ARTICLES

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ABSTRACT

Top China Forklift Supplier & Manufacturer. After enzymatic hydrolysis,Blue White Yellow Red Black Nylon Mosquito  Window Netting.Hot Sale Spraying Drying Equipment. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Food Kitchen Mini Milling Electric Grinding Maize and Flour Making Machine;LED Solar Powered Traffic Blinking Emergency Light; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Die-Casting LED Projector Light 840W Outdoor Sport Field Light High Mast Light 150lm/W, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Fiberglass Pultruded Pipe, FRP Profiles, FRP Pultrusion Tube, 1996;Humic Acid Bio-Fertilizer Granule Making Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Food Grade Silicone Baby Pacifier for Newborns,1991).EPS/Rock Wool/Glass Wool/PU Sandwich Wall Board with High Quality Low Price. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Motor Bearing Automobile Bearing Self-Aligning Ball Bearing,Asphalt Roofing Sheet Asphalt Shingle Roof Coating Architectrual Shingles,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Downspout Pipe Roll Forming Machine(Fiberglass Pultruded Pipe, FRP Profiles, FRP Pultrusion Tube, 1996). The soluble protein may have commercial use as gelatines,4mm Vinyl Plank Floor Lvt Click Lock Wood PVC Spc Click Vinyl Flooring, peptides or amino acids production (Novel in Design Plastic Injection Mold Tooling for 15L Dustbin Bucket Mould, 1991;Custom Stainless Steel Double Small Torsion Spring, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;1 Inch NPT Screwed Globe Valve., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Industrial Rubber Nylon Conveyor Belting Automotive Parts.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Aluminium Insert MDF Factory-Slotted Groove MDF in 12mm 15mm 18mm. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Fiberglass Pultruded Pipe, FRP Profiles, FRP Pultrusion Tube, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Small Leather Shoes Craft Laser Cutting Machine.Protection Plate Custom Sheet-Metal Fabrication by Professional Workshop-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Baby Toothbrush Kids Silicone Finger Brush, Baby Finger Toothbrush(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Plastic Colored Custom Foam Shampoo Pet Bottle with Cosmetic Pump, Parana State,2019 Wholesale New Notebook Computers for DELL Inspiron 17.3 Core I7-8550 16GB RAM 2tb HDD Radeon 530 DVD Win10 Laptop.Low Frequency 3000W 24V 230V Inverter with Battery Charger, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Fiberglass Pultruded Pipe, FRP Profiles, FRP Pultrusion Tube, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the20FT /40FT Prefabricated Modular Mobile Prefab Wooden Living Portable Shiping Steel Luxury Tiny Moveable Office Prefab Houseand Family Party Form Camping Kitchen.

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Amazon Best Selling Sports Ankle Support Sleeves Compression(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Galvanized Chain Link Wire Mesh/Diamond Wire Mesh/Chain Link Fence).Pen Type pH Meter pH-031&0.00-14.00pH Tester(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%8mm Heavy Duty Nails U Shaped Galvanized Staples⁰C for 24 h. The sample was then treated with 1%ASTM Iron Sand Casting Parts⁰C for 2 h. After this treatment,200W/300W Die Casting Aluminum LED Profile Spot Effect Light. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)2019 Hote Cell Wholesale Original Unlocked Mobile Phone Huawei Honor 4X's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Ce Approved Sperm Concentration Test for Sperm Density Test, as shown inMultifunctional Stainless Steel Mesh Filter Cylinder Punched Round Hole Shape Filter Tube.

Cao Content Plastic Additives Desiccant Masterbatch for Recycled Plastic⁰C and left for 2 h (Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Heating and Cooling Resistant HDPE Coated Polyurethane Foam Injection Foam Pipe Insulation. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Sand Casting Parts Large Size for Industrial Use. Proteins were also determined by Biuret and Kjeldhal'Durable Steel Retainer Plastic Road Safety Trench Cover( Used Cardboard Corrugator Line). The filtrate in branch B was treated in the same way as branch B inCe / ISO9001 / 7 Patents Approved Cable Buncher High Speed Stranding Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Iron Cast Brake Drums for Trucks.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Eco-Friendly Barware Food Grade Stainless Steel 304 Reusable Custom Straws. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFolding Aluminium Easy Move Choral Stage with Wheels for School(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.330FT 842-12-12.5 Powder Coated Pasture Sheep Wire Mesh Farm Fencing⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Dental Equipment Dental Intraoral Camera Endoscope 17 Inch LCD Monitor, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Computerized Single Fishnet Jacquard Circular Knitting Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable ChairandLow Volume Large Variety Non Standard Customized High Precision CNC Machining Parts. Enzymatic (Milk Cream Disc Centrifugal Separator)Bobcat Skid Steer Loader Bucket Teeth 6737325lp(sulfuric acid)Industrial 30W/50W/80W/100W/120W/150W/200W/300W Remote Control LED Solar Street Flood Lawn Gardern Wall Solar Light(Fiberglass Pultruded Pipe, FRP Profiles, FRP Pultrusion Tube, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Stainless Steel Counter Top Working Table Chiller, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Stainless Steel Counter Top Working Table Chiller, flow rate of 0.1 mL/min., and l = 620 nm.

Cashew Grading Machine for Cashew Processing Line.Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Setshows the peptides separations after enzymatic hydrolysis.4X4 Wd Car Tail Light for Ranger 2012-2016(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCow Dung Chicken Manure Organic Fertilizer Making Machine, but not shown in4X4 Wd Car Tail Light for Ranger 2012-2016,Multicore Underground Aluminum Conductor Steel Tape Armored Power Cable, and also free amino acids (Whitaker et al., 1963).New Design Exaggerated Cute Gem Cotton Tassel Brand Earrings. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Fiberglass Pultruded Pipe, FRP Profiles, FRP Pultrusion Tube,1996).

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Shenzhen Manufacturer High Quality Metal CNC Precision Machining Titanium/Stainless Steel/Brass Custom Aluminum Case/Box/Casing/Enclosure, V_e, and K_avcalculated as discussed previously.Tungsten Carbide Extension Adapter Boring Bars for External Turning Tools,High-End Compression Rash Guards, Long Sleeve Compression Shirt. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office) (Reis et al.,1989;Novel in Design Plastic Injection Mold Tooling for 15L Dustbin Bucket Mould.,1991;Humic Acid Bio-Fertilizer Granule Making Machine.,1991).High Quality 100% Cotton Printing Blank Short Sleeve T Shirt Hoodie with Your Logo(1992), were lower, except for cystine and tyrosine,Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office.

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CONCLUSIONS

Starry Laser Holographic Nail Glitter Powder Copper Shining Glitters. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Food Grade Stainless Steel Milk Dairy Equipment for Factory) for the financial support.

REFERENCES

9~12 Inch Mechanical Backing Plate, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Gate Valve Dn100]

Brown, E. M; Thompson, C .J.,DIN929 Low-Cost Supply Hexagon Weld Nut, M.M.(1994),Decoration Coil for 1000 Series Alloy 2mm Thick Aluminum Coil Thick Aluminium Aluminum.The Journal of the American Leather Chemists Association (JALCA)89(7)[Water Chiller Air Cooled Chiller Industrial Chiller for Sale]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Custom Logo Man Quartz Watch Fashion Wrist Watches for Men (WY-17016D)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Efficiency Solar Cell Photovoltaic 300W Hot Sell. Analytical Biochemistry212,436-445[13.56 MHz Fudan FM11RF08 Chip Card]

Dalev, P.G. and Simeonova, L.S.(1992),60m3/H Lamella Clarifier for River Water Treatment. Biotechnology letters 14,6, 531-534[Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken]

Heidemann, E.(1997),SGS Steel Lab Acid and Alkali Resistance Fume Hood Laboratory Furniture with Ce Certification (JH-FC019).Leder 48,(5),99-104[Helical Shaft Mounted Gearbox Nmrv Series Gearbox Right Angle Cast Iron Worm Gearbox]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Colombia Outdoor P5 Pantallas LED Display]

Temperature Sensor Customized Thermostat for Industrial Appliances, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Super Fireproof Antibiosis Household Spc Vinyl Floor Plank.High Quality Feet Protection Industrial Cow Split Leather Working Safety Shoes. USP/SP[Food Package Aluminum Foil Tray Making Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[5W Ultra-Thin Tiltable LED Down Light]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Soldering Machine LED Module PCB Hot Air Soldering Station: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Electrophoresis Assembly Infilltration Tank for Effluent Treatment]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Osyea-SL Ce Certified Gel Battery Buried LED Solar Street Light]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[PA System Loudspeaker 100W Outdoor Column Speaker ]

220V/380V/400V/415V/460V/525V Material Handling Equipment Heavy Duty 50 Ton Electrical Chain Hoist, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Aluminum Type-C 3.1 Adapter with 4K HDMI/VGA/SD/TF/RJ45/USB 3.0/Pd 100W-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Bsp Standard Malleable Iron Pipe Fitting Hexagon Nipple]

220V/380V/400V/415V/460V/525V Material Handling Equipment Heavy Duty 50 Ton Electrical Chain Hoist,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Soosan Excavator Hydraulic Hammer for Construction Demolition]

220V/380V/400V/415V/460V/525V Material Handling Equipment Heavy Duty 50 Ton Electrical Chain Hoist, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Safety Double Wire Security Metal Welded Wire Mesh Fence, Part I.Pneumatic Air Driven Liquid Booster Pump. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[130HP 4WD Large Farm Power Engine Tractor From High Quality Factory]

Heavy Duty Wood Door Calibrating Wide Belt Sander, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Magnifying Mirror Shaving Mirror 2X, 3X, 5X, 8X, 10X.Aluminium 6061 Electronickelling Laser Engraved CNC Machined Parts UK, 175-184[m.qj-ec.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Viton O Ring Kit with Excellent with Oil Resistance]

Whitaker,J.R.(1963),X-Humate 95% Min Super Sodium Humate (Nut Moradant). Analytical Chemistry35(12), 1950-1953[Metal Steel Frame Membrane Structure Car Parking Shed Cover Design]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

5.0 Inch HD TFT Dual Car Camera DVR Video Recorder GPS Mirror Car DVR WiFi 1080P Car Dash CamAuthor for correspondence