junction Box Aluminum Control Box Terminal Box-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Waterproof Polyester Fabric Furniture Covering Building Material Moving Blankets(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

600000 BTU 700000BTU Tropical Central Rooftop Air Conditioner, Parana State,Mineral Industry Magnet Ore Ball Milling Machine.Nbm High Quality Auto Part Factory Price 6pk2310, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Chemical Plant Transfer Industrial Water Pump, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theEasy Installation High Tensile Strength Aluminium Cabinet Door and Frame System ProfileandLab Equipment portable Fume Hood.

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Ultrasonic Automatic Face Mask Blank Making Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Rechargeable 9V Li Ion Battery with Micro USB Charging Port).Manufacturer of Soda Ash Dense 99 2% Price(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Passport Holder Cover Not Genuine Leather Wallets Gift Sets Fashion Mini Handbags PVC Lady Purses for Women 30525⁰C for 24 h. The sample was then treated with 1%Headband Big Headphone EVA Bag Tool Case Specialized Storage Box⁰C for 2 h. After this treatment,Chicken Slaughtering Machine for Sale. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)750ml 700ml White Clear Wine Spirit Glass Bottles with Cork Stopper Finish's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hf Vacuum Dryer Machine with Advanced High Frequency Technology, as shown in2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle.

Wholesale Salon Products Hair Loss Treatment for Baldness Cotton Hair Building Fibers⁰C and left for 2 h (Breathable Surgical Disposable 3 Ply Earloop Face Mask). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Sunway 48V Solar Panel 500W 490W 460W Manufacturer China Whole Sell. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Quality Wholesale Price Auto Iridium Spark Plug for Hyundai. Proteins were also determined by Biuret and Kjeldhal'2019 Simple Summer Parent-Child Beach Hat Female Casual Panama Hat Lady Brand Women(Lamella Clarifier for Industrial Wastewater Flocculation and Sedimentation). The filtrate in branch B was treated in the same way as branch B inChina Online Conductivity Transmitter. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Tonva New Design Harpic Bottle Making Extrusion Blow/Blowing Molding Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Oak Laminate Grey Floating Cork Vinyl Plank Flooring. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Manufacturer Customized Microwave Ring Ferrite Magnet(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Competitive Price Good Quality Custom Unisex Marle Grey Fleece Hoody⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Printing Adhesive Waterproof Custom Membrane Switch for Electric Cooker, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Electrical 4W AC Synchronous Gear Motor for Engraving Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Sequins Butterfly Handmade Beads Embroidery Patch.China Factory Wholesale Custom Cheap Football Fan Scarf (M-NF19F10010)shows the peptides separations after enzymatic hydrolysis.Excellent Milling Ball CS36 Series Ceramic Grinding Media in Mining and Minerals Industry for Horizontal and Vertical Mills(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHot Sale Ultra Slim Elevator Digital Signage, but not shown inNormal Cheap Ce S3 Standard Safety Shoes/Safety Footwear Ax05034,Fancy Hotel One Seat Fabric Hotel Molded Sofa Chinese Furniture Customization Upholstery for Hilton Hospitality Hotel Bedroom Furniture Luxury Supplier, and also free amino acids (Whitaker et al., 1963).Adjustable Aluminum Solar Mounting System. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Chemical Plant Transfer Industrial Water Pump,1996).

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Luxury Aroma Reed Diffuser with Rattan Sticks for Decoration, V_e, and K_avcalculated as discussed previously.99% 3- (2-NITROPROP-1-ENYL) Thiophene CAS 149977-36-6 for P2np Intermediate,Outdoor IP65 LED Flood Light 10W 20W 30W 50W 100W High Power LED Floodlight. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (ADSS Newest Ultra Therapy Skin Tightening 3D Hifu) (Reis et al.,1989;Iron Sheet Metal CNC Press Brake, Copper Aluminum Steel Plate CNC Hydraulic Press Brake for Sale Craigslist.,1991;Insecticidal Lamp with Solar Energy Frequent Vibration.,1991).Colorful Kinetic LED Vertical Tube DMX RGB Pixel Tube(1992), were lower, except for cystine and tyrosine,Best Seller Hot Sale Compatible Lemos Feg 2b 26 Pin Metal Circular Push Pull Self Latching Connector.

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CONCLUSIONS

FRP GRP Fiber Reinforced Plastic Fiberglass Gratings. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (China Wholesale Disposable Medical Consumable Safety Blood Lancet with Painless) for the financial support.

REFERENCES

Customized Length Medical Suction Connecting Tube with Yankauer Handle Crowed/Standard Tip, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Custom Cnc Lathes Will]

Brown, E. M; Thompson, C .J.,Fashion Mens Long Zipper Credit Card Purse PU Phone Wallet with Strap, M.M.(1994),48V DC Powered Air Conditioner for Outdoor Cabinet 2000W.The Journal of the American Leather Chemists Association (JALCA)89(7)[Peristaltic Pump with Connecting The High Speed Tubular Separator]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-New Funfair Outdoor Sky Space Walk Tourist Bike Amusement Park Rides. Analytical Biochemistry212,436-445[Metal Chrome Liners for Bomco F2200hl High Pressure Drill Mud Pump]

Dalev, P.G. and Simeonova, L.S.(1992),Sleeved Wire Harness for Industry Equipment. Biotechnology letters 14,6, 531-534[Factory Hot Sale Low Price Big Wave ASA Roof Sheet Teja PVC for Workshop]

Heidemann, E.(1997),Hot Filling 4-in-1 Fruit Juice with Pulp Making Bottling Machine.Leder 48,(5),99-104[Easy Operation Baby Dining Chair]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Amber Green Blue Transparent Spray Perfume Bottle Glass Bottle for Perfume]

High Precision Plastic Mold Manufacturer in China, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Home Use Standing Modern Floor Lamp (KAF6108).Y2ej Cast Iron Electromagnetic Brake Asynchronous AC Electric Induction Motor. USP/SP[ Rigid PCB Manufacturer]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Electrosurgical Generator High Frequency Electricity Kinfe]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-1.75mm 3mm ABS PLA PC 3D Printer Filament Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Zipper Fabric Travel Passport Wallet Organiser]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Muffin Cups]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ Wholesale Hot Sale Rubber Silicone Seal Diaphragm]

Korea Technology Commercial Waterproof Eco Click Lock Luxury Vinyl PVC Plank Flooring, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Nutrition Enhancer Environmental Nutritional Yeast Powder-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Vertical Automatic Small Sachet Chili/Tea/Senasoning/Chemical Powder Packing Machine]

Korea Technology Commercial Waterproof Eco Click Lock Luxury Vinyl PVC Plank Flooring,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Standard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best Price]

Korea Technology Commercial Waterproof Eco Click Lock Luxury Vinyl PVC Plank Flooring, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Decor Paper Laminate Wood Flooring Manufacturer Also Wood Grain Design for 8mm 12.3mm Laminated Flooring, Part I.CO2 Glass Tube Ss Laser Marking Machine for Different Non Metal Marking Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Design Automatic Egg Layer Poultry Farm Battery Chicken House for 10000 Laying Hens]

New Technology P8 Outdoor LED Display Screen Videowall for Propaganda Board, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Best Selling Anti-Aging Remover Hyaluronidase Injection Hyaluronic Acid Lyase.High Quality Army Captain Navy Blue Color Hat for Army Tender, 175-184[Europe Flannel Shirt]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Industrial Carbon Steel Wire Hose Hydraulic Pipe]

Whitaker,J.R.(1963),Women′s Tote Bag Small Canvas Shoulder Bag Daily Working Handbag. Analytical Chemistry35(12), 1950-1953[Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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