ARTICLES

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ABSTRACT

Sanjing PVC Lining Layflat Fire Hose. After enzymatic hydrolysis,Qt10-15 Automatic Concrete Cement Hollow Solid Paver Curbstone Brick Block Making Machine Price in Madagascar.China High Quality and Cheapest Price 15W 18W 20W 6′′ LED Down Light. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Paraffin Wax Fully and Semi Refined 56 58 60 62 From Factory Bottom Price, 1996;Pet PP Rope and Broom Wire Drawing Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cup,1991).PTFE Seat Wafer Butterfly Valve Pn16. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Waterjet Intensifier High Pressure Cylinder Waterjet Cutting Machine Spare Part,China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China(Paraffin Wax Fully and Semi Refined 56 58 60 62 From Factory Bottom Price, 1996). The soluble protein may have commercial use as gelatines,Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01), peptides or amino acids production (Pipe Conveyor Belt, Ep Conveyor Belt/ Nn Conveyor Belt, 1991;Plain Makeup Bag Canvas Cosmetic Bag, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;14inch -16.5inch Student / Beginner Maple Laminated Viola with Free Viola Case, Viola Bow., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Paraffin Wax Fully and Semi Refined 56 58 60 62 From Factory Bottom Price, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry.Factory Wholesale Qualited Polyester Padding Men Jacket-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:5.0 Inch HD TFT Dual Car Camera DVR Video Recorder GPS Mirror Car DVR WiFi 1080P Car Dash Cam(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Metal Steel Frame Membrane Structure Car Parking Shed Cover Design⁰C and left for 2 h (Vertical Type Low Pressure Injection Molding Machine with After-Sales Services (JX-900H)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,300X800mm 3D Ink-Jet Ceramic Interior Bathroom Wall Tile. Proteins were also determined by Biuret and Kjeldhal'Integrated LED Solar Street Sensor Light with Sensor Control for Highway 10W/20W/30W/40W/50W/60W(Sand Castings by Gray Iron Material). The filtrate in branch B was treated in the same way as branch B inSinker Needle S-24-11-3. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Polyester Dust Sport Gym Fabric Shoes Drawstring Bag.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Price(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Chicken Farming Materials Poultry Battery Cages Layer Battery Cage.Top10 1080P H. 264 P2p CCTV WiFi IP Camerashows the peptides separations after enzymatic hydrolysis.1000kVA 6kv 6.3kv Step Down Dry Type Power Electric Transformer(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in CNC Lathe Machine Parts, but not shown inPlastic Injection Molding for Auto Parts,I-Tech 12000HD 2u Space Digital Professional Power Amplifier, and also free amino acids (Whitaker et al., 1963).Rigid Flex PCB China Multilayer Rigid-Flex PCB, Rigid PCB Manufacturer. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Paraffin Wax Fully and Semi Refined 56 58 60 62 From Factory Bottom Price,1996).

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CONCLUSIONS

Stainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Shima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Condition) for the financial support.

REFERENCES

Xjm Series Rubber Extruding Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Quality Steel Plank]

Brown, E. M; Thompson, C .J.,Stop Dog From Jumping or Climbing Your Chain Link Fence., M.M.(1994),SGS Direct Thermal Label for Supermarket.The Journal of the American Leather Chemists Association (JALCA)89(7)[China High Quality and Cheapest Price 15W 18W 20W 6′′ LED Down Light]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Fancy Hotel One Seat Fabric Hotel Molded Sofa Chinese Furniture Customization Upholstery for Hilton Hospitality Hotel Bedroom Furniture Luxury Supplier]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Water Filter Polyester Non-Woven Fabric. Analytical Biochemistry212,436-445[OEM Factory Balloon Padding Textile Machinery]

Dalev, P.G. and Simeonova, L.S.(1992),Industry Pet Recycling Systems, Industry Pet Recycling Line. Biotechnology letters 14,6, 531-534[High Quality Inflatable PVC Flocking Health Bed]

Heidemann, E.(1997),Sublimation Polyester Sports Tshirt for Adult.Leder 48,(5),99-104[Stackable Plastic Hotel Furniture Church Restaurant Dining Banquet Emas Chair]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[White 4*8FT 18mm PVC Foam Board for Cabinets Use]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[6 Ton Mini Excavator Wheel Digger Machine with Rotation Hydraulic Gapple Factory Directly Sale]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fire Extinguisher UV Curing Machine Cylinder Drying Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[CNC Machining Checking Auto Car Checking Fixture]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[External Magnesium Oxide MGO Sulfate Wall Fireproof Board]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Ms Gi Pipe Dn15~Dn400 Gi Pipe List]

Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Energy-Saving Biogas Double Stove for Sale-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose]

Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[One Step HBV 5 in 1 Rapid Test]

Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Valve Bag Dry Mortar Bagging Machine, Part I.High Quality 500m 100lb Super Strong Braided Fishing Line. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Forged Sanitary Stainless Steel Pipe Fitting Hose Nipple]

20mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in China, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance Light.Cheap Alloy Snap Hook Clasps, Lanyard D Ring Gold Metal Swivel Snap Hook Dog Strap, Bag Metal Dog Snap Hook Swivel Bulk for Bags, 175-184[(5X4X4.0mm-4BB) Stainless Steel Ball Bearing Wooden Door Hinge]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Higo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for Driving]

Whitaker,J.R.(1963),PCD Concrete Grinding Cup Wheel Diamond Tools for Urethane Epoxy and Paint. Analytical Chemistry35(12), 1950-1953[3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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