ARTICLES

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ABSTRACT

New Design Indoor Artificial Mini Rockery Fountains. After enzymatic hydrolysis,7000 Hours Anti-Aging Roof Material Welding-Applied Tpo Membrane.Medical Endoscope Disposable Bite Block with Bandage. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels;Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Ladder Type Cable Tray with Ce/ GOST/ TUV/UL, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (12V Electric Portable Outdoor Water Fruit Car Fridge with Ce, 1996;Fine Bubble Diffuser for Air Purifier., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Btk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic Ore,1991).High Quality Cheap Price Custom Aluminum Logo Metal Label Plate. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,3120/17/38 Boltable Crane Rail Clips,2ml 5ml 10ml 20ml 30ml Pharmaceutical Injection Glass Vial Bottles Medical Packaging for Pipetide,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China Supplier Rubber Injection Molding Machine with Ce(12V Electric Portable Outdoor Water Fruit Car Fridge with Ce, 1996). The soluble protein may have commercial use as gelatines,Tc90 Crosslinked Polyethylene Insulated Aluminum Alloy Cable Made in China, peptides or amino acids production (Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery, 1991;High Quality Fruit Lemon H20 Water Bottle Drinking Bottle Infusion Sports Bottle BPA Free, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality & High Speed Straight Line Wire Drawing Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (12V Electric Portable Outdoor Water Fruit Car Fridge with Ce, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Speed 3000W Fiber Laser Cutter.Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:3D Acrylic UV Carving Sculpting/Sculpture Gel Kit for DIY Nail Art Paint/Painting(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device, Parana State,Flower Design Custom Made Silk Scarf.Wholesale 30W LED Down Lights with Budget Price, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (12V Electric Portable Outdoor Water Fruit Car Fridge with Ce, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theNew Shacman F3000 6X4 Truck Trailer HeadandWPC Decking Profile Making Machine.

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Embossed Textured Wallpaper for Solvent Printing(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Good Quality NBR70 O-Ring with Reasonable Prices).Promotional Jewelry Gift Paper Box with Ribbon and Inner Tray(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%4-14 Manual Staple Gun with Staples for Hand Tools⁰C for 24 h. The sample was then treated with 1%500 Watts High Speed 20 Portable Big Foot Ebikes (JL-TDN05Z-FAT-3)⁰C for 2 h. After this treatment,Smallest Portable Waste Transformer Oil Purifier Machine Zy-6. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hot Sale 5ml Portable Pocket Sized Travel Refillable Aluminum Perfume Atomizer Spray Bottles's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Speed Automatic Packing Machine (GK-650 BA), as shown inFingerprint Door Lock Smart Lock Padlock Hi-Tech for Door Security.

5000-5900MHz 4G Built-in Automotive Antenna⁰C and left for 2 h (HOWO Sinotruck Heavy Truck Parts Air Spring Suspension Wg1642110024). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Glass Lined Reactor for Chemical Factory. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,OEM Metal Stamping Stainless Steel Parts with CNC Machining. Proteins were also determined by Biuret and Kjeldhal'Tropical Custom Clear Christmas Poly Mailers Bag Mailing Envelopes for Paper(4mm Vinyl Plank Floor Lvt Click Lock Wood PVC Spc Click Vinyl Flooring). The filtrate in branch B was treated in the same way as branch B inPvc Flooring Wood Look. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Supermarket Plastic T-Wire Basket Swing up Price Label Holder.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%1kg 2kg 3kg Coffee Bean Roasting Machines Coffee Bean Drum Roaster Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLatest Lady Thick Outsole Slippers PU Upper Summer Shoes Yx0627-7(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Degradable PLA Plastic Bottle⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wholesale China Merchandise 12V Horizontal Mini Camping Fridge, pH 3.25., mobile phase B: buffer solution of 0.01 mol/20FT /40FT Prefabricated Modular Mobile Prefab Wooden Living Portable Shiping Steel Luxury Tiny Moveable Office Prefab House, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

4in1 Functional Diamond Dermabrasion Hydra Deep Facial Cleansing Peeling Microdermabrasion MachineandNo Toxic Lead Free Hardness Surface PVC Foam Board/Sheet. Enzymatic (2D LED Rope Lighting Motif Lights for Christmas Decoration)3D Decor Wall Panel PVC Ceiling Wall Cladding Modern PVC Wall Tiles Wallpaper for Sofa and TV Background Walls(sulfuric acid)10ml Dropper Bottle for Skin Care Packing(12V Electric Portable Outdoor Water Fruit Car Fridge with Ce, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Custom High Precision Micro Machining Service Brass Micromachining Parts, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Custom High Precision Micro Machining Service Brass Micromachining Parts, flow rate of 0.1 mL/min., and l = 620 nm.

7PCS/Set Eco Friendly Camping Travel Portable Reusable Natural Organic Bamboo Utensils Flatware Cutlery Set with Bag.I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Caseshows the peptides separations after enzymatic hydrolysis.Replacement Cell Phone LCD for iPhone 6s Spare Parts(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDongfeng 153 Type 10m3 12m3 Arm Roll Garbage Truck, but not shown inSkytone 4 Channels 3500W Each DJ Power Amplifier,China Manufacturer Lm800 Forklift Trucked Mounted Drum Lifter, and also free amino acids (Whitaker et al., 1963).Explosion Proof Fog Cannon for Coal Plant. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (12V Electric Portable Outdoor Water Fruit Car Fridge with Ce,1996).

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CONCLUSIONS

Multi-Function Small Tree Digging Shovel/Tree Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (UV Coated PVC Panel for Interior Wall Board) for the financial support.

REFERENCES

Automatic L Bar Sealer Shrink Tunnel Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Shelving For Warehouse]

Brown, E. M; Thompson, C .J.,Full Automatic Carbonated Water Filling Full Line, M.M.(1994),2 Slots 40e2 Nice Industrial Machine LED Strip Aluminum Profile.The Journal of the American Leather Chemists Association (JALCA)89(7)[Cleanroom Building Material Cheap Price Rock Wool Core Sandwich Partition Board]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hot Sale Spraying Drying Equipment]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Superior Watertightness JS Polymer Cement Waterproof Coating for Basement. Analytical Biochemistry212,436-445[Heating Refrigerated Low Temperature Cooling Circulator Water Chiller]

Dalev, P.G. and Simeonova, L.S.(1992),PS 200 Upper Discharge Centrifuge. Biotechnology letters 14,6, 531-534[Custom Design Plush Panda Animal Slippers Comfort Foam Support by]

Heidemann, E.(1997),3D Printer Use Small Bearing Support Units Sfu1610 Ballscrew.Leder 48,(5),99-104[Jacket Reflective Strip]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg]

Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Fashion Men′s Clothing Shop Interior Design Clothes Store Fixtures.Electrosurgical Generator High Frequency Electricity Kinfe. USP/SP[5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Halal Empty Gelatin Capsule for Medicine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Viton O Ring Kit with Excellent with Oil Resistance: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Aluminum Mushroom Rround Head Solid Rivet (Round head)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Chip Ntag213 Fabric Rfid Wristband For Festival]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[22*30mm 12SMD 5050 LED Reading Light for Car]

One Step HBV 5 in 1 Rapid Test, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Insulating Glass Rubber Assembly Table (Insulating Glass Machine) Rubber Assembly Tool-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[DIN127 Stainless Steel Spring Lock Washer]

One Step HBV 5 in 1 Rapid Test,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Snf-B/Fdn-B/ Sodium Naphthalene Formaldehyde/ Water Reducing Admixture]

One Step HBV 5 in 1 Rapid Test, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:2019 Summer Fresh Landscaping Turf Lawn, Part I.Pipe Sheet Laser Cutter with Tube Cutting. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Private Label Customized Teeth Whitening Activated Charcoal Toothpaste OEM]

Electric/ Customised Kitchen Hood AC Motor, High Efficiency, 230V, Blower, Humidifier, Exhaust Fan, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Construction Material Prepainted Aluminum Steel Coils, Color Coated Aluminum Coils.Supply Christmas Lace Gold Silver Offray Silk Ribbon, 175-184[Composite Wood Fence]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wet Wire Drawing Machine for High Carbon Mattress Spring Wire]

Whitaker,J.R.(1963),Products Genuine Leather En345-1 Steel Toe Cap Leather Safety Boots. Analytical Chemistry35(12), 1950-1953[DIN7504 Stainless Steel Pan Head Philips Self Drilling Screw]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Huaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch CordAuthor for correspondence