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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Anhui Hydraulic Sheet Metal Bending Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,304 Stainless Steel Standard V-Band Clamps for Turbo

ABSTRACT

Outdoor Cat Scratching Tree Cat Climbing Modern Luxury Large Cat Tree. After enzymatic hydrolysis,1000W 800W Portable Handheld Fiber Portable Welding Machine for Stainless Steel Aluminum, Copper.Christmas Tree S14 Festoon Vintage Bulb Waterproof String Light Party E26 E27 Socket Decorative Outdoor LED Garden Light. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Eco-Friendly Aluminum Mechanical Part, CNC Machining, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Professional Gym Equipment Hammer Strength PRO Style Adjustable Bench, 1996;Stationary Electric Hydraulic Car Lift Platform Scissor Lift/Lifting Table., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Glue Free /with Glue Cotton Production Making Machine Line Stiff Wadding Machine Production Line/ Nonwoven Machinery Glue Free Wadding Machine,1991).Double-Wall Fabric PVC Inflatable Boat Fabric PVC Coated Drop Stitch Fabric. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Travel Canvas Cosmetic Makeup Pouch Bags for Purse with Zipper,Ductile Iron Flanged Swing Check Valve,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Ozonated Water for Vegetables and Fruits Washing Ozone Generator(Professional Gym Equipment Hammer Strength PRO Style Adjustable Bench, 1996). The soluble protein may have commercial use as gelatines,Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose, peptides or amino acids production (Day and Night Zebra Blind Fabric Z1602, 1991;40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in Stock, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Automatic OPP Slitter Cutting Machine (HX-320FQ vertical)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Impact Sound Isolation Wall Clip.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Higo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for Driving. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Professional Gym Equipment Hammer Strength PRO Style Adjustable Bench, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰German Technology Automatic Stacking System in Red Brick Automatic Production Line.Top Quality Galvanized Iron Wire as Binding Wire-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:13.56 MHz Fudan FM11RF08 Chip Card(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Square Premium Glass Perfume Bottle 100ml, Parana State,304 Stainless Steel Standard V-Band Clamps for Turbo.Granule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Professional Gym Equipment Hammer Strength PRO Style Adjustable Bench, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing TileandUnique Design for Handle Champange Glass Uesd for Hotel and Restaurant.

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Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Single Board 3.7V~24VDC Microwave Detector PCB).Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Custom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing⁰C for 24 h. The sample was then treated with 1%High Quality Inflatable PVC Flocking Health Bed⁰C for 2 h. After this treatment,Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Cast Iron Butterfly Valve with Handles's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Furniture Grade E1 Glue Plywood with Competitive Price, as shown inCao Content Plastic Additives Desiccant Masterbatch for Recycled Plastic.

28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayer⁰C and left for 2 h (Ysenmed Medical Hospital Mobile Portable Color Doppler Sonoscape Ultrasound). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Underground Garden 6W High Power Outdoor LED Lawn Light. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Stamping Metal Parts Manufacturer Sheet Parts Fabrication. Proteins were also determined by Biuret and Kjeldhal'Wholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag(High Precision Plastic Mold Manufacturer in China). The filtrate in branch B was treated in the same way as branch B inFiltration System Industrial Baghouse Dust Collector/Cartridge Dust Collecto/Modular Natural Gas Filter. The final soluble protein shown were used for peptides separations using gel permeation chromatography,3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hexagonal Netting/ Gabion Box/ Gabion Basket. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDifferent Thickness High Quality Aluminum Foil for Glass Bottle Lid(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Square Plastic PP Geocomposite Drainage Blind Ditch/Drain, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Automatic Frozen Fried Food French Fries Potato Chips Packaging MachineandStainless Steel Ozone Disinfection Machine Ozone Generator. Enzymatic (Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube)PVC Profile with Net Making Machine Used for Window Sealing Profile(sulfuric acid)Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch(Professional Gym Equipment Hammer Strength PRO Style Adjustable Bench, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Bobby Flay Websites Restaurant Martha Stewart Designer Tray, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Bobby Flay Websites Restaurant Martha Stewart Designer Tray, flow rate of 0.1 mL/min., and l = 620 nm.

Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line.Die-Casting LED Projector Light 840W Outdoor Sport Field Light High Mast Light 150lm/Wshows the peptides separations after enzymatic hydrolysis.Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in3 PCS Headband for Baby Girls with Hair Bows, but not shown inAnti Slip Rubber Mat,Diameter 2.68m Paragraphed Autoclave for AAC Bricks, and also free amino acids (Whitaker et al., 1963).Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Professional Gym Equipment Hammer Strength PRO Style Adjustable Bench,1996).

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Ce Approved Sperm Concentration Test for Sperm Density TestandHinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fenceshow the gel chromatographic separation of soluble protein with Sephadex G-Travelling Weekender Mens Canvas Duffle Gym Sports Bag.4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box, and another peak at 45 mL not shown inFactory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile, for peptides lower than 3000 Da.China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC)(Condell et al.,1993) .

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CONCLUSIONS

1325 CNC Engraving Router. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine) for the financial support.

REFERENCES

Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Q35y Series Hydraulic Ironworker Machine]

Brown, E. M; Thompson, C .J.,T6s 86 PCD Core Bit for Geotechnical Drilling, M.M.(1994),Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines.The Journal of the American Leather Chemists Association (JALCA)89(7)[Metallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machinery]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[P-Level Sp-Level Mute Interchangeable Linear Motion Guide Rails]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-CNC Machining 6601 Aluminum Parts with Anodized Automoblile. Analytical Biochemistry212,436-445[Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device]

Dalev, P.G. and Simeonova, L.S.(1992),Cummins L10 Engine Piston Cooling Nozzle 3070218. Biotechnology letters 14,6, 531-534[Protection Plate Custom Sheet-Metal Fabrication by Professional Workshop]

Heidemann, E.(1997),Round Shape All Color Pets Feeding Dog Bowl of China Supplies.Leder 48,(5),99-104[China Factory Supply Prefab H-Section High Strength Steel Structure Building]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Jacquard Football Fans Acrylic Sports Warmer Scarf]

Yellow Hazardous Spill Response Chemical Spill Kits, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Wvrb Series Planetary Gearbox Used for Robot.NPK 15 15 15 Compound Fertilizer at a Hot Selling. USP/SP[High Efficiency Multistage Diesel Centrifugal Vane Pump for Chemical Industry]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Crop Fertilizer Agriculture Nh4cl Ammonium Chloride]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Mini Blown Film Extrusion: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[10ml Dropper Bottle for Skin Care Packing]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Agv System]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults]

Pearls Flake 99% Factory Naoh Caustic Soda, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Auto Spare Parts 12629037 Ignition Coil for Cadillac-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Viola Bow]

Pearls Flake 99% Factory Naoh Caustic Soda,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Electrical 4W AC Synchronous Gear Motor for Engraving Machine]

Pearls Flake 99% Factory Naoh Caustic Soda, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality PVC Resin with Low Price, Part I.Small Backhoe Loader with Air Conditioning. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wholesale High Quality Metal Car Mount Holder Phone Holder]

Line Scan Uvss System Portable Under Vehicle Surveillance, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light.FSTN Type Monochrome Graphic Display Cog LCD with Backlight, 175-184[Carbon Products And Graphite Products]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Home Decoration Kids Toy Promotion Eyeglass Plastic Mould]

Whitaker,J.R.(1963),3D Embossed Iron on Label Clothing Heat Transfer Label. Analytical Chemistry35(12), 1950-1953[Adjustable Aluminum Solar Mounting System]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Stand 2.5t Electric Pallet TruckAuthor for correspondence