ARTICLES

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ABSTRACT

Plastic Colored Custom Foam Shampoo Pet Bottle with Cosmetic Pump. After enzymatic hydrolysis,Low Frequency 3000W 24V 230V Inverter with Battery Charger.Amazon Best Selling Sports Ankle Support Sleeves Compression. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Pen Type pH Meter pH-031&0.00-14.00pH Tester;8mm Heavy Duty Nails U Shaped Galvanized Staples; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.ASTM Iron Sand Casting Parts, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (200W/300W Die Casting Aluminum LED Profile Spot Effect Light, 1996;2019 Hote Cell Wholesale Original Unlocked Mobile Phone Huawei Honor 4X., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Ce Approved Sperm Concentration Test for Sperm Density Test,1991).Cao Content Plastic Additives Desiccant Masterbatch for Recycled Plastic. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Heating and Cooling Resistant HDPE Coated Polyurethane Foam Injection Foam Pipe Insulation,Sand Casting Parts Large Size for Industrial Use,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Durable Steel Retainer Plastic Road Safety Trench Cover(200W/300W Die Casting Aluminum LED Profile Spot Effect Light, 1996). The soluble protein may have commercial use as gelatines,Iron Cast Brake Drums for Trucks, peptides or amino acids production (Eco-Friendly Barware Food Grade Stainless Steel 304 Reusable Custom Straws, 1991;Folding Aluminium Easy Move Choral Stage with Wheels for School, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Factory Direct Sale Turnbuckle Tensioner Open Body Orchid Tightener Rigging Screw Quantity Discounts., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.330FT 842-12-12.5 Powder Coated Pasture Sheep Wire Mesh Farm Fencing.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Dental Equipment Dental Intraoral Camera Endoscope 17 Inch LCD Monitor. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (200W/300W Die Casting Aluminum LED Profile Spot Effect Light, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Computerized Single Fishnet Jacquard Circular Knitting Machine.Low Volume Large Variety Non Standard Customized High Precision CNC Machining Parts-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Milk Cream Disc Centrifugal Separator(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

4X4 Wd Car Tail Light for Ranger 2012-2016, Parana State,Sun-160 Full Automatic Electric Driven Weighing Cups Filling Packing Machine.Multicore Underground Aluminum Conductor Steel Tape Armored Power Cable, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (200W/300W Die Casting Aluminum LED Profile Spot Effect Light, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theDIN934 Hex Nut/304 Stainless Steel Hexagon Nut with DIN933 Hex Bolt/Stainless Steel Bolt and NutandStarch Doy Bag Packaging Machine.

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New Design Exaggerated Cute Gem Cotton Tassel Brand Earrings(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration).Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hot Sale Power Press Perforated Metal Punch Machine⁰C for 24 h. The sample was then treated with 1%100%Polyester Satin Fabric for Blouse⁰C for 2 h. After this treatment,Shenzhen Manufacturer High Quality Metal CNC Precision Machining Titanium/Stainless Steel/Brass Custom Aluminum Case/Box/Casing/Enclosure. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Tungsten Carbide Extension Adapter Boring Bars for External Turning Tools's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High-End Compression Rash Guards, Long Sleeve Compression Shirt, as shown inSoldering Machine LED Module PCB Hot Air Soldering Station.

High Quality 100% Cotton Printing Blank Short Sleeve T Shirt Hoodie with Your Logo⁰C and left for 2 h (Ground Finish Molybdenum Tzm Rod). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Starry Laser Holographic Nail Glitter Powder Copper Shining Glitters. Proteins were also determined by Biuret and Kjeldhal'9~12 Inch Mechanical Backing Plate( 20%Sc). The filtrate in branch B was treated in the same way as branch B inCommon Rail Auto Parts Denso Valve for 095000-6790 Injector. The final soluble protein shown were used for peptides separations using gel permeation chromatography,DIN929 Low-Cost Supply Hexagon Weld Nut.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Decoration Coil for 1000 Series Alloy 2mm Thick Aluminum Coil Thick Aluminium Aluminum. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Efficiency Solar Cell Photovoltaic 300W Hot Sell(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.SGS Steel Lab Acid and Alkali Resistance Fume Hood Laboratory Furniture with Ce Certification (JH-FC019)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Super Fireproof Antibiosis Household Spc Vinyl Floor Plank, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Feet Protection Industrial Cow Split Leather Working Safety Shoes, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Manufacturer of Soda Ash Dense 99 2% PriceandSoldering Machine LED Module PCB Hot Air Soldering Station. Enzymatic (220V/380V/400V/415V/460V/525V Material Handling Equipment Heavy Duty 50 Ton Electrical Chain Hoist)Aluminum Type-C 3.1 Adapter with 4K HDMI/VGA/SD/TF/RJ45/USB 3.0/Pd 100W(sulfuric acid)High Safety Double Wire Security Metal Welded Wire Mesh Fence(200W/300W Die Casting Aluminum LED Profile Spot Effect Light, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Pneumatic Air Driven Liquid Booster Pump, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Pneumatic Air Driven Liquid Booster Pump, flow rate of 0.1 mL/min., and l = 620 nm.

Heavy Duty Wood Door Calibrating Wide Belt Sander.Barbed Wire Right off The Border Wall to Protect Homesshows the peptides separations after enzymatic hydrolysis.Magnifying Mirror Shaving Mirror 2X, 3X, 5X, 8X, 10X(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inNew Design 4*2 Street Cleaning Machine Sweeping Truck with Brushes, but not shown inMagnifying Mirror Shaving Mirror 2X,Aluminium 6061 Electronickelling Laser Engraved CNC Machined Parts UK, and also free amino acids (Whitaker et al., 1963).X-Humate 95% Min Super Sodium Humate (Nut Moradant). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (200W/300W Die Casting Aluminum LED Profile Spot Effect Light,1996).

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CONCLUSIONS

Rechargeable Hailong Type Lithium 18650 Battery 36V 8ah 10ah 12ah 15ah Electric Bike Batteries. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Stainless Steel Counter Top Working Table Chiller) for the financial support.

REFERENCES

Custom Velvet Gift Jewelry Packaging Pouch with Plastic Lock Close, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Residential Dumbwaiter]

Brown, E. M; Thompson, C .J.,Medical Packing Bags, Sterile Pouches, Medical Pouches for Sterilization, M.M.(1994),Galvanized Hexagonal Wire Mesh Gabion / Gabion Box.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hot Sale Instax Mini Camera Korea Style Photo Album]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Sterile Dsiposable Syringe for Dental 12ml]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-10X20FT Outdoor Folding Pop up Marquee Canopy Gazebo Tent for Sale. Analytical Biochemistry212,436-445[Wear Resistant Rubber Ceramic Wear Linier Plate for Chute Liner]

Dalev, P.G. and Simeonova, L.S.(1992),Must 4kw 2HP Pump Power Inverter Ags Bts Available. Biotechnology letters 14,6, 531-534[Industrial Rubber Nylon Conveyor Belting Automotive Parts]

Heidemann, E.(1997),Hot Sale Small Steel Silo for Poultry Feed.Leder 48,(5),99-104[Big Desk Lamp Shade Suppliers for Sale]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Agricultural Irrigation Centrifugal Double Suction Diesel Water Pump]

China Manufacturer Industrial Aluminum Floor Bar Grating, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Good Price Nasal Paste Packaging Machine.Powerful Full Hydraulic Safework Certificates Hgy13 15 17m Mobile Concrete Placing Booms. USP/SP[PP Woven Bag]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ASTM A240 321 Stainless Steel Sheet Price Per Kg]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Hardness Industrial Aluminum Extrusion Alloy Profile for Table Frame and LED Light Frame: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ CNC Lathe Machine Milling]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cheap New Design Light Prefab Steel Structure Chicken House Poultry Farm Building for Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Bending Sheet Metal Custom-Made Aluminum Bracket]

Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Reverse Rolled Wedge Wire Screen/ Jonson Screen Plate-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Pneumatic Air Driven Liquid Booster Pump]

Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Engine Parts Rubber High Quality Timing Belt 111ru25]

Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Ce Apporved New Model Aluminum 10W/15W/20W/25W/30W COB LED Downlight, Part I.Barbed Wire Right off The Border Wall to Protect Homes. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[White HPL 600*600mm Coffee Table]

Big Discount Mosquito Protection Window Screen Anti Mosquito Net Insect Screen, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),OEM Factory/Chrome Steel/ISO Certificate/Used in Machine/Pillow Block Bearings/Bearings/Bearing (UCP).Ms Gi Pipe Dn15~Dn400 Gi Pipe List, 175-184[Agricultural Machine Parts Crawler Rubber Track]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Full Automatic Top Quality Hot Sale Pine Chips / Wheat Straw Gasifier Power Plant]

Whitaker,J.R.(1963),Low-E Insulating Glass Curtain Wall. Analytical Chemistry35(12), 1950-1953[Air Cooled Scroll Type Water Chiller for Industrial Process]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Mini Blown Film ExtrusionAuthor for correspondence