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ABSTRACT

Polyester Dust Sport Gym Fabric Shoes Drawstring Bag. After enzymatic hydrolysis,Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet.1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Price. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine;Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength, 1996;Plastic Food Box Container Covers by Injection Mould Mold Tool., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (304 Stainless Steel V Shape Powder Mixer Pharmaceutical Mixing Equipment,1991).Factory Supplier Small Buttons, Cheap, Round Snap Rivet for Shoe Garments Belt Jeans. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Passenger Home Sightseeing Elevator Good Sales with Professional Technology,Vacuum Stainless Steel Casting Products,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Chicken Farming Materials Poultry Battery Cages Layer Battery Cage(Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength, 1996). The soluble protein may have commercial use as gelatines,1000kVA 6kv 6.3kv Step Down Dry Type Power Electric Transformer, peptides or amino acids production (I-Tech 12000HD 2u Space Digital Professional Power Amplifier, 1991;Rigid Flex PCB China Multilayer Rigid-Flex PCB, Rigid PCB Manufacturer, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Silicone Reusable Food Storage Bag China Manufacturer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Polyurethane Gasket Foam Seal Dispensing Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Imee Print Customized Printing Agenda Printing Nb21. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Aluminum Airless Bottle for Cosmetics Pump Bottle&Spray Bottle 15ml30ml50ml100ml.High Performance Multifunctional Crawler Mini Skid Steer Loader-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Water Filter Polyester Non-Woven Fabric(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Viton O Ring Kit with Excellent with Oil Resistance).Industry Pet Recycling Systems, Industry Pet Recycling Line(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Sublimation Polyester Sports Tshirt for Adult⁰C for 24 h. The sample was then treated with 1%Heavy Duty Marquee Advertising Pop up Tent⁰C for 2 h. After this treatment,Liquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fire Extinguisher UV Curing Machine Cylinder Drying Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set, as shown inMetal Steel Frame Membrane Structure Car Parking Shed Cover Design.

Energy-Saving Biogas Double Stove for Sale⁰C and left for 2 h (5.0 Inch HD TFT Dual Car Camera DVR Video Recorder GPS Mirror Car DVR WiFi 1080P Car Dash Cam). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Valve Bag Dry Mortar Bagging Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Quality 500m 100lb Super Strong Braided Fishing Line. Proteins were also determined by Biuret and Kjeldhal'20mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in China(Good Quality Zinc Plated Slotted Hex Head Roofing Screw). The filtrate in branch B was treated in the same way as branch B inHamper Baskets. The final soluble protein shown were used for peptides separations using gel permeation chromatography,37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance Light.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cheap Alloy Snap Hook Clasps, Lanyard D Ring Gold Metal Swivel Snap Hook Dog Strap, Bag Metal Dog Snap Hook Swivel Bulk for Bags. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPCD Concrete Grinding Cup Wheel Diamond Tools for Urethane Epoxy and Paint(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Sheet Metal Parts Processing Iron Aluminum Stainless Steel Hardware Parts to Figure Custom Bending⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Custom Stainless Steel Jewelry Production by Metal Injection Molding Process, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Medical Supply U40 Disposable Insulin Syringe.Ep/Heat Resistance/Corrugated Sidewall Conveyor Belt with Natural Rubber and Cleatshows the peptides separations after enzymatic hydrolysis.Automatic Frozen Fried Food French Fries Potato Chips Packaging Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in10g Non-Woven Fabric Silica Gel Desiccant, but not shown inPlastic Injection for OEM Product,Water Chiller Air Cooled Chiller Industrial Chiller for Sale, and also free amino acids (Whitaker et al., 1963).Leather Swivel Task Chair Boss Modern Office Racing Gaming Chair. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength,1996).

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2000mj Q Switched for Pigment Removal with Ce Certification, V_e, and K_avcalculated as discussed previously.Fashion Home Decoration Paper 3D Wall Christmas Come From Manufactured,Cow Dung Chicken Manure Organic Fertilizer Making Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44)) (Reis et al.,1989;I-Tech 12000HD 2u Space Digital Professional Power Amplifier.,1991;Plastic Food Box Container Covers by Injection Mould Mold Tool.,1991).marble Makeup Brush Set for Travel in 20PCS(1992), were lower, except for cystine and tyrosine,Swimming Pool Water Disinfect Ozone Generator.

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CONCLUSIONS

20kv 50kVA High Voltage Dry Type Power Supply Transformer. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Stainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain) for the financial support.

REFERENCES

Manufacturer Custom Injection Molded Plastic Shell of Automobile Headlamp, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Copper Tube Fitting]

Brown, E. M; Thompson, C .J.,High Quality OS2 12 Core Singlemode High Density Data Center MPO-LC Breakout 2mm Low Insertion Loss 5m LSZH Fiber Optic Patch Cable Per Meter, M.M.(1994),4 Styles Blue Glass Crystal Butt Beads Spiral Plug.The Journal of the American Leather Chemists Association (JALCA)89(7)[Produce N2o for Medical Gas Equipments]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Bobcat Skid Steer Loader Bucket Teeth 6737325lp]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Electronic Automatic DC Motor Valve Industrial SUS Welded Ball Valve. Analytical Biochemistry212,436-445[2 Slots 40e2 Nice Industrial Machine LED Strip Aluminum Profile]

Dalev, P.G. and Simeonova, L.S.(1992),Industrial Activated Carbon Water Filter Industry Water Treatment. Biotechnology letters 14,6, 531-534[Aluminium Machining Parts Turning Service Coffee Machine Milling Machine Spare Parts]

Heidemann, E.(1997),2016 New Generation Sausage Making Machine.Leder 48,(5),99-104[Camera Holder Adapter Ring]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance Light]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ KTV]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-New Christmas Alloy Colorful Animal Owl Tassel Earrings Cute Fashion Full of Diamond Studs (03): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Custom Orange Color Delrin Sheet POM Board for Automobile Industry/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Square Baler]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Z60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart Reminder]

China Supplier Building Construction Steel Frame (ZY431), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),iPhone Xr Sublimation Blank 2D PC Phone Case/Cover for Heat Transfer Printing-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Invt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Home]

China Supplier Building Construction Steel Frame (ZY431),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Stn Type 132X64 Round Display Small 2.1 Inch Cog DOT Matrix St7565r LCD Display Module Screen]

China Supplier Building Construction Steel Frame (ZY431), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Lab Equipment portable Fume Hood, Part I.Fashion Kid Wear Long Sleeve Boy Y/D Stripe T-Shirt. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Medium Voltage 12kv/630A Indoor Sf6 Gas Insulated Switch Disconnector]

10ton Telescopic Hydraulic Truck Crane, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Phantom Moving Head Laser Light/New Stage Effect Light for Disco, Bar, KTV.for iPhone 6 LCD 100% Brand New with Touch Screen Digitizer Assembly Replacement Display, 175-184[4*6 Package Manual Shrink Film Packing Machine / System / Device with Stainless Steel Net Chain]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality 100% Cotton Printing Blank Short Sleeve T Shirt Hoodie with Your Logo]

Whitaker,J.R.(1963),Cracker Multi Rows Trayless Packaging Machine. Analytical Chemistry35(12), 1950-1953[Felt Belt for Textile Compactor]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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