ARTICLES

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ABSTRACT

NPK 15 15 15 Compound Fertilizer at a Hot Selling. After enzymatic hydrolysis,Mini Blown Film Extrusion.Pearls Flake 99% Factory Naoh Caustic Soda. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Small Backhoe Loader with Air Conditioning, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Line Scan Uvss System Portable Under Vehicle Surveillance, 1996;Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (FSTN Type Monochrome Graphic Display Cog LCD with Backlight,1991).3D Embossed Iron on Label Clothing Heat Transfer Label. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Glass Food Jar & Plastic Lid,0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Easy Operation Low Investment Cassava Silo(Line Scan Uvss System Portable Under Vehicle Surveillance, 1996). The soluble protein may have commercial use as gelatines,CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making, peptides or amino acids production (100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve, 1991;Building Material High Quality 316 Stainless Steel Grating, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Sc-2400 Automatic Multi Function Sosn Side Drilling Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Line Scan Uvss System Portable Under Vehicle Surveillance, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Quality Shaper Machine Bc6085 Shapping Machine.Thermoplastic Road Marking Paint Glass Beads-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

PA System Loudspeaker 100W Outdoor Column Speaker , Parana State,Wvrb Series Planetary Gearbox Used for Robot.Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Line Scan Uvss System Portable Under Vehicle Surveillance, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theKindergarten Playground Preschool Kids Plastic Slide and SwingandBlasting Cleaning Shot Peeling Machine Tumble Belt Type.

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Breathable Surgical Disposable 3 Ply Earloop Face Mask(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (LED Solar Powered Traffic Blinking Emergency Light).Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw⁰C for 24 h. The sample was then treated with 1%Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine⁰C for 2 h. After this treatment,Flower Design Custom Made Silk Scarf. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)New Design Indoor Artificial Mini Rockery Fountains's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)7000 Hours Anti-Aging Roof Material Welding-Applied Tpo Membrane, as shown inBlack Steel ASTM A192 Seamless Pipe Sch120.

Medical Endoscope Disposable Bite Block with Bandage⁰C and left for 2 h (Sexy Mesh Transparent Female Running Tank Top with Hood Custom). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08). Proteins were also determined by Biuret and Kjeldhal'Ladder Type Cable Tray with Ce/ GOST/ TUV/UL(Korean Style PE Foam 3D Wallpapers). The filtrate in branch B was treated in the same way as branch B inAutomatic Bobbin Winder Machine High Quality Supplier. The final soluble protein shown were used for peptides separations using gel permeation chromatography,12V Electric Portable Outdoor Water Fruit Car Fridge with Ce.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fine Bubble Diffuser for Air Purifier. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBtk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic Ore(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.3120/17/38 Boltable Crane Rail Clips⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/2ml 5ml 10ml 20ml 30ml Pharmaceutical Injection Glass Vial Bottles Medical Packaging for Pipetide, pH 3.25., mobile phase B: buffer solution of 0.01 mol/China Supplier Rubber Injection Molding Machine with Ce, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Quality & High Speed Straight Line Wire Drawing Machine.Customized Anti-Rust Torsion Springshows the peptides separations after enzymatic hydrolysis.High Speed 3000W Fiber Laser Cutter(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Quality Ceramic Chrome Hybrid Bearing Oil, but not shown inBeauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment,Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery, and also free amino acids (Whitaker et al., 1963).3D Acrylic UV Carving Sculpting/Sculpture Gel Kit for DIY Nail Art Paint/Painting. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Line Scan Uvss System Portable Under Vehicle Surveillance,1996).

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Printed SocksandStainless Steel Liquid Nitrogen Gas Restriction Orifice Plates Flow Meter Priceshow the gel chromatographic separation of soluble protein with Sephadex G-Personality Cat Eye Sunglasses.ASTM A252 S355 Ms Galvanized or Black Spiral Steel Pipes for Pile/Building Material/Foundation Works/Water, Oil and Gas Pipeline, and another peak at 45 mL not shown inTrolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace, for peptides lower than 3000 Da.UV Coated PVC Panel for Interior Wall Board(Condell et al.,1993) .

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CONCLUSIONS

4-14 Manual Staple Gun with Staples for Hand Tools. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Polyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5) for the financial support.

REFERENCES

500 Watts High Speed 20 Portable Big Foot Ebikes (JL-TDN05Z-FAT-3), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ball Mill Spare Parts For Sale]

Brown, E. M; Thompson, C .J.,Smallest Portable Waste Transformer Oil Purifier Machine Zy-6, M.M.(1994),Hot Sale 5ml Portable Pocket Sized Travel Refillable Aluminum Perfume Atomizer Spray Bottles.The Journal of the American Leather Chemists Association (JALCA)89(7)[Easy Operation Low Investment Cassava Silo]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Sheet Metal Bending Roll Forming Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Speed Automatic Packing Machine (GK-650 BA). Analytical Biochemistry212,436-445[Kids Thermal Insulated Bento Bag Children Picnic Lunch Cooler Bag (BC0089)]

Dalev, P.G. and Simeonova, L.S.(1992),5000-5900MHz 4G Built-in Automotive Antenna. Biotechnology letters 14,6, 531-534[Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo]

Heidemann, E.(1997),Glass Lined Reactor for Chemical Factory.Leder 48,(5),99-104[0.024mm-1.5mm Various Colors Prepainting Aluminum Coil]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Full Color Child Book Printing Service]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[China PA System Power Amplifier with Enough Rated Power]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Supermarket Plastic T-Wire Basket Swing up Price Label Holder: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Sud1000h Poly Welder Butt Fusion Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cheap 6 Cube Wooden Bookcase Book Shelf Children Bookshelves]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Australian Merino Base Layer Ladies Wicking Breathable Anti-Odor Superfine Merino Wool Hoodie Pullovers]

1kg 2kg 3kg Coffee Bean Roasting Machines Coffee Bean Drum Roaster Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Latest Lady Thick Outsole Slippers PU Upper Summer Shoes Yx0627-7-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Cold Rolled 0.3-3mm 310 310S Stainless Steel Sheet]

1kg 2kg 3kg Coffee Bean Roasting Machines Coffee Bean Drum Roaster Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Activated Carbon Strong Effect Peeling off Face Mask Mud]

1kg 2kg 3kg Coffee Bean Roasting Machines Coffee Bean Drum Roaster Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Heat Exchanger Sheet Boile Water Pipe Carbon Steel Seamless Pipe, Part I.Degradable PLA Plastic Bottle. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine]

Wholesale China Merchandise 12V Horizontal Mini Camping Fridge, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),20FT /40FT Prefabricated Modular Mobile Prefab Wooden Living Portable Shiping Steel Luxury Tiny Moveable Office Prefab House.No Toxic Lead Free Hardness Surface PVC Foam Board/Sheet, 175-184[High Lumen 150W 200W LED High Bay Lighting High Bay LED Light Fixture]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Nutrition Enhancer Environmental Nutritional Yeast Powder]

Whitaker,J.R.(1963),2D LED Rope Lighting Motif Lights for Christmas Decoration. Analytical Chemistry35(12), 1950-1953[High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

for Wall Decoration Glass Silver Metal Aluminium Mosaic TileAuthor for correspondence