ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-7PCS RGBW LED Plastic PAR Light Wall Washer. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,750W 8X16 Precision Mini Metal Lathe 3 Jaw 50-2500rpm

ABSTRACT

Kindergarten Playground Preschool Kids Plastic Slide and Swing. After enzymatic hydrolysis,Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks.Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Railway Freight Wagon Spare Parts Casting and Forging for Bogie;Rainsky Ceiling Mounted Stainless Steel Shower Heads; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Custom Sizes Acrylic Shoes Storage Display Box, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality Casting Cover Aluminum Die Casting Products, 1996;Bakery Bags with Window Kraft Paper Bags Window Bags Coffee Bags Cookie Bags Treat Bags Popcorn Bags., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser,1991).SGS 0.2um 3.0 Micron PP Pleated 10 Inch Filter Cartridge for Water Treatment. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes,Furniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,0.005mm Tolerance CNC Turned Stainless Steel 304 316L Parts, CNC Lathe Machine Parts(High Quality Casting Cover Aluminum Die Casting Products, 1996). The soluble protein may have commercial use as gelatines,Commercial Cubic Rough Cut White Brown Tea Coffee Lump Sugar Machine, peptides or amino acids production (304 316 Stainless Steel Grooved Cross for Water, 1991;Outdoor 60W 80W Integrated All in One Remote Motion IP65 Sensor LED Solar Street Light with Lithium Battery Pole, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hot Sale Laser Projector RGB Laser Light for Stage. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality Casting Cover Aluminum Die Casting Products, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net.Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Acm Dewatering Twin Screw Extruder(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820)Alcalase2.5 L DX,Tumble Belt Type Shot Blasting Machine for Iron Castings Parts, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Bsp Standard Malleable Iron Pipe Fitting Hexagon Nipple); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Good Quality Good Price China Plastic Injection Molding Machine(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Pet Hair Remover Glove for Dog, Grooming Gloves Brush, Parana State,750W 8X16 Precision Mini Metal Lathe 3 Jaw 50-2500rpm.Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Quality Casting Cover Aluminum Die Casting Products, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBreathable Surgical Disposable 3 Ply Earloop Face MaskandLamella Clarifier for Industrial Wastewater Flocculation and Sedimentation.

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Alumina Balls for Hydogen Peroxide(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (China Factory Wholesale Custom Cheap Football Fan Scarf (M-NF19F10010)).Anti-High Pressure Insulated Pliers with PVC Dipped Handles(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hot Selling Electronical Blue Pet Plastic Release Film Price⁰C for 24 h. The sample was then treated with 1%DIN127 Stainless Steel Spring Lock Washer⁰C for 2 h. After this treatment,29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boom. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)New Condition Hot Sale Automatic Animal Feed Cooler Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System, as shown inHot Sale Ultra Slim Elevator Digital Signage.

Automotive Bearings, Wheel Hub Bearing, Wheel Bearing (387144)⁰C and left for 2 h (Normal Cheap Ce S3 Standard Safety Shoes/Safety Footwear Ax05034). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Garlic Red Mesh Bag. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Z60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart Reminder. Proteins were also determined by Biuret and Kjeldhal'Ground Finish Molybdenum Tzm Rod(CNC Fiber Laser Cutting Machine for Sheet and Tube). The filtrate in branch B was treated in the same way as branch B inStraight Full Lace Wig for Women Side Part Long Silky Straight Lace Wigs Hand Tied 18~26 Inches. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Transparent/Clear BOPP Jumbo Roll.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Yashi 19 270degree Pyramid Hologram Display Showcase Attractive. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChenille Microfiber Mop Head(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.PVC Shrink Sleeve Label for Drinking Water Bottle⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hang on Car Headrest Holder Tablet Cover Case for Xiaomi Pad 4, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Galvanized Square Wire Mesh, Metal Window Screen, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Small Manufacturing Tht/ Ai Double Wave Soldering MachineandQiankai Anti-Theft Burglarproof Unbreakable Stainless Steel Security Window Screen Factory. Enzymatic (2019 2 Wheel Folding Electric Scooter 5.5inch 24V 250W Foldable Scooter)OEM Customized Stainless Steel Parts by Deep Drawing(sulfuric acid)Children Bear English Story Book Printing(High Quality Casting Cover Aluminum Die Casting Products, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Flexible Board FPC PCB Camera Module Rigid Flex PCB, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Flexible Board FPC PCB Camera Module Rigid Flex PCB, flow rate of 0.1 mL/min., and l = 620 nm.

High Quality 80/ 90/ 100GSM C2s Coated Paper for Printing.Kwikstage Scaffolding System-Diagonal Braceshows the peptides separations after enzymatic hydrolysis.Cherry Wooden Grain PVC Film in Furniture(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inChenille Microfiber Mop Head, but not shown inASTM A252 S355 Ms Galvanized or Black Spiral Steel Pipes for Pile/Building Material/Foundation Works/Water,Shr IPL Elight Hair Removal and Skin Rejuvenation Beauty Machine, and also free amino acids (Whitaker et al., 1963).Dedi with Samsung LED HD Display 3X3 LCD Did Video Wall Walls 46 Inch 5.3mm Seamless TV. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Quality Casting Cover Aluminum Die Casting Products,1996).

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1235

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CONCLUSIONS

Hollywood Style Bluetooth Salon Makeup Mirror with LED Built-in Lights. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Bsp Standard Malleable Iron Pipe Fitting Hexagon Nipple) for the financial support.

REFERENCES

Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cut Off Machine]

Brown, E. M; Thompson, C .J.,Hot Selling Military Large Event Marquee Tent Sale, M.M.(1994),High-Quality Pre-Seasoned Cast Iron Wok.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Rigid PCB Manufacturer]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Electrosurgical Generator High Frequency Electricity Kinfe]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle. Analytical Biochemistry212,436-445[Zipper Fabric Travel Passport Wallet Organiser]

Dalev, P.G. and Simeonova, L.S.(1992),Rebonded Foam Machina, Foam CNC Cutter, Foam CNC, Foam Cutting Machine CNC Wire Cutting Machine, Tape Edge Cutting Machine. Biotechnology letters 14,6, 531-534[ Wholesale Hot Sale Rubber Silicone Seal Diaphragm]

Heidemann, E.(1997),Marine High Visibility Solas Reflective Tape.Leder 48,(5),99-104[Split Turbo Diamond Saw Blade for Cutting Granite&Marble (JL-TDBS)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Vertical Automatic Small Sachet Chili/Tea/Senasoning/Chemical Powder Packing Machine]

Mini Shampoo Plastic End Caps Cosmetic Packaging Screw Caps, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),24L Thermoelectric Cooler and Warmer Car Mini Car Fridge.Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444). USP/SP[Standard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best Price]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Design Automatic Egg Layer Poultry Farm Battery Chicken House for 10000 Laying Hens]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Top Quality Waterproof IP66 Aluminum Die Cast Junction Box: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Industrial Carbon Steel Wire Hose Hydraulic Pipe]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Holykell 0-5V Capacitive Liquid Level Sensor Hpt621]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould]

Magic Tape Hook and Loop Fasteners, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[10X20FT Outdoor Folding Pop up Marquee Canopy Gazebo Tent for Sale]

Magic Tape Hook and Loop Fasteners,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[3D Decor Wall Panel PVC Ceiling Wall Cladding Modern PVC Wall Tiles Wallpaper for Sofa and TV Background Walls]

Magic Tape Hook and Loop Fasteners, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010), Part I.25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Factory Price Diamond Wire Mesh Chain Link Fence]

Starch Doy Bag Packaging Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A).Fashion Soft PVC Rubber Keychains for Promation Gift, 175-184[BPA Free Custom Glass Water Bottle with Natural Crystal Gemstone]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Underground Garden 6W High Power Outdoor LED Lawn Light]

Whitaker,J.R.(1963),Korean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper. Analytical Chemistry35(12), 1950-1953[Mango Drying Machine/Solar Fruit Drying Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Double-Wall Fabric PVC Inflatable Boat Fabric PVC Coated Drop Stitch FabricAuthor for correspondence