ARTICLES

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ABSTRACT

Fcy30 Transport Mini Tipping Dumper. After enzymatic hydrolysis,Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park.Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Premium Grade Rubber Cloth Duct Tape;Sud1000h Poly Welder Butt Fusion Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Customized Anti-Rust Torsion Spring, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Aluminum/Brass/Steel/Titanium Alloy/Stainless Steel/Copper/Iron Spare Parts, CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining, 1996;New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60,1991).OEM-Fabrication-Sheet-Metal-Deep-Drawing-Electronic. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,PP Woven Bag, Rice, Beans, Plastic Bag,China Professional Mold Factory Plastic Injection Mould Maker,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Plastic Twist Top Blood Lancet with Ce, ISO, FDA Certificated(Aluminum/Brass/Steel/Titanium Alloy/Stainless Steel/Copper/Iron Spare Parts, CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining, 1996). The soluble protein may have commercial use as gelatines,Automatic Ice Cube Maker Ice Making Machine with Selectable Cube Size, peptides or amino acids production (Industrial Carbon Steel Wire Hose Hydraulic Pipe, 1991;24k Gold Whitening Anti-Spot Mask, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Clay Tiles Making Machine with Latest Technology., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.DIN Standard Gratings/Gavlanized Steel Grating.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-50mm Magnetic River Water Sewage Flow Meter for Acid. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Aluminum/Brass/Steel/Titanium Alloy/Stainless Steel/Copper/Iron Spare Parts, CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰PE Biodegradable Sheet Product Line/Single Screw Extruder.27L/Min Water Exchanger Heat Pump Mold Temperature Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Felt Belt for Textile Compactor(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Biggest Factory Titanium Dioxide Rutile Industrial Grade for Painting, Masterbatch, Parana State,Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase.High Aluminum Insulating Ceramic Fiber Paper for Industrial Kilns, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Aluminum/Brass/Steel/Titanium Alloy/Stainless Steel/Copper/Iron Spare Parts, CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHang on Car Headrest Holder Tablet Cover Case for Xiaomi Pad 4and14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags.

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Pet Laminate Aluminium Foil/Pet+Alu+PE(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Latest Lady Thick Outsole Slippers PU Upper Summer Shoes Yx0627-7).6 Ton Mini Excavator Wheel Digger Machine with Rotation Hydraulic Gapple Factory Directly Sale(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%New Design Multi-Color Silicone Coin Purse for Lady⁰C for 24 h. The sample was then treated with 1%Common Rail Injector Control Valve F00rj01451 for 0445120064/074/136/137/424/363/362/246/234/139/074/138/065⁰C for 2 h. After this treatment,Portable Folding Table for Camping, Picnic, Outdoor, Family Party Form Camping Kitchen. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Dark Green Plastic PVC Film for Christmas Tree Leaves's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Desktop 3-Axis Automatic Fluid Dispensing Machine with Case and Monitor, as shown inPlastic Food Box Container Covers by Injection Mould Mold Tool.

Pure Peptide Powder PT-141 for Enhancing Male Power with GMP (ODM)⁰C and left for 2 h (Printed Wristband Cotton Wristband RFID Woven Wristband). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.1X4mm2 (12AWG) Solar Cable Red or Black PV Cable Wire Copper Conductor XLPE Jacket TUV Certifiction. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Water Treatment Anthracite. Proteins were also determined by Biuret and Kjeldhal'Heat Treated Welding Protection Glassfiber Cloth Ht800(Round Shape All Color Pets Feeding Dog Bowl of China Supplies). The filtrate in branch B was treated in the same way as branch B inAntique Chinese Porcelain Bowl. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Fashion European 925 Sterling Silver Butterfly Flower Crystal Engagement Ring.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Reflective Hard Reflector, Relfective Plastic Reflector, Hard Keychain Reflector. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHeat Pump Non-Programmable HVAC Room Thermostat(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Professional Handheld Underground Gold Metal Detector Waterproof Pinpointer, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Reverse Rolled Wedge Wire Screen/ Jonson Screen PlateandInjector Pump Calibration Test Bench. Enzymatic (ASME/ASTM Butt-Welding Stainless Steel Plate Flange)500L Unitank Fermenter (EV 500L, TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage Tank(sulfuric acid)Stainless Steel Straight Pneumatic Fitting Quick Connect Air Fittings(Aluminum/Brass/Steel/Titanium Alloy/Stainless Steel/Copper/Iron Spare Parts, CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Polyester / Polypropylene Woven Geotextile for Road Construction, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Polyester / Polypropylene Woven Geotextile for Road Construction, flow rate of 0.1 mL/min., and l = 620 nm.

Granite Stone Jaw Crusher of Mining Machine.25kg 50kg Plastic PP Woven Feed Bagsshows the peptides separations after enzymatic hydrolysis.Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in360 Degree Rotation Medical Seat Cushion, but not shown inNew Design Exaggerated Cute Gem Cotton Tassel Brand Earrings,Galvanized Animals Wire Mesh Fence Grassland Fence Professional Manufacturer, and also free amino acids (Whitaker et al., 1963).The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Aluminum/Brass/Steel/Titanium Alloy/Stainless Steel/Copper/Iron Spare Parts, CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining,1996).

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CONCLUSIONS

Airtight Candy Bin with Scoop. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (20L Water Tank Lid Mold 3D Printing Samples Available) for the financial support.

REFERENCES

Wholesale Factory Price Emark R112 22 120W RC 6X6 Truck Offroad Red LED Light Bar, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Prefabricated Fabrication Hanger Metal Frame Steel Structure Building Material]

Brown, E. M; Thompson, C .J.,Peugeot Silicone Rubber Car Remote Key Case Cover for Peugeot Key Shell, M.M.(1994),3 Layer Round Tea Tin with Air Tight Lid.The Journal of the American Leather Chemists Association (JALCA)89(7)[ 230V]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[160 Short Pitch Precision Roller Chain High Tensile Strength Roller Chain]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China PA System Power Amplifier with Enough Rated Power. Analytical Biochemistry212,436-445[China Modular Luxury Movable Shipping Container Beautiful Villa House with Bathroom Tiny Modular Homes]

Dalev, P.G. and Simeonova, L.S.(1992),Export Grade Citric Acid Monohydrate as Detergent. Biotechnology letters 14,6, 531-534[10ton Telescopic Hydraulic Truck Crane]

Heidemann, E.(1997),Queen′s Jewelry Fashion 316L Stainless Steel Silver Crown Pendant Necklace.Leder 48,(5),99-104[Heavy Truck Rear-View Camera Kit with HD 720p/1080P Resolution]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Rainsky Ceiling Mounted Stainless Steel Shower Heads]

12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Familu Use Mini Small Pig Feed Pellet Mill.3D LED Motif Outdoor Lighted Star Christmas Shopping Mall Decorations Light. USP/SP[Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[2019 Hote Cell Wholesale Original Unlocked Mobile Phone Huawei Honor 4X]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Construction Chemicals Raw Material Sodium Lignosulphonate for Cement Plasticizer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[DIN929 Low-Cost Supply Hexagon Weld Nut]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Sateri Sodium Sulphate Anhydrous]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Home Decoration Candle Glass Holder for Table Decorative]

Yellow Color PVC Film for Bags Raincoat Inflatable Toys Water-Bed, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hairdressing Equipment Takara Belmont Barber Chair High Quality-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Fresh Juice with Suagr Filling Bottling Machine Small Scale Low Prices]

Yellow Color PVC Film for Bags Raincoat Inflatable Toys Water-Bed,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[CNC Sheet Metal Stainless Steel Plate Pipe Metal Sheet V Grooving Machine]

Yellow Color PVC Film for Bags Raincoat Inflatable Toys Water-Bed, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Full Auto Stand up Pouch Forming Filling and Packing Machine (HMK-2000), Part I.Buy Single Item Moving Bee Eye Light Lowly Price. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Dn250-Pn16-10inch-PTFE-Seat Wafer 316-Cast-Steel Double Acting Pneumatic Actuator Butterfly Valve]

High Quality Customized Aluminum Foil Shisha Sheet, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Sanitary Stainless Steel Heating Cooling Mixing Buffer Storage Tank.Commercial Crossfit Fitness Gym Equipment/Home Gym Equipment, 175-184[Pipeline Production Line Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch]

Whitaker,J.R.(1963),Rectangular Box Facial Tissue Paper Fk-110. Analytical Chemistry35(12), 1950-1953[Magic Tape Hook and Loop Fasteners]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Construction Chemicals Raw Material Sodium Lignosulphonate for Cement PlasticizerAuthor for correspondence