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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-AAC Block Production Line / AAC Equipment Manufacturing/ Machinery. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,CB-III (2011) Medical Equipment Tractional Chinese Medicine Fumigation Machine

ABSTRACT

Q32 Series Tumble Belt/Crawler Shot Blasting Machine. After enzymatic hydrolysis,Galvanized Bar Grating.High Quality Custom Self Adhesive Leather Patch for Jackets. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Sinicline Custom Printed Decorative Self Sealing Plastic Poly Bubble Mailers Mailing Bags Courier Bag for Postage Shop Online for Sale;380mm Working Area Concrete Diamond Floor Edge Grinder; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Angle Iron Steel Purlin Q235/345 Equal Angle Steel / Hot Dipped Galvanized Beam, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (New Mobile Smartphone for Xiaomi 9 PRO, 5g Android Phone, 8GB 256GB Unlocked Mobile Phone Wholesale for Huawei Series Mate, 1996;Discussion and Interpretation System., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input,1991).Frozen Soybean in Pod. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Custom Sheet Metal Stamping Bending Product Manufacturing Service,Waterproof EVA Rubber Roofing Material for Shingle Building,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,OEM Gray Cast Iron Sand Casting Products with Universal Parts(New Mobile Smartphone for Xiaomi 9 PRO, 5g Android Phone, 8GB 256GB Unlocked Mobile Phone Wholesale for Huawei Series Mate, 1996). The soluble protein may have commercial use as gelatines,U. S. Type Drop Forged Wire Rope Clip, peptides or amino acids production (Humizone Seaweed Customized Organic Liquid Fertilizer, 1991;Zipper Fabric Travel Passport Wallet Organiser, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot Sale Instax Mini Camera Korea Style Photo Album., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Aluminum Low Profile Adjustable Bracket.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Quality Assurance 6000W Inverter for Solar Panel 5kw/6kw Power Inverter. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (New Mobile Smartphone for Xiaomi 9 PRO, 5g Android Phone, 8GB 256GB Unlocked Mobile Phone Wholesale for Huawei Series Mate, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Acrylic Beer Display Racks with LED.Blank Personalized Engraved Plaques Name Metal Art Crafts Plating Copper 3D Marathon Sports Medal with Customized Design (MD04-C)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Baby Feeding Chair Plastic Chair for Feeding Hebei China(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Color Cardstock Color Paper Manufacturer(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (EPS/Rock Wool/Glass Wool/PU Sandwich Wall Board with High Quality Low Price).Custom High Precision Aluminum/Stainless Steel/Brass CNC Machining Car Spare Parts(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Home and Office PVC Vinyl Flooring Conductive Plank Flooring⁰C for 24 h. The sample was then treated with 1%Precision Engineering Parts Bearing Support Unit for Ball Screw⁰C for 2 h. After this treatment,LED Table Lamp LED Desk Lamps LED Table Light 5W DC5V 1200mAh Modern Fashion LED Baby Study Light Lighting Eye Protection. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)165cm Realistic Full Solid TPE Sex Doll Big Breast Love Doll for Man's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Silicone Cake Mold Sinle Cupcake DIY, as shown in Metal Window Screen.

Plastic Pipe Clamp for Refrigeration Pipe System Pipe Fitting⁰C and left for 2 h (High Quality Casting Cover Aluminum Die Casting Products). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.10g Food/Pharmaceutical Desiccant Silica Gel for Moisture Absorbing. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Cross Stripe Sleeveless Yoga Wear. Proteins were also determined by Biuret and Kjeldhal'Construction & Decoration ERW Square Steel Tube Manufacturer(PVC/PP/PE Twin Screw Plastic Granules Extruding Granulator Machine). The filtrate in branch B was treated in the same way as branch B inCustom Zinc Aluminum Alloy Paint Plating Die Casting Parts Housing for Electronic Smart Cabinet Lock Safety Digital Cabinet Locker Password Lock. The final soluble protein shown were used for peptides separations using gel permeation chromatography,DVD Audio RCA Wiring Harness Cable for DVD Pioneer Avh.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Black Steel ASTM A192 Seamless Pipe Sch120. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Excavator Radiator /Oil Radiator /Water Radiator Manufacture(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.BOPP CPP Laminate Food Packaging Metalized Pet Cup Sealing Film⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Metal Trapezoidal Roof Sheet Roll Forming Machine for Roof Panel, pH 3.25., mobile phase B: buffer solution of 0.01 mol/CNC Machining Checking Auto Car Checking Fixture, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Food Grade Stainless Steel Milk Dairy Equipment for FactoryandStainless Steel Thread Ends Sanitary Butterfly Valve with Pull Handle. Enzymatic (T Series Bevel Helical Gearboxes for Sale)Military Police Army Outdoor Protective Safety Bulletproof Camouflage Protective Vest(sulfuric acid)Colorful PVC Foam Board with High Density and Customizable Size(New Mobile Smartphone for Xiaomi 9 PRO, 5g Android Phone, 8GB 256GB Unlocked Mobile Phone Wholesale for Huawei Series Mate, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Air Vent Diffuser Adjustbable Air Well and Jet Diffuser, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Air Vent Diffuser Adjustbable Air Well and Jet Diffuser, flow rate of 0.1 mL/min., and l = 620 nm.

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Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B), V_e, and K_avcalculated as discussed previously.Lm Series Miniature Lm8uu Liner Ball Bush Bushing Ball Bearing,Hot Sale 8 Inch Wheelchair Rubber Wheel Solid PU Small Tire. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (9~12 Inch Mechanical Backing Plate) (Reis et al.,1989;Humizone Seaweed Customized Organic Liquid Fertilizer.,1991;Discussion and Interpretation System.,1991).Hanging Necklace Fan USB Rechargeable Battery Operated Fan(1992), were lower, except for cystine and tyrosine,Aluminum Mushroom Rround Head Solid Rivet (Round head).

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CONCLUSIONS

Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Round Type Twin Screw Extruder Barrel Manufacturer) for the financial support.

REFERENCES

Warm Women Suede Leisure Snow Boots for Winter (FF-1), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Instrument For Spe Cartridges& Plates]

Brown, E. M; Thompson, C .J.,Good Quality Xanthan Gum Application of Food with Best Price, M.M.(1994),Wet Wire Drawing Machine for High Carbon Mattress Spring Wire.The Journal of the American Leather Chemists Association (JALCA)89(7)[Chicken Layer Cage Poultry Cage Chicken Cages]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-120W Ultra Light Solar Blanket Panel for Camping, Caravan, Motorhome. Analytical Biochemistry212,436-445[ASA-UPVC Anti-Corrosive Composite Roof Tile]

Dalev, P.G. and Simeonova, L.S.(1992),Effluent Treatment Ferrous Sulphate Heptahydrate 98%. Biotechnology letters 14,6, 531-534[Sleeved Wire Harness for Industry Equipment]

Heidemann, E.(1997),Rechargeable 9V Li Ion Battery with Micro USB Charging Port.Leder 48,(5),99-104[Anchors For Concrete Suppliers for Sale]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Promotional Reusable Foldable PP Non Woven Shopping Bag]

Digital Lock Electronic Door Lock Password Keypad Number for Cabinet Door, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Transport System China Manufacturer for Table Belt Conveyor.Antique Gold/Copper Coin/Bright Gold/Copper London Ride Medal. USP/SP[PVC Cross Joint Pipe Fittings Equal Pipe Cross]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Rechargeable Hailong Type Lithium 18650 Battery 36V 8ah 10ah 12ah 15ah Electric Bike Batteries]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Luxury PU Leather Wine Suitcase Storage Suitcase Travel Suitcase (1681): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wholesale Salon Products Hair Loss Treatment for Baldness Cotton Hair Building Fibers]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Black and Yellow Durable Safety Traffic Pet Reflective Tape]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[8p8c Mini RJ45 Female Jack Module Connector RJ45 Connector]

High Quality Mini LED Flood Light SMD Flood Light 50W, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Ferrite Core for Power Transformer (Ee16e)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Modern American Style Beech Solid Wood Kitchen Cabinet]

High Quality Mini LED Flood Light SMD Flood Light 50W,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[8mm Heavy Duty Nails U Shaped Galvanized Staples]

High Quality Mini LED Flood Light SMD Flood Light 50W, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Aluminium Unitized Curtain Wall for Office Building, Gymnasium and Hotel, Part I.China Manufacturer Wallpaper House Decoration Wall Paper. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[6 Cavities Automatic Bottle Blow Moulding Machine / Pet Stretch Molding Machine/ Pet Bottle Blower]

Flexible Magnetic Rubber Sheet Soft Magnetic Tape, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Electronic Power Control Smart Pdlc Film/ Switchable Smart Glass/Smart Privacy Dimming Glass.Electric Wine Bottle Opener K020, 175-184[Industrial Hose Reel]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Supermarket Plastic T-Wire Basket Swing up Price Label Holder]

Whitaker,J.R.(1963),Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen. Analytical Chemistry35(12), 1950-1953[Small Frame Hydraulic Press Machine for Bending Price]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Familu Use Mini Small Pig Feed Pellet MillAuthor for correspondence