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ABSTRACT

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Key words;High Quality Ceramic Chrome Hybrid Bearing Oil;Medical Product Nonwoven Disposable Surgical Gown PP Isolation Gown; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Digger Arm Small Wheel Loader (HQ908) with CE, 1996;15ml 18ml Glass Fish Model Perfume Bottle with Plastic Sprayer., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output,1991).Water Based Emulsion Paint Production Line. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Cnm Single Strand Anchor Coupler,Wisdom LED Floor Lamp, Head Lamp Fabia Mining Lamp,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,18X16mesh Fiberglass Window Screen Insect Screen(Digger Arm Small Wheel Loader (HQ908) with CE, 1996). The soluble protein may have commercial use as gelatines,Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch, peptides or amino acids production (Sterile Dsiposable Syringe for Dental 12ml, 1991;Foldable Bed Military Cot for Camping, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;P5 960*960mm LED Display Screen SMD Indoor /Outdoor., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.2019 New Advertising Inflatables Air Walking Backpack Ball.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Side Pack Manual Gas Engine Power Brush Cutter. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Digger Arm Small Wheel Loader (HQ908) with CE, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Punching Bending Welding Steel Work Fabrication.Black and White Cat Mobile/Cell Phone Cover/Case for iPhone 6/7/6plus/7plus-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Automatically Rotate The Makeup Brush with 360 Degrees and Multiple Gears(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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180W 1850mm*80mm Reci Laser Tube(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine).OEM Factory Balloon Padding Textile Machinery(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%NdFeB Cylindrical Magnets Coated with Holes  Zn, Ni, Black Epoxy⁰C for 24 h. The sample was then treated with 1%Kids Thermal Insulated Bento Bag Children Picnic Lunch Cooler Bag (BC0089)⁰C for 2 h. After this treatment,Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Outdoor Concert Stage Equipment Aluminum Stage Portable Stage's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Invt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Home, as shown inPTFE Liner Hastelloy Alloy Electrode Electromagnetic Flow Meter.

22*30mm 12SMD 5050 LED Reading Light for Car⁰C and left for 2 h (China Wholesale Disposable Medical Consumable Safety Blood Lancet with Painless). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.SMS 25g Non Woven Isolation Gown with Knitted Cuff for Hospital. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness. Proteins were also determined by Biuret and Kjeldhal'Outdoor Cheap Portable Stage Aluminum Truss Stage(Automotive Acrylic Wholesale Masking Tape Factory Price). The filtrate in branch B was treated in the same way as branch B inrefractory magnesite alumina spinel. The final soluble protein shown were used for peptides separations using gel permeation chromatography,One Arm Motorized Multifunction Hospital Equipment for China Supply.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Custom Anodized Aluminum Bending Parts Sheet Metal Stamping Parts. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avP3.91 500X500mm Indoor Portbale Flexible LED Curtain Display(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Metallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machinery, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Printed Wristband Cotton Wristband RFID Woven Wristband, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Promotional Plain Silver Empty Hinged Rectangular Tin Box. Trailer RV Vision Securityshows the peptides separations after enzymatic hydrolysis.Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCustom Waterproof Pizza Sign Large Advertising Signboard, but not shown inFine Bubble Diffuser for Air Purifier,5 in 1 Swing Heat Press Machine (CAP, PLATE, MUG, T-SHIRT) Sublimation Transfer, and also free amino acids (Whitaker et al., 1963).Butyl Glue Self-Adhesive Tape with Radian, Caulk Strip Trim Tape (38mm). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Digger Arm Small Wheel Loader (HQ908) with CE,1996).

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CONCLUSIONS

Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce Approval. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Auto Plastic Part Hardware Plastic Injection Mold) for the financial support.

REFERENCES

High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[10ml Ultra-Q Hyaluronic Acid Buttocks Enlargement Injections]

Brown, E. M; Thompson, C .J.,10 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts Damper, M.M.(1994),300ton/H Mobile Sub Base Soil Mixing Plant for Road Construction.The Journal of the American Leather Chemists Association (JALCA)89(7)[Reusable Silicone Straight Straws]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Small Leather Shoes Craft Laser Cutting Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-BS 5163 Ductile Iron Rising Stem Gate Valve. Analytical Biochemistry212,436-445[1 Inch NPT Screwed Globe Valve]

Dalev, P.G. and Simeonova, L.S.(1992),CNC Aluminum Sheet Press Brake. Biotechnology letters 14,6, 531-534[Qt10-15 Automatic Concrete Cement Hollow Solid Paver Curbstone Brick Block Making Machine Price in Madagascar]

Heidemann, E.(1997),ID25mm Od86mm Standard Through Hole Slip Ring.Leder 48,(5),99-104[Follicle Stimulating Rapid Test Kit High Quality Supplier]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Antique Gold/Copper Coin/Bright Gold/Copper London Ride Medal]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Cow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Food Grade Silicone Baby Pacifier for Newborns]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Luxury Special Design Decorative Quality Gold Color Office Round LED Standing Floor Lamp (LT-11)]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Stainlesss Steel 304 Forged Pipe Nipple Threaded Nipple (KT0576)]

Platform Trolley with 150kg Capacity, 4 Castor Wheel, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Quality Shaper Machine Bc6085 Shapping Machine]

Platform Trolley with 150kg Capacity, 4 Castor Wheel,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery]

Platform Trolley with 150kg Capacity, 4 Castor Wheel, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V, Part I.All Size Colored Tapping Hex Head Self Drill Screw Wood Screw. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[16mm 12 Strand UHMWPE Synthetic Fiber Rope]

Pre-Engineered Cage Broiler Poultry Farm Building, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Alumina Ball with High Quality.PVC Textile Fabric for Outdoor Sports Waterproof Backpack, 175-184[Heavy Duty Rotating Display Stand Turntable]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Ozonated Water for Vegetables and Fruits Washing Ozone Generator]

Whitaker,J.R.(1963),White 4*8FT 18mm PVC Foam Board for Cabinets Use. Analytical Chemistry35(12), 1950-1953[Blue White Yellow Red Black Nylon Mosquito  Window Netting]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Concrete Aluminum Formwork System Aluminium FormworkAuthor for correspondence