ARTICLES

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ABSTRACT

Customized Stable Burn-in Test Equipment. After enzymatic hydrolysis,Wholesale Custom 100% Cotton Boys T-Shirt with Print.Industry 0.002mm Accuracy Checking Fixture. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Six Stations Pen Press Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (FDA Approved Cryo + RF Best Ultrasound Radio Frequency RF Slimming 80K Cavitation Slimming Machine, 1996;Vertical Automatic Granule Bag Packing Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Continual Shearing Machine Rtorary Cutting Machine Shear Aluminum Coil,1991).160lm/W Die-Casting Aluminum UFO 150W LED High Bay Light Fixtures. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,4000W Industrial High Power Pipe Plate Whole Cover Exchange Platform Metal Fiber Laser Cutter for Carbon/Aluminum/Stainless/Brass 3015gr,Food Additives 99% Aspartame (APM) CAS 22839-47-0 with Best Price,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Small Manufacturing Tht/ Ai Double Wave Soldering Machine(FDA Approved Cryo + RF Best Ultrasound Radio Frequency RF Slimming 80K Cavitation Slimming Machine, 1996). The soluble protein may have commercial use as gelatines,Manual Sealing Machine for Instant Noodles Paper Soup Bowls, peptides or amino acids production (Dongfeng 153 Type 10m3 12m3 Arm Roll Garbage Truck, 1991;Wholesale Custom Fashion Men Sunglasses Bifocal Sports Sunglasses for Reading, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;190t Waterproof Outdoor Foam Foldable Stadium Seat Cushion., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Corrugated Steel Sheet Corrugated Steel Roofing Sheet.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China Factory Queen Mattress with Visco Memory Foam and Latex. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (FDA Approved Cryo + RF Best Ultrasound Radio Frequency RF Slimming 80K Cavitation Slimming Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing.Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Customized Hot Forging Agricultural Spare Parts(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Agriculture Large Span Commerical Steel Frame Greenhouse(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Side Pack Manual Gas Engine Power Brush Cutter).Ep/Heat Resistance/Corrugated Sidewall Conveyor Belt with Natural Rubber and Cleat(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags⁰C for 24 h. The sample was then treated with 1%Ss400 Corner Metal Sheet Bar Galvanized Angle Iron⁰C for 2 h. After this treatment,High Speed Automatic Multi Rolls Toilet Paper and Kitchen Towel Packing Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Chinese Jbcz Handy Operation Threading Machine and Coupler, as shown inStationery PP Clipboard With 7 Inch Scale Ruler.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Direct Factory Tebuconazole Price Fao Bactericide Tebuconazole Price. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Plastic Pipe Adapter HDPE100 Pipe Fitting Flange Adapter PE Butt Fusion Pipe Flange Adapter for Water Supply SDR17(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wholesale Interior Metal 3D Bee Garden Ornament Decoration⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Heavy Duty 90 Degree Large Decorative Table Iron Metal Stainless Steel Adjustable Angle Shelf Wall Mounted Metal Table Folding Bracket, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Disposable Non-Woven Dry Gloves Wipes Making Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Fuguang High Quality Fb1015-280 280ml Double Wall Glass Water Bottle. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Vertical Type Caseformer Machine WJ-LKX-15) for the financial support.

REFERENCES

Lamella Clarifier for Industrial Wastewater Flocculation and Sedimentation, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Brand Minyu with Gyratory Crusher (WLCC1300)]

Brown, E. M; Thompson, C .J.,0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machine, M.M.(1994),125kHz RFID Hotel Key Card with T5577 Chip.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Aerospace Equipment]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Promotional Jewelry Gift Paper Box with Ribbon and Inner Tray]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-USB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office Plug. Analytical Biochemistry212,436-445[BS1387 Class B 2 Inch Sch40 Gi Pipe Galvanized]

Dalev, P.G. and Simeonova, L.S.(1992),Fashion Large Capacity Transparent PVC Leather Handbag Tote Shopping Bag. Biotechnology letters 14,6, 531-534[Shima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Condition]

Heidemann, E.(1997),Crop Fertilizer Agriculture Nh4cl Ammonium Chloride.Leder 48,(5),99-104[China Movable Hydraulic Auto Scissor Lift]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ LED Bayomet Vehicle Lamps]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Automatic CNC Glass Cutting Machine Glass Cutting Table]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Ms-184125-2 1.3mh Arnold Core Common Mode Choke Coils Toroidal Inductor: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Top Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China 8layer Hdi Pcb]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901)]

Hot Sale Thermostat Electric Heating Element for Solar Water Heaters, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Phillip Countersunk Head Screw Hot DIP Galvanized-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Industrial 30W/50W/80W/100W/120W/150W/200W/300W Remote Control LED Solar Street Flood Lawn Gardern Wall Solar Light]

Hot Sale Thermostat Electric Heating Element for Solar Water Heaters,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[18X16mesh Fiberglass Window Screen Insect Screen]

Hot Sale Thermostat Electric Heating Element for Solar Water Heaters, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse, Part I.Cow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap]

Heat Shrink Automatic Flow Wrapping Packing Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam.Ductile Iron Standard Brackets Pipe Hanger, 175-184[Decoration Drywall Material Stud Track Light Steel Keel]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[FRP Counter Flow Low Noise Round Type Cooling Tower 600rt]

Whitaker,J.R.(1963),Luxury Vintage Throw Pillows Cover for Couch, Damask Pillows Cover, Decorative Pillow Covers. Analytical Chemistry35(12), 1950-1953[Pipe Conveyor Belt]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Hydraulic Orbital Valve Power SteeringAuthor for correspondence