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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Vertical Automatic Granule Bag Packing Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Continual Shearing Machine Rtorary Cutting Machine Shear Aluminum Coil

ABSTRACT

160lm/W Die-Casting Aluminum UFO 150W LED High Bay Light Fixtures. After enzymatic hydrolysis,4000W Industrial High Power Pipe Plate Whole Cover Exchange Platform Metal Fiber Laser Cutter for Carbon/Aluminum/Stainless/Brass 3015gr.Food Additives 99% Aspartame (APM) CAS 22839-47-0 with Best Price. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Small Manufacturing Tht/ Ai Double Wave Soldering Machine;Manual Sealing Machine for Instant Noodles Paper Soup Bowls; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Dongfeng 153 Type 10m3 12m3 Arm Roll Garbage Truck, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wholesale Custom Fashion Men Sunglasses Bifocal Sports Sunglasses for Reading, 1996;190t Waterproof Outdoor Foam Foldable Stadium Seat Cushion., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Corrugated Steel Sheet Corrugated Steel Roofing Sheet,1991).China Factory Queen Mattress with Visco Memory Foam and Latex. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing,Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Customized Hot Forging Agricultural Spare Parts(Wholesale Custom Fashion Men Sunglasses Bifocal Sports Sunglasses for Reading, 1996). The soluble protein may have commercial use as gelatines,16mm 12 Strand UHMWPE Synthetic Fiber Rope, peptides or amino acids production (High Quality Customized Detachable Grid Logistics Storage Industrial Racking Systems, 1991;Vertical Type Caseformer Machine WJ-LKX-15, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Fb-5.0s 5.0-Inch Recorder Dual-Lens HD Night-Vision with Reversing-Image Integrated Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Professional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mould.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-0.1mm Tungsten Carbide 2f Single Flute Micro End Mill. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wholesale Custom Fashion Men Sunglasses Bifocal Sports Sunglasses for Reading, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Agriculture Large Span Commerical Steel Frame Greenhouse.Ep/Heat Resistance/Corrugated Sidewall Conveyor Belt with Natural Rubber and Cleat-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Factory Price Printing Doy Pack Bag(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (H. 265 2.0megapixel 4X Zoom (2.8-12mm) IP IR Waterproof Camera).Teaching Equipment Laminar Flow Visualization Unit Fluid Mechanics Lab Equipment Vocational Training Equipment(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%New Design 4*2 Street Cleaning Machine Sweeping Truck with Brushes⁰C for 24 h. The sample was then treated with 1%Direct Factory Tebuconazole Price Fao Bactericide Tebuconazole Price⁰C for 2 h. After this treatment,High Quality Plastic Pipe Adapter HDPE100 Pipe Fitting Flange Adapter PE Butt Fusion Pipe Flange Adapter for Water Supply SDR17. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Engine Parts Rubber High Quality Timing Belt 111ru25's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wholesale Interior Metal 3D Bee Garden Ornament Decoration, as shown inBosch Fuel Injector for Weichai Cummins Komatsu Engine 0445120123 Vg1038080007 Vg1560080276 095000-8011.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fish Scales Texture Fashion Leather for Shoe (S312100GH). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDiamond Segments 2000mm Diameter Granite Cutting Blade(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hot Selling Furring Channel Fittings Suspended Ceiling Accessories⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/90m3 Per Hour Mobile Concrete Pump, pH 3.25., mobile phase B: buffer solution of 0.01 mol/China High Quality CATV FTTH 1550 Nm External Modulated Optical Transmitter, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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for Wall Decoration Glass Silver Metal Aluminium Mosaic Tile.I9s Tws Wireless Earphone Portable Bluetooth Headset Earbudshows the peptides separations after enzymatic hydrolysis.100% Polyester Gabardine Shirting Fabric with Transfer PrintingTree(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in450mm 500mm Diamond Saw Blade with Good Sharpness for Reinforced Concrete Cutting, but not shown inPCD Concrete Grinding Cup Wheel Diamond Tools for Urethane Epoxy and Paint,School Kid Sport Travel Backpack Fashion Casual Promotional Bag (GB#20056), and also free amino acids (Whitaker et al., 1963).Fuguang High Quality Fb1015-280 280ml Double Wall Glass Water Bottle. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Wholesale Custom Fashion Men Sunglasses Bifocal Sports Sunglasses for Reading,1996).

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CONCLUSIONS

Ms-184125-2 1.3mh Arnold Core Common Mode Choke Coils Toroidal Inductor. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer) for the financial support.

REFERENCES

Hot Sale Thermostat Electric Heating Element for Solar Water Heaters, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[21FT Aluminium Center Cabin Boat with 360 Walk Around]

Brown, E. M; Thompson, C .J.,Phillip Countersunk Head Screw Hot DIP Galvanized, M.M.(1994),Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse.The Journal of the American Leather Chemists Association (JALCA)89(7)[EPS/Rock Wool/Glass Wool/PU Sandwich Wall Board with High Quality Low Price]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Metal Window Screen]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Cow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll. Analytical Biochemistry212,436-445[High Quality Casting Cover Aluminum Die Casting Products]

Dalev, P.G. and Simeonova, L.S.(1992),Heat Shrink Automatic Flow Wrapping Packing Machine. Biotechnology letters 14,6, 531-534[PVC/PP/PE Twin Screw Plastic Granules Extruding Granulator Machine]

Heidemann, E.(1997),Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam.Leder 48,(5),99-104[Carbon Electrode Paste]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Food Grade Stainless Steel Milk Dairy Equipment for Factory]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Chinese Jbcz Handy Operation Threading Machine and Coupler]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Fashion Design and Comfortable Safety Work Shoes with Composite Toe]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[switchable smart film]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hot Filling 4-in-1 Fruit Juice with Pulp Making Bottling Machine]

5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality Sheet Metal Bending Roll Forming Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Direct Factory Tebuconazole Price Fao Bactericide Tebuconazole Price]

5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[China High Quality CATV FTTH 1550 Nm External Modulated Optical Transmitter]

5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Different Size Glass Test Tubes, High Quality Laboratory Test Tubes, Part I.Professional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[No Toxic Lead Free Hardness Surface PVC Foam Board/Sheet]

Frame Type Rubber Hydraulic Press Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Kitchen Metal Dish Storage Rack (JP-SC984C).0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming, 175-184[China High Precision Anodized Aluminum CNC Machining Turning Parts]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China Factory Queen Mattress with Visco Memory Foam and Latex]

Whitaker,J.R.(1963),AVR Se350 Automatic Voltage Regulator for Marathon Alternator. Analytical Chemistry35(12), 1950-1953[9~12 Inch Mechanical Backing Plate]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Chinese Forklift Hydraulic Manual Fd30 3 Ton Forklift TruckAuthor for correspondence