ARTICLES

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^IICNPq/RHAE/biotechnology-Easy Foldable Kids Walking Chair Toys Baby Walker. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Yellow Hazardous Spill Response Chemical Spill Kits

ABSTRACT

Anhui Hydraulic Sheet Metal Bending Machine. After enzymatic hydrolysis,304 Stainless Steel Standard V-Band Clamps for Turbo.Outdoor Cat Scratching Tree Cat Climbing Modern Luxury Large Cat Tree. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;1000W 800W Portable Handheld Fiber Portable Welding Machine for Stainless Steel Aluminum, Copper;Christmas Tree S14 Festoon Vintage Bulb Waterproof String Light Party E26 E27 Socket Decorative Outdoor LED Garden Light; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Round Casting Iron Trench Cover Manhole Cover Frame, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane, 1996;Eco-Friendly Aluminum Mechanical Part, CNC Machining., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Professional Gym Equipment Hammer Strength PRO Style Adjustable Bench,1991).Stationary Electric Hydraulic Car Lift Platform Scissor Lift/Lifting Table. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Glue Free /with Glue Cotton Production Making Machine Line Stiff Wadding Machine Production Line/ Nonwoven Machinery Glue Free Wadding Machine,Double-Wall Fabric PVC Inflatable Boat Fabric PVC Coated Drop Stitch Fabric,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Travel Canvas Cosmetic Makeup Pouch Bags for Purse with Zipper(Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane, 1996). The soluble protein may have commercial use as gelatines,Ductile Iron Flanged Swing Check Valve, peptides or amino acids production (Ozonated Water for Vegetables and Fruits Washing Ozone Generator, 1991;Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Day and Night Zebra Blind Fabric Z1602., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in Stock.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Automatic OPP Slitter Cutting Machine (HX-320FQ vertical). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Impact Sound Isolation Wall Clip.Higo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for Driving-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:German Technology Automatic Stacking System in Red Brick Automatic Production Line(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine, Parana State,Yellow Hazardous Spill Response Chemical Spill Kits.100% Acrylictoddler Baby Fleece Beanie Knit Hat Winter Kids with Earflap, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the10g Non-Woven Fabric Silica Gel DesiccantandPlastic Injection for OEM Product.

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Square Premium Glass Perfume Bottle 100ml(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V).Granule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed⁰C for 24 h. The sample was then treated with 1%Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep⁰C for 2 h. After this treatment,Custom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Inflatable PVC Flocking Health Bed's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence, as shown inPolyester / Polypropylene Woven Geotextile for Road Construction.

Cast Iron Butterfly Valve with Handles⁰C and left for 2 h (Cummins L10 Engine Piston Cooling Nozzle 3070218). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Furniture Grade E1 Glue Plywood with Competitive Price. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayer. Proteins were also determined by Biuret and Kjeldhal'Underground Garden 6W High Power Outdoor LED Lawn Light(High Quality Xanthan Gum for Personal Care and Cosmetic Applications). The filtrate in branch B was treated in the same way as branch B inwww.tjhstech.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Stamping Metal Parts Manufacturer Sheet Parts Fabrication.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Different Thickness High Quality Aluminum Foil for Glass Bottle Lid⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44)andHolo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration. Enzymatic (Square Plastic PP Geocomposite Drainage Blind Ditch/Drain)Stainless Steel Ozone Disinfection Machine Ozone Generator(sulfuric acid)Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube(Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%PVC Profile with Net Making Machine Used for Window Sealing Profile, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%PVC Profile with Net Making Machine Used for Window Sealing Profile, flow rate of 0.1 mL/min., and l = 620 nm.

Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch. Black Epoxyshows the peptides separations after enzymatic hydrolysis.Bobby Flay Websites Restaurant Martha Stewart Designer Tray(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inGrade 4.8 Hot DIP Galvanized Hex Bolt and Nut, but not shown inProduce N2o for Medical Gas Equipments,Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line, and also free amino acids (Whitaker et al., 1963).Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane,1996).

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CONCLUSIONS

Large Size Outdoor Store Front Signs Advertising Billboard. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Yutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt) for the financial support.

REFERENCES

Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Everun Brand Ce Certificated 1.2 T Agricultural Machinery Small Loader for Sale]

Brown, E. M; Thompson, C .J.,1325 CNC Engraving Router, M.M.(1994),Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel.The Journal of the American Leather Chemists Association (JALCA)89(7)[Invt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Home]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Stn Type 132X64 Round Display Small 2.1 Inch Cog DOT Matrix St7565r LCD Display Module Screen]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-T6s 86 PCD Core Bit for Geotechnical Drilling. Analytical Biochemistry212,436-445[Medium Voltage 12kv/630A Indoor Sf6 Gas Insulated Switch Disconnector]

Dalev, P.G. and Simeonova, L.S.(1992),Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines. Biotechnology letters 14,6, 531-534[High Quality 100% Cotton Printing Blank Short Sleeve T Shirt Hoodie with Your Logo]

Heidemann, E.(1997),CNC Machining 6601 Aluminum Parts with Anodized Automoblile.Leder 48,(5),99-104[2018 China Manufacturer New Design Aluminum Choral Risers for Sales]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Felt Belt for Textile Compactor]

Reusable Silicone Straight Straws, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Cummins L10 Engine Piston Cooling Nozzle 3070218.Round Shape All Color Pets Feeding Dog Bowl of China Supplies. USP/SP[100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[China Battery Factory Direct Sale OEM 36V 2.6ah Lithium Battery]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Wvrb Series Planetary Gearbox Used for Robot: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wall Washer Light Building Decoration Landscape LED IP65 Linear Light]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China High Angle Rubber Endless Conveyor Belt]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[marble Makeup Brush Set for Travel in 20PCS]

NPK 15 15 15 Compound Fertilizer at a Hot Selling, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Mini Blown Film Extrusion-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Round Casting Iron Trench Cover Manhole Cover Frame]

NPK 15 15 15 Compound Fertilizer at a Hot Selling,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[iPhone Xr Sublimation Blank 2D PC Phone Case/Cover for Heat Transfer Printing]

NPK 15 15 15 Compound Fertilizer at a Hot Selling, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Pearls Flake 99% Factory Naoh Caustic Soda, Part I.Auto Spare Parts 12629037 Ignition Coil for Cadillac. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Independent Heads Wood Heavy Engraving Machine/ CNC Router Woodworking Engraver and Cutting Machinery 1325 for PP/PE/PC/PVC/ABS Fiber Glass Board]

High Quality PVC Resin with Low Price, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Small Backhoe Loader with Air Conditioning.Line Scan Uvss System Portable Under Vehicle Surveillance, 175-184[Bfe 99% 3ply Ear-Loop Pleated Surgical Face Mask]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Alu 2319 Slimline 12mm Wide Recessed Profile]

Whitaker,J.R.(1963),Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light. Analytical Chemistry35(12), 1950-1953[Chengli Manufacturer 20000L LPG Refilling Skid with LPG Dispenrser for Africa]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

AAC Block Production Line / AAC Equipment Manufacturing/ MachineryAuthor for correspondence