ARTICLES

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ABSTRACT

Shima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Condition. After enzymatic hydrolysis,Double Sides PVC Coated Fabric for Rafting Boat and Inflatable Boat.River Water Cleaning Sludge Sediment Machine Lamella Clarifier. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Polyester Coated Stainless Steel Ladder Type Metal Ties;2019 Ebay Hot Prefilled Salt Nicotine Tomato Sauce Disposable Vape Device; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.SDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt Welder, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Copper Laminated Steel Strip T2/C11000, 1996;Halal Empty Gelatin Capsule for Medicine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Valve Rubberwasher, Wholesale Hot Sale Rubber Silicone Seal Diaphragm,1991).Gym Fitness Exercise Sporting Body Building Bicep Press. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Chinese Forklift Hydraulic Manual Fd30 3 Ton Forklift Truck,Metal Steel Frame Membrane Structure Car Parking Shed Cover Design,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size(Copper Laminated Steel Strip T2/C11000, 1996). The soluble protein may have commercial use as gelatines,300X800mm 3D Ink-Jet Ceramic Interior Bathroom Wall Tile, peptides or amino acids production (Integrated LED Solar Street Sensor Light with Sensor Control for Highway 10W/20W/30W/40W/50W/60W, 1991;Polyester Dust Sport Gym Fabric Shoes Drawstring Bag, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Price.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Copper Laminated Steel Strip T2/C11000, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver.Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Vacuum Stainless Steel Casting Products, Parana State,Natural Jute Lace Burlap Ribbon for Bow Ribbon DIY Decoration.Chicken Farming Materials Poultry Battery Cages Layer Battery Cage, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Copper Laminated Steel Strip T2/C11000, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theGood Price Nasal Paste Packaging MachineandWear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office.

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1000kVA 6kv 6.3kv Step Down Dry Type Power Electric Transformer(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Custom Sheet Metal Stamping Bending Product Manufacturing Service).I-Tech 12000HD 2u Space Digital Professional Power Amplifier(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Rigid Flex PCB China Multilayer Rigid-Flex PCB, Rigid PCB Manufacturer⁰C for 24 h. The sample was then treated with 1%High Quality Silicone Reusable Food Storage Bag China Manufacturer⁰C for 2 h. After this treatment,Polyurethane Gasket Foam Seal Dispensing Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Imee Print Customized Printing Agenda Printing Nb21's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Aluminum Airless Bottle for Cosmetics Pump Bottle&Spray Bottle 15ml30ml50ml100ml, as shown inWater Chiller Air Cooled Chiller Industrial Chiller for Sale.

High Performance Multifunctional Crawler Mini Skid Steer Loader⁰C and left for 2 h (Custom Logo Man Quartz Watch Fashion Wrist Watches for Men (WY-17016D)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Chunke Portable UV Water Sterilizer for Water Purifier 150t. Proteins were also determined by Biuret and Kjeldhal'Long Sleeve Lace Beaded Muslim Bridal Gown Wedding Dress(13.56 MHz Fudan FM11RF08 Chip Card). The filtrate in branch B was treated in the same way as branch B inDeamino-Cys(1)-Harg-Gly-Asp-Trp-Pro-Cys(1)-Nh2. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Stainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Xjm Series Rubber Extruding Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avStop Dog From Jumping or Climbing Your Chain Link Fence.(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Water Filter Polyester Non-Woven Fabric⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Industry Pet Recycling Systems, Industry Pet Recycling Line, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Sublimation Polyester Sports Tshirt for Adult, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Automatic Moder Double Seal Nipple Drinking System for Broiler ChickenandHeavy Duty Marquee Advertising Pop up Tent. Enzymatic (Liquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer)Fire Extinguisher UV Curing Machine Cylinder Drying Machine(sulfuric acid)Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set(Copper Laminated Steel Strip T2/C11000, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Energy-Saving Biogas Double Stove for Sale, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Energy-Saving Biogas Double Stove for Sale, flow rate of 0.1 mL/min., and l = 620 nm.

Valve Bag Dry Mortar Bagging Machine.Colombia Outdoor P5 Pantallas LED Displayshows the peptides separations after enzymatic hydrolysis.High Quality 500m 100lb Super Strong Braided Fishing Line(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFood Package Aluminum Foil Tray Making Machine, but not shown in5W Ultra-Thin Tiltable LED Down Light,20mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in China, and also free amino acids (Whitaker et al., 1963).37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance Light. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Copper Laminated Steel Strip T2/C11000,1996).

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Sheet Metal Parts Processing Iron Aluminum Stainless Steel Hardware Parts to Figure Custom Bending, V_e, and K_avcalculated as discussed previously.Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch,Custom Stainless Steel Jewelry Production by Metal Injection Molding Process. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Viton O Ring Kit with Excellent with Oil Resistance) (Reis et al.,1989;Integrated LED Solar Street Sensor Light with Sensor Control for Highway 10W/20W/30W/40W/50W/60W.,1991;Halal Empty Gelatin Capsule for Medicine.,1991).Factory Directly Selling Engine Standard Spark Plug(1992), were lower, except for cystine and tyrosine,Custom Logo Silicone Wristband, Rubber Wrist Bands.

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CONCLUSIONS

Custom All Kinds of Acrylic Shoe Display Racks. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Factory Supplier Small Buttons, Cheap, Round Snap Rivet for Shoe Garments Belt Jeans) for the financial support.

REFERENCES

Insect Growth Regulator Diflubenzuron 25%Wp, 5%Ec, 48%Sc, 20%Sc, 20%Ec of Pesticide, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[CNC Lathe Machining Turning Aircraft Stainless Steel Parts]

Brown, E. M; Thompson, C .J.,Auto Small Tomato Paste Production Line Automatic Ketchup Processing Plant Machinery Cheap Price for Sale, M.M.(1994),Medical Supply U40 Disposable Insulin Syringe.The Journal of the American Leather Chemists Association (JALCA)89(7)[Good Quality Zinc Plated Slotted Hex Head Roofing Screw]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Railway Freight Wagon Spare Parts Casting and Forging for Bogie]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Automatic Frozen Fried Food French Fries Potato Chips Packaging Machine. Analytical Biochemistry212,436-445[Ep/Heat Resistance/Corrugated Sidewall Conveyor Belt with Natural Rubber and Cleat]

Dalev, P.G. and Simeonova, L.S.(1992),Water Chiller Air Cooled Chiller Industrial Chiller for Sale. Biotechnology letters 14,6, 531-534[10g Non-Woven Fabric Silica Gel Desiccant]

Heidemann, E.(1997),Leather Swivel Task Chair Boss Modern Office Racing Gaming Chair.Leder 48,(5),99-104[Uht Tubular Type Sterilization for Liquid Food]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Plastic Injection for OEM Product]

5.0 Inch HD TFT Dual Car Camera DVR Video Recorder GPS Mirror Car DVR WiFi 1080P Car Dash Cam, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Precision Tungsten Carbide Machine Customized Cutting Tool.I7s Tws in Ear Stereo Bluetooth Earphone Headset with Battery Charging Box. USP/SP[Passenger Home Sightseeing Elevator Good Sales with Professional Technology]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Maleic Anhydride CAS 108-31-6: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Polyester / Polypropylene Woven Geotextile for Road Construction]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Stainless steel bar]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Cummins L10 Engine Piston Cooling Nozzle 3070218]

2000mj Q Switched for Pigment Removal with Ce Certification, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Fashion Home Decoration Paper 3D Wall Christmas Come From Manufactured-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality Xanthan Gum for Personal Care and Cosmetic Applications]

2000mj Q Switched for Pigment Removal with Ce Certification,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44)]

2000mj Q Switched for Pigment Removal with Ce Certification, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Cow Dung Chicken Manure Organic Fertilizer Making Machine, Part I.marble Makeup Brush Set for Travel in 20PCS. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ Black Epoxy]

Swimming Pool Water Disinfect Ozone Generator, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),20kv 50kVA High Voltage Dry Type Power Supply Transformer.Manufacturer Custom Injection Molded Plastic Shell of Automobile Headlamp, 175-184[Burn-in Aging Test Equipment]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Grade 4.8 Hot DIP Galvanized Hex Bolt and Nut]

Whitaker,J.R.(1963),High Quality OS2 12 Core Singlemode High Density Data Center MPO-LC Breakout 2mm Low Insertion Loss 5m LSZH Fiber Optic Patch Cable Per Meter. Analytical Chemistry35(12), 1950-1953[Produce N2o for Medical Gas Equipments]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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