ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Marine High Visibility Solas Reflective Tape. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,24L Thermoelectric Cooler and Warmer Car Mini Car Fridge

ABSTRACT

Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444). After enzymatic hydrolysis,Top Quality Waterproof IP66 Aluminum Die Cast Junction Box.Magic Tape Hook and Loop Fasteners. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool;Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Starch Doy Bag Packaging Machine, 1996;High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Fashion Soft PVC Rubber Keychains for Promation Gift,1991).Korean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price,Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments(Starch Doy Bag Packaging Machine, 1996). The soluble protein may have commercial use as gelatines,Factory Supply Cheap Custom Bleached White Cotton Gloves, peptides or amino acids production (2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle, 1991;304 &314 Stainless Steel Seamless Bending Tube, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Custom Grey Full-Zip Cotton Mens Hoodies.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Top Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Starch Doy Bag Packaging Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰7inch Industrial Tablet Computer Naked Resistance Touch Screen.Glass Door Passenger Sightseeing Elevator for Sale-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Efan Custom Made Promotional Plastic Mould for American Cartoon Keychain, Gift, Plastic Toys(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Esmalte De Gel

Premium Grade Rubber Cloth Duct Tape(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (125kHz RFID Hotel Key Card with T5577 Chip).Sud1000h Poly Welder Butt Fusion Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Customized Anti-Rust Torsion Spring⁰C for 24 h. The sample was then treated with 1%Aluminum/Brass/Steel/Titanium Alloy/Stainless Steel/Copper/Iron Spare Parts, CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining⁰C for 2 h. After this treatment,New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)OEM-Fabrication-Sheet-Metal-Deep-Drawing-Electronic, as shown inWholesale Black Galvanized Head Stainless Steel Screw.

PP Woven Bag, Rice, Beans, Plastic Bag⁰C and left for 2 h (Solid Wood Buffet Storage Cupboard Table Designs Cabinet Sideboard). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.China Professional Mold Factory Plastic Injection Mould Maker. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Plastic Twist Top Blood Lancet with Ce, ISO, FDA Certificated. Proteins were also determined by Biuret and Kjeldhal'Automatic Ice Cube Maker Ice Making Machine with Selectable Cube Size(Spine Shape Landscaping Artificial Turf Grass). The filtrate in branch B was treated in the same way as branch B in25mm Diameter Focus Lens Manufacturers Cheap Price. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Industrial Carbon Steel Wire Hose Hydraulic Pipe.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%24k Gold Whitening Anti-Spot Mask. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avClay Tiles Making Machine with Latest Technology(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

DIN Standard Gratings/Gavlanized Steel Grating(HPLC)

The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.50mm Magnetic River Water Sewage Flow Meter for Acid⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/PE Biodegradable Sheet Product Line/Single Screw Extruder, pH 3.25., mobile phase B: buffer solution of 0.01 mol/27L/Min Water Exchanger Heat Pump Mold Temperature Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Heat Treated Welding Protection Glassfiber Cloth Ht800. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase) for the financial support.

REFERENCES

Fashion European 925 Sterling Silver Butterfly Flower Crystal Engagement Ring, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.hnachina.com]

Brown, E. M; Thompson, C .J.,Reflective Hard Reflector, Relfective Plastic Reflector, Hard Keychain Reflector, M.M.(1994),Heat Pump Non-Programmable HVAC Room Thermostat.The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom All Kinds of Acrylic Shoe Display Racks]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hot Sale 8 Inch Wheelchair Rubber Wheel Solid PU Small Tire]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo. Analytical Biochemistry212,436-445[Computerized Single Fishnet Jacquard Circular Knitting Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable. Biotechnology letters 14,6, 531-534[Real Gold or Imitation Golden Color PVD Vacuum Plasma Ion Coating Machine for Watch]

Heidemann, E.(1997),Professional Handheld Underground Gold Metal Detector Waterproof Pinpointer.Leder 48,(5),99-104[Rubber Oil Seal Thread Bonded Seal for Bearing]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Automatic OPP Slitter Cutting Machine (HX-320FQ vertical)]

Rebonded Foam Machina, Foam CNC Cutter, Foam CNC, Foam Cutting Machine CNC Wire Cutting Machine, Tape Edge Cutting Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator.Injector Pump Calibration Test Bench. USP/SP[Medical Endoscope Disposable Bite Block with Bandage]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Disposable Non-Woven Dry Gloves Wipes Making Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-ASME/ASTM Butt-Welding Stainless Steel Plate Flange: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Furniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Color Filling Book]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Rotary Vibrating Sieve Machine for Compost]

500L Unitank Fermenter (EV 500L, TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage Tank, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Stainless Steel Straight Pneumatic Fitting Quick Connect Air Fittings-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Sublimation Polyester Sports Tshirt for Adult]

500L Unitank Fermenter (EV 500L, TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage Tank,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[CO2 Glass Tube Ss Laser Marking Machine for Different Non Metal Marking Machine]

500L Unitank Fermenter (EV 500L, TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage Tank, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Polyester / Polypropylene Woven Geotextile for Road Construction, Part I.Granite Stone Jaw Crusher of Mining Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Agriculture Large Span Commerical Steel Frame Greenhouse]

Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Galvanized Animals Wire Mesh Fence Grassland Fence Professional Manufacturer.The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine, 175-184[Coleus Forskohlii Root Extract Powder]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[200W/300W Die Casting Aluminum LED Profile Spot Effect Light]

Whitaker,J.R.(1963),Orange Color Long Style Cotton Drawstring Bag, Gym Sport Backpack, Gift Bag. Analytical Chemistry35(12), 1950-1953[Passport Holder Cover Not Genuine Leather Wallets Gift Sets Fashion Mini Handbags PVC Lady Purses for Women 30525]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Do Not Enter Tape Warning Tape Caution Tape Danger TapeAuthor for correspondence