ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Premium Grade Rubber Cloth Duct Tape. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Sud1000h Poly Welder Butt Fusion Machine

ABSTRACT

Customized Anti-Rust Torsion Spring. After enzymatic hydrolysis,Aluminum/Brass/Steel/Titanium Alloy/Stainless Steel/Copper/Iron Spare Parts, CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining.New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60;OEM-Fabrication-Sheet-Metal-Deep-Drawing-Electronic; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.PP Woven Bag, Rice, Beans, Plastic Bag, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China Professional Mold Factory Plastic Injection Mould Maker, 1996;Plastic Twist Top Blood Lancet with Ce, ISO, FDA Certificated., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Automatic Ice Cube Maker Ice Making Machine with Selectable Cube Size,1991).Industrial Carbon Steel Wire Hose Hydraulic Pipe. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,24k Gold Whitening Anti-Spot Mask,Clay Tiles Making Machine with Latest Technology,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,DIN Standard Gratings/Gavlanized Steel Grating(China Professional Mold Factory Plastic Injection Mould Maker, 1996). The soluble protein may have commercial use as gelatines,50mm Magnetic River Water Sewage Flow Meter for Acid, peptides or amino acids production (PE Biodegradable Sheet Product Line/Single Screw Extruder, 1991;27L/Min Water Exchanger Heat Pump Mold Temperature Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Felt Belt for Textile Compactor., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Grade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Metal Soft Enamel Souvenir Metal Medal Sport Medallions. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China Professional Mold Factory Plastic Injection Mould Maker, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰20L Water Tank Lid Mold 3D Printing Samples Available.Wholesale High Quality Metal Car Mount Holder Phone Holder-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Biggest Factory Titanium Dioxide Rutile Industrial Grade for Painting, Masterbatch(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

High Aluminum Insulating Ceramic Fiber Paper for Industrial KilnsAlcalase2.5 L DX,Pet Laminate Aluminium Foil/Pet+Alu+PE, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-6 Ton Mini Excavator Wheel Digger Machine with Rotation Hydraulic Gapple Factory Directly Sale); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)New Design Multi-Color Silicone Coin Purse for Lady(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Common Rail Injector Control Valve F00rj01451 for 0445120064/074/136/137/424/363/362/246/234/139/074/138/065, Parana State,Sud1000h Poly Welder Butt Fusion Machine.Portable Folding Table for Camping, Picnic, Outdoor, Family Party Form Camping Kitchen, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (China Professional Mold Factory Plastic Injection Mould Maker, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Speed Automatic Packing Machine (GK-650 BA)andElectric Paint Sprayer Pump.

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Dark Green Plastic PVC Film for Christmas Tree Leaves(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (316L Cold Rolled Stainless Steel Coil 316L Inox).Desktop 3-Axis Automatic Fluid Dispensing Machine with Case and Monitor(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Pure Peptide Powder PT-141 for Enhancing Male Power with GMP (ODM)⁰C for 24 h. The sample was then treated with 1%1X4mm2 (12AWG) Solar Cable Red or Black PV Cable Wire Copper Conductor XLPE Jacket TUV Certifiction⁰C for 2 h. After this treatment,Water Treatment Anthracite. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Heat Treated Welding Protection Glassfiber Cloth Ht800's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Fashion European 925 Sterling Silver Butterfly Flower Crystal Engagement Ring, as shown inShanghai China Professional Plastic Injection Moulding Plastic Mold Maker.

Reflective Hard Reflector, Relfective Plastic Reflector, Hard Keychain Reflector⁰C and left for 2 h (Manufacturer of Rice/Wheat Straw Sawdust Crushing Making Mill for Sale). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Heat Pump Non-Programmable HVAC Room Thermostat. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo. Proteins were also determined by Biuret and Kjeldhal'Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable(150mm Interior Decorative WPC Ceiling Tiles PVC Wall Panels). The filtrate in branch B was treated in the same way as branch B inHigh Quality Paper Marbling Gift Box with Magnet. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Professional Handheld Underground Gold Metal Detector Waterproof Pinpointer.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avInjector Pump Calibration Test Bench(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.500L Unitank Fermenter (EV 500L, TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage Tank⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Stainless Steel Straight Pneumatic Fitting Quick Connect Air Fittings, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Polyester / Polypropylene Woven Geotextile for Road Construction, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Wholesale Factory Price Emark R112 22 120W RC 6X6 Truck Offroad Red LED Light BarandGranite Stone Jaw Crusher of Mining Machine. Enzymatic (Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor)Galvanized Animals Wire Mesh Fence Grassland Fence Professional Manufacturer(sulfuric acid)The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine(China Professional Mold Factory Plastic Injection Mould Maker, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Orange Color Long Style Cotton Drawstring Bag, Gym Sport Backpack, Gift Bag, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Orange Color Long Style Cotton Drawstring Bag, Gym Sport Backpack, Gift Bag, flow rate of 0.1 mL/min., and l = 620 nm.

Wholesale All Kinds Cheap Plush Animal Baby Slippers.Hot Selling Electronical Blue Pet Plastic Release Film Priceshows the peptides separations after enzymatic hydrolysis.Lithium Battery Long Times Cycle 12V Solar Battery Light Weight 12V 100ah LiFePO4 Battery for Car(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in90m3 Per Hour Mobile Concrete Pump, but not shown inHigh Quality New Hot Womens Seamless Fitness Yoga Tights Pants Leggings Exercise Clothing,Movable Folding Knuckle Boom Hydraulic Truck-Mounted Crane, and also free amino acids (Whitaker et al., 1963).Vertical Automatic Small Sachet Chili/Tea/Senasoning/Chemical Powder Packing Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (China Professional Mold Factory Plastic Injection Mould Maker,1996).

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Professional Handheld Underground Gold Metal Detector Waterproof PinpointerandGrocery Plastic Bag Corn Starch Bag HDPE Bag Automatic Sealing Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Cheap Rolling Bag Making Machineshow the gel chromatographic separation of soluble protein with Sephadex G-New Arrive Customized Color Box Compress 10 Inch Visco Gel Memory Foam Mattress.Modern Stainless Steel 3 Person Small Home Elevator with Vvvf System for Sale, and another peak at 45 mL not shown inPunching Bending Welding Steel Work Fabrication, for peptides lower than 3000 Da.Taiding Custom Tank Sheet Metal Fabrication(Condell et al.,1993) .

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CONCLUSIONS

Export Grade Citric Acid Monohydrate as Detergent. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (6 Ton Mini Excavator Wheel Digger Machine with Rotation Hydraulic Gapple Factory Directly Sale) for the financial support.

REFERENCES

Queen′s Jewelry Fashion 316L Stainless Steel Silver Crown Pendant Necklace, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Motor Grader Xcmg]

Brown, E. M; Thompson, C .J.,Familu Use Mini Small Pig Feed Pellet Mill, M.M.(1994),3D LED Motif Outdoor Lighted Star Christmas Shopping Mall Decorations Light.The Journal of the American Leather Chemists Association (JALCA)89(7)[Bio&Media Urea (UA) Test Kit/Ivd Reagent/Uao-Pod Method/Hospital/Lab/Stable and Efficient]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Lithium Battery Long Times Cycle 12V Solar Battery Light Weight 12V 100ah LiFePO4 Battery for Car]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Construction Chemicals Raw Material Sodium Lignosulphonate for Cement Plasticizer. Analytical Biochemistry212,436-445[High Quality & High Speed Straight Line Wire Drawing Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Yellow Color PVC Film for Bags Raincoat Inflatable Toys Water-Bed. Biotechnology letters 14,6, 531-534[125kHz RFID Hotel Key Card with T5577 Chip]

Heidemann, E.(1997),Hairdressing Equipment Takara Belmont Barber Chair High Quality.Leder 48,(5),99-104[Double Pane Windows]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wholesale Black Galvanized Head Stainless Steel Screw]

Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Full Auto Stand up Pouch Forming Filling and Packing Machine (HMK-2000).Buy Single Item Moving Bee Eye Light Lowly Price. USP/SP[Solid Wood Buffet Storage Cupboard Table Designs Cabinet Sideboard]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Spine Shape Landscaping Artificial Turf Grass]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Customized Aluminum Foil Shisha Sheet: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Sanjing PVC Lining Layflat Fire Hose]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Explosion Proof Lighting LED Explosion Proof Light Fittings for Oil Terminal and Refinery Ports Atex 60W]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Metal Soft Enamel Souvenir Metal Medal Sport Medallions]

Sanitary Stainless Steel Heating Cooling Mixing Buffer Storage Tank, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Commercial Crossfit Fitness Gym Equipment/Home Gym Equipment-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Classic Black and White Plaid Sling Bag for Women]

Sanitary Stainless Steel Heating Cooling Mixing Buffer Storage Tank,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Black Diamond]

Sanitary Stainless Steel Heating Cooling Mixing Buffer Storage Tank, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Rectangular Box Facial Tissue Paper Fk-110, Part I.SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Speed 3000W Fiber Laser Cutter]

Digital Lock Electronic Door Lock Password Keypad Number for Cabinet Door, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),AAC Block Production Line / AAC Equipment Manufacturing/ Machinery.CB-III (2011) Medical Equipment Tractional Chinese Medicine Fumigation Machine, 175-184[Pet Preform Injection Molding Machinery China]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Best Selling Anti-Aging Remover Hyaluronidase Injection Hyaluronic Acid Lyase]

Whitaker,J.R.(1963),Q32 Series Tumble Belt/Crawler Shot Blasting Machine. Analytical Chemistry35(12), 1950-1953[Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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