ARTICLES

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ABSTRACT

Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse. After enzymatic hydrolysis,Cow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll.Heat Shrink Automatic Flow Wrapping Packing Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam;Ductile Iron Standard Brackets Pipe Hanger; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Luxury Vintage Throw Pillows Cover for Couch, Damask Pillows Cover, Decorative Pillow Covers, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper, 1996;5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Sheet Metal Bending Roll Forming Machine,1991).Different Size Glass Test Tubes, High Quality Laboratory Test Tubes. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Professional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine,Frame Type Rubber Hydraulic Press Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Kitchen Metal Dish Storage Rack (JP-SC984C)(ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper, 1996). The soluble protein may have commercial use as gelatines,0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming, peptides or amino acids production (AVR Se350 Automatic Voltage Regulator for Marathon Alternator, 1991;Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Excavator Heavy Duty Bolt on Right Hand Sidecutter 232-2147., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Strength Rigid PVC Plastic Foam Board for FRP.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Round, Hexagonal Hole Stainless Steel Perforated Metal Mesh for Building. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall.Threaded Stainless Steel Motorized Electric Floating 3PC Ball Valve-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Replacement Cell Phone LCD for iPhone 6s Spare Parts).High Quality Ceramic Chrome Hybrid Bearing Oil(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Medical Product Nonwoven Disposable Surgical Gown PP Isolation Gown⁰C for 24 h. The sample was then treated with 1%CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder⁰C for 2 h. After this treatment,Digger Arm Small Wheel Loader (HQ908) with CE. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)15ml 18ml Glass Fish Model Perfume Bottle with Plastic Sprayer's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output, as shown in2018 Luxury Design China Supplier Waterproof Pet Cat Dog Bed.

Water Based Emulsion Paint Production Line⁰C and left for 2 h (Soundproofing Foam Backed Vinyl 3D PE Foam Wallpaper Brick Factory). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Cnm Single Strand Anchor Coupler. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Wisdom LED Floor Lamp, Head Lamp Fabia Mining Lamp. Proteins were also determined by Biuret and Kjeldhal'18X16mesh Fiberglass Window Screen Insect Screen(Diameter 2.68m Paragraphed Autoclave for AAC Bricks). The filtrate in branch B was treated in the same way as branch B inBSCI China Supply Waterproof Multi Function Foldable Travel Garment Bags. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Sterile Dsiposable Syringe for Dental 12ml. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFoldable Bed Military Cot for Camping(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.2019 New Advertising Inflatables Air Walking Backpack Ball⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Side Pack Manual Gas Engine Power Brush Cutter, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Punching Bending Welding Steel Work Fabrication, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Cheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machine.Ferrite Core for Power Transformer (Ee16e)shows the peptides separations after enzymatic hydrolysis.IEC 62196-2 Type 2 EV Charging 32A Male Plug(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPS 200 Upper Discharge Centrifuge, but not shown inGlass Manufacturer/Float Glass/ Tempered Glass (Flat or Curved) for Building / Furniture,PTFE Liner Hastelloy Alloy Electrode Electromagnetic Flow Meter, and also free amino acids (Whitaker et al., 1963).180W 1850mm*80mm Reci Laser Tube. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper,1996).

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CONCLUSIONS

Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck) for the financial support.

REFERENCES

Outdoor Cheap Portable Stage Aluminum Truss Stage, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Plastic AWG12-24 Waterproof Electrical 2-8 Pin Wire Plug Connectors]

Brown, E. M; Thompson, C .J.,One Arm Motorized Multifunction Hospital Equipment for China Supply, M.M.(1994),Custom Anodized Aluminum Bending Parts Sheet Metal Stamping Parts.The Journal of the American Leather Chemists Association (JALCA)89(7)[ for Patio Garden Backyard (Warm white)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Zipper Pouch Pistachio Nuts Premade Packing Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display. Analytical Biochemistry212,436-445[Personality Cat Eye Sunglasses]

Dalev, P.G. and Simeonova, L.S.(1992),Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine. Biotechnology letters 14,6, 531-534[Platform Trolley with 150kg Capacity]

Heidemann, E.(1997),Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings.Leder 48,(5),99-104[Furnitture Sofa and Mattress for Pocket Spring /Pocket Spring Unit]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Custom Self Adhesive Leather Patch for Jackets]

Ms-184125-2 1.3mh Arnold Core Common Mode Choke Coils Toroidal Inductor, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Metallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machinery.Printed Wristband Cotton Wristband RFID Woven Wristband. USP/SP[Outsole Trimming Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Airtight Candy Bin with Scoop]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Wholesale Clear 50ml Flat Square Crimped Glass Bottles: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Auto Spare Parts Ignition Coil Fp85-18-100c]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Quality Clear Candy Dried Food Storage Jar Food Container]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine]

Dolphin Designed Handheld Massager (LC-889), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Real Leather Wallet Mens Designer Leather Wallets-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Side Pack Manual Gas Engine Power Brush Cutter]

Dolphin Designed Handheld Massager (LC-889),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Stationery PP Clipboard With 7 Inch Scale Ruler]

Dolphin Designed Handheld Massager (LC-889), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:ASTM A240 321 Stainless Steel Sheet Price Per Kg, Part I.V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Farm Machine Parts Spring Harrow Tines/ Fork Tedder Tines]

Promotional Plain Silver Empty Hinged Rectangular Tin Box, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling.5 in 1 Swing Heat Press Machine (CAP, PLATE, MUG, T-SHIRT) Sublimation Transfer, 175-184[12mm Back Painted Toughened Table Top Glass]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Day and Night Zebra Blind Fabric Z1602]

Whitaker,J.R.(1963),Butyl Glue Self-Adhesive Tape with Radian, Caulk Strip Trim Tape (38mm). Analytical Chemistry35(12), 1950-1953[Customized High Quality Aluminum Alloy Extrusion Enclosure Extruded Case for Power Inverter]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B)Author for correspondence