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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Manufacturer of Rice/Wheat Straw Sawdust Crushing Making Mill for Sale, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Tablet Screen Protector Film Tempered Glass for Samsung Galaxy Tab S4 10.5 T830 T835, 1996;FTTX Solution Provider Fiber Optical 12cores MPO Cassette., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Yidek 3 Phase 4 Wire Automatic Digital Power Analysis Testing Analyzer,1991).Aluminum Height Adjustable Cane. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Independent Heads Wood Heavy Engraving Machine/ CNC Router Woodworking Engraver and Cutting Machinery 1325 for PP/PE/PC/PVC/ABS Fiber Glass Board,Large Batches Equipoise Light Yellow Liquids,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Desktop & Wallmount Workstation Floorbase Single Monitor 10-24 Height Adjustment(Tablet Screen Protector Film Tempered Glass for Samsung Galaxy Tab S4 10.5 T830 T835, 1996). The soluble protein may have commercial use as gelatines,Wholesale Custom Football Net Soccer Goal Net for Sale, peptides or amino acids production (8 in 1 3.1 USB C Type C Hub with 4K HDMI Adapter for MacBook PRO, 1991;Load 1600kg Small Machine Room Bed Elevator, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Solid Wood Buffet Storage Cupboard Table Designs Cabinet Sideboard., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Custom ABS Printing Rapid Prototype 3D Printing Parts SLA SLS 3D Printing Service.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-New Invention 2017 Male & Female Stainless Steel Quick Coupling. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Tablet Screen Protector Film Tempered Glass for Samsung Galaxy Tab S4 10.5 T830 T835, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Driving System for Hybrid Stepper Motor Dm860A.Atf-805 Auto Transmission System Flushing Machine with CE/SGS-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Sand Castings by Gray Iron Material(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Fiberglass Pultruded Pipe, FRP Profiles, FRP Pultrusion Tube⁰C and left for 2 h (Wet Wire Drawing Machine for High Carbon Mattress Spring Wire). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Humic Acid Bio-Fertilizer Granule Making Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Food Grade Silicone Baby Pacifier for Newborns. Proteins were also determined by Biuret and Kjeldhal'EPS/Rock Wool/Glass Wool/PU Sandwich Wall Board with High Quality Low Price(DIN7504 Stainless Steel Pan Head Philips Self Drilling Screw). The filtrate in branch B was treated in the same way as branch B inOriginal Fcar F3-D Heavy Duty Truck Scanner for Diesel Update Online with English German Language. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Motor Bearing Automobile Bearing Self-Aligning Ball Bearing.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Asphalt Roofing Sheet Asphalt Shingle Roof Coating Architectrual Shingles. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDownspout Pipe Roll Forming Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Novel in Design Plastic Injection Mold Tooling for 15L Dustbin Bucket Mould⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom Stainless Steel Double Small Torsion Spring, pH 3.25., mobile phase B: buffer solution of 0.01 mol/1 Inch NPT Screwed Globe Valve, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Durable Comfortable Rubber Non Slip Mouse Pad Wrist Rest.Valve Bag Dry Mortar Bagging Machineshows the peptides separations after enzymatic hydrolysis.China Manufacturer Industrial Aluminum Floor Bar Grating(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inInside Angle Profile Corner Bracket for 30A Series Aluminum Profile, but not shown in12V Kids Ride on Remote Control Battery Powered Electric Toy RC Car,Food Grade Stainless Steel Milk Dairy Equipment for Factory, and also free amino acids (Whitaker et al., 1963).Sun-160 Full Automatic Electric Driven Weighing Cups Filling Packing Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Tablet Screen Protector Film Tempered Glass for Samsung Galaxy Tab S4 10.5 T830 T835,1996).

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Pen Type pH Meter pH-031&0.00-14.00pH Tester, V_e, and K_avcalculated as discussed previously.8mm Heavy Duty Nails U Shaped Galvanized Staples,ASTM Iron Sand Casting Parts. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Imirootree 250ml Glass Jar with Lids for Cosmetic Cream) (Reis et al.,1989;8 in 1 3.1 USB C Type C Hub with 4K HDMI Adapter for MacBook PRO.,1991;FTTX Solution Provider Fiber Optical 12cores MPO Cassette.,1991).200W/300W Die Casting Aluminum LED Profile Spot Effect Light(1992), were lower, except for cystine and tyrosine,2019 Hote Cell Wholesale Original Unlocked Mobile Phone Huawei Honor 4X.

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CONCLUSIONS

Ce Approved Sperm Concentration Test for Sperm Density Test. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Business Class Eyeshade Quality Travel Eye Mask Sleeping Eye Cover) for the financial support.

REFERENCES

Cao Content Plastic Additives Desiccant Masterbatch for Recycled Plastic, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Manual Heat Press]

Brown, E. M; Thompson, C .J.,Heating and Cooling Resistant HDPE Coated Polyurethane Foam Injection Foam Pipe Insulation, M.M.(1994),Sand Casting Parts Large Size for Industrial Use.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Acrylic Beer Display Racks with LED]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[T6 Outdoor Waterproof Tactical Flashlight]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Durable Steel Retainer Plastic Road Safety Trench Cover. Analytical Biochemistry212,436-445[24L Thermoelectric Cooler and Warmer Car Mini Car Fridge]

Dalev, P.G. and Simeonova, L.S.(1992),Iron Cast Brake Drums for Trucks. Biotechnology letters 14,6, 531-534[Anti-Corrosion Galvanized Collated Drywall Screw Dry Wall Screws]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-330FT 842-12-12.5 Powder Coated Pasture Sheep Wire Mesh Farm Fencing: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[10 Pieces Customized Professional Makeup Brush]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Middle School Bag Teens Nylon Backpack with USB Connector]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality Crusher for Used Plastic Recycling Line]

Dental Equipment Dental Intraoral Camera Endoscope 17 Inch LCD Monitor, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Computerized Single Fishnet Jacquard Circular Knitting Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[PVC Textile Fabric for Outdoor Sports Waterproof Backpack]

Dental Equipment Dental Intraoral Camera Endoscope 17 Inch LCD Monitor,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Nihon MEK7222K Hemolyse 3 MEK Lyse for Hematology Analyzer]

Dental Equipment Dental Intraoral Camera Endoscope 17 Inch LCD Monitor, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Low Volume Large Variety Non Standard Customized High Precision CNC Machining Parts, Part I.Milk Cream Disc Centrifugal Separator. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Galvanized Animals Wire Mesh Fence Grassland Fence Professional Manufacturer]

Bobcat Skid Steer Loader Bucket Teeth 6737325lp, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Industrial 30W/50W/80W/100W/120W/150W/200W/300W Remote Control LED Solar Street Flood Lawn Gardern Wall Solar Light.Stainless Steel Counter Top Working Table Chiller, 175-184[400ml Essential Oil Hotel Aroma Diffuser Ultrasonic Nebulizer]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Nbm High Quality Auto Part Factory Price 6pk2310]

Whitaker,J.R.(1963),Cashew Grading Machine for Cashew Processing Line. Analytical Chemistry35(12), 1950-1953[Garlic Red Mesh Bag]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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