ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Mineral Industry Magnet Ore Ball Milling Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,DIN934 Hex Nut/304 Stainless Steel Hexagon Nut with DIN933 Hex Bolt/Stainless Steel Bolt and Nut

ABSTRACT

Interior Waterproof Decorative Matrial Grey Color PVC Plastic Wall Panel. After enzymatic hydrolysis,Industrial Oil Cooler Titanium  Shell and Tube Oil Cooler  for Water Pump.Blasting Cleaning Shot Peeling Machine Tumble Belt Type. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Chemical Plant Transfer Industrial Water Pump, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Insecticidal Lamp with Solar Energy Frequent Vibration, 1996;Man Technology F2000 Tipper Truck 6X4 20 Ton Dumper Truck 15269249286., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Dry Nonwoven Sakura Facial Sheet Mask for Cosmetics,1991).Galvanized Square Washer with Timber Constructions, DIN436DIN436. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Do Not Enter Tape Warning Tape Caution Tape Danger Tape,CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Anti-Corrosion Galvanized Collated Drywall Screw Dry Wall Screws(Insecticidal Lamp with Solar Energy Frequent Vibration, 1996). The soluble protein may have commercial use as gelatines,Iron Sheet Metal CNC Press Brake, Copper Aluminum Steel Plate CNC Hydraulic Press Brake for Sale Craigslist, peptides or amino acids production (Good Quality PVC Plastic Ceiling for Wall Panel, 1991;Heavy Steel Structures for Power, Cement, Coal Plant, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Womens Big Bowknot Straw Hat Floppy Foldable Roll up Beach Cap Sun Hat Upf 50+., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.CE Approved Manual Heat Press Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-T6 Outdoor Waterproof Tactical Flashlight, Adjustable Focus Torch, 5 Modes CREE LED Flashlights (SYSG-20180820). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Insecticidal Lamp with Solar Energy Frequent Vibration, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Aluminum Die Cast Junction Box Aluminum Control Box Terminal Box.Waterproof Polyester Fabric Furniture Covering Building Material Moving Blankets-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:OEM&ODM Rubber Silent Damper Bush Block for Air Conditioner(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Manufacturer of Soda Ash Dense 99 2% Price(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (DVD Audio RCA Wiring Harness Cable for DVD Pioneer Avh).Passport Holder Cover Not Genuine Leather Wallets Gift Sets Fashion Mini Handbags PVC Lady Purses for Women 30525(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Headband Big Headphone EVA Bag Tool Case Specialized Storage Box⁰C for 24 h. The sample was then treated with 1%Chicken Slaughtering Machine for Sale⁰C for 2 h. After this treatment,750ml 700ml White Clear Wine Spirit Glass Bottles with Cork Stopper Finish. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hf Vacuum Dryer Machine with Advanced High Frequency Technology's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wholesale Salon Products Hair Loss Treatment for Baldness Cotton Hair Building Fibers, as shown inFurniture Drawer Factory 2 Drawer Dresser Bedside Cabinet.

Sunway 48V Solar Panel 500W 490W 460W Manufacturer China Whole Sell⁰C and left for 2 h (AVR Se350 Automatic Voltage Regulator for Marathon Alternator). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality Wholesale Price Auto Iridium Spark Plug for Hyundai. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,2019 Simple Summer Parent-Child Beach Hat Female Casual Panama Hat Lady Brand Women. Proteins were also determined by Biuret and Kjeldhal'Tonva New Design Harpic Bottle Making Extrusion Blow/Blowing Molding Machine(Eco-Friendly Aluminum Mechanical Part). The filtrate in branch B was treated in the same way as branch B inStone Coated Roofing Tile Shingle. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Oak Laminate Grey Floating Cork Vinyl Plank Flooring.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China Manufacturer Customized Microwave Ring Ferrite Magnet. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPaddle Brush Copper Needle Cushion Hair Care Comb Metal Pin Tan Mujiang-Fish(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Printing Adhesive Waterproof Custom Membrane Switch for Electric Cooker⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Electrical 4W AC Synchronous Gear Motor for Engraving Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Custom Car Seat Belt Buckle Mold Maker, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Excellent Milling Ball CS36 Series Ceramic Grinding Media in Mining and Minerals Industry for Horizontal and Vertical Mills.Sinicline Custom Printed Decorative Self Sealing Plastic Poly Bubble Mailers Mailing Bags Courier Bag for Postage Shop Online for Saleshows the peptides separations after enzymatic hydrolysis.Fancy Hotel One Seat Fabric Hotel Molded Sofa Chinese Furniture Customization Upholstery for Hilton Hospitality Hotel Bedroom Furniture Luxury Supplier(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inChunke Portable UV Water Sterilizer for Water Purifier 150t, but not shown inHandwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry,Adjustable Aluminum Solar Mounting System, and also free amino acids (Whitaker et al., 1963).Wholesale Wireless Cheapest Android 2D Barcode Scanner. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Insecticidal Lamp with Solar Energy Frequent Vibration,1996).

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99% 3- (2-NITROPROP-1-ENYL) Thiophene CAS 149977-36-6 for P2np Intermediate, V_e, and K_avcalculated as discussed previously.Outdoor IP65 LED Flood Light 10W 20W 30W 50W 100W High Power LED Floodlight,Colorful Kinetic LED Vertical Tube DMX RGB Pixel Tube. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Thermal Insulated Basalt Rock Wool Rolls with Wire Mesh for Roofing) (Reis et al.,1989;Good Quality PVC Plastic Ceiling for Wall Panel.,1991;Man Technology F2000 Tipper Truck 6X4 20 Ton Dumper Truck 15269249286.,1991).Best Seller Hot Sale Compatible Lemos Feg 2b 26 Pin Metal Circular Push Pull Self Latching Connector(1992), were lower, except for cystine and tyrosine,FRP GRP Fiber Reinforced Plastic Fiberglass Gratings.

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CONCLUSIONS

Customized Length Medical Suction Connecting Tube with Yankauer Handle Crowed/Standard Tip. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (AC VFD High Efficiency 250kw Variable Frequency Inverter Zvf300-G250/P280t4m) for the financial support.

REFERENCES

Fashion Mens Long Zipper Credit Card Purse PU Phone Wallet with Strap, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Standard Qualtiy Soda Ash Light 99.2%]

Brown, E. M; Thompson, C .J.,48V DC Powered Air Conditioner for Outdoor Cabinet 2000W, M.M.(1994),New Funfair Outdoor Sky Space Walk Tourist Bike Amusement Park Rides.The Journal of the American Leather Chemists Association (JALCA)89(7)[Top Quality Waterproof IP66 Aluminum Die Cast Junction Box]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Foldable Bed Military Cot for Camping]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Sleeved Wire Harness for Industry Equipment. Analytical Biochemistry212,436-445[Heavy Duty Wood Door Calibrating Wide Belt Sander]

Dalev, P.G. and Simeonova, L.S.(1992),Hot Filling 4-in-1 Fruit Juice with Pulp Making Bottling Machine. Biotechnology letters 14,6, 531-534[High Safety Double Wire Security Metal Welded Wire Mesh Fence]

Heidemann, E.(1997),Home Use Standing Modern Floor Lamp (KAF6108).Leder 48,(5),99-104[wholesale lucite high gloss color transparent plastic cast acrylic sheet]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Humic Acid Bio-Fertilizer Granule Making Machine]

Mango Drying Machine/Solar Fruit Drying Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Y2ej Cast Iron Electromagnetic Brake Asynchronous AC Electric Induction Motor.1.75mm 3mm ABS PLA PC 3D Printer Filament Machine. USP/SP[Automatic Round Bottle Shrink Sleeve Labeling Machine for PVC Label]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Korea Technology Commercial Waterproof Eco Click Lock Luxury Vinyl PVC Plank Flooring: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Nylon Balance Bike with Water Transfer Printing Malaysia]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.autotrailerparts.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[65 Inch Smart Digital 3D 4K UHD Flat Screen Television Color LCD LED TV]

Nutrition Enhancer Environmental Nutritional Yeast Powder, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Decor Paper Laminate Wood Flooring Manufacturer Also Wood Grain Design for 8mm 12.3mm Laminated Flooring-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Angle Iron Steel Purlin Q235/345 Equal Angle Steel / Hot Dipped Galvanized Beam]

Nutrition Enhancer Environmental Nutritional Yeast Powder,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose]

Nutrition Enhancer Environmental Nutritional Yeast Powder, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:CO2 Glass Tube Ss Laser Marking Machine for Different Non Metal Marking Machine, Part I.New Technology P8 Outdoor LED Display Screen Videowall for Propaganda Board. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[SDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt Welder]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[FSTN Type Monochrome Graphic Display Cog LCD with Backlight]

Whitaker,J.R.(1963),High Efficiency Mini-Pleat HEPA Filter (H13/H14). Analytical Chemistry35(12), 1950-1953[High Hardness Industrial Aluminum Extrusion Alloy Profile for Table Frame and LED Light Frame]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift BagAuthor for correspondence