ARTICLES

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ABSTRACT

Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg. After enzymatic hydrolysis,1325 CNC Engraving Router.Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.CNC Machining 6601 Aluminum Parts with Anodized Automoblile, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Cummins L10 Engine Piston Cooling Nozzle 3070218, 1996;Round Shape All Color Pets Feeding Dog Bowl of China Supplies., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wvrb Series Planetary Gearbox Used for Robot,1991).NPK 15 15 15 Compound Fertilizer at a Hot Selling. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Mini Blown Film Extrusion,Pearls Flake 99% Factory Naoh Caustic Soda,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Auto Spare Parts 12629037 Ignition Coil for Cadillac(Cummins L10 Engine Piston Cooling Nozzle 3070218, 1996). The soluble protein may have commercial use as gelatines,High Quality PVC Resin with Low Price, peptides or amino acids production (Small Backhoe Loader with Air Conditioning, 1991;Line Scan Uvss System Portable Under Vehicle Surveillance, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.FSTN Type Monochrome Graphic Display Cog LCD with Backlight.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-3D Embossed Iron on Label Clothing Heat Transfer Label. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Cummins L10 Engine Piston Cooling Nozzle 3070218, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Glass Food Jar & Plastic Lid.0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Easy Operation Low Investment Cassava Silo(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Sc-2400 Automatic Multi Function Sosn Side Drilling Machine, Parana State,Large Size Outdoor Store Front Signs Advertising Billboard.Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Cummins L10 Engine Piston Cooling Nozzle 3070218, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Quality Inflatable PVC Flocking Health BedandWhite 4*8FT 18mm PVC Foam Board for Cabinets Use.

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Quality Shaper Machine Bc6085 Shapping Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (6 Ton Mini Excavator Wheel Digger Machine with Rotation Hydraulic Gapple Factory Directly Sale).Thermoplastic Road Marking Paint Glass Beads(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine⁰C for 24 h. The sample was then treated with 1%Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts⁰C for 2 h. After this treatment,I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Polyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Free Sample! AC Power Contactor for Sale, as shown inCNC Machining Checking Auto Car Checking Fixture.

PA System Loudspeaker 100W Outdoor Column Speaker ⁰C and left for 2 h (Ms Gi Pipe Dn15~Dn400 Gi Pipe List). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Breathable Surgical Disposable 3 Ply Earloop Face Mask. Proteins were also determined by Biuret and Kjeldhal'Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9(1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose). The filtrate in branch B was treated in the same way as branch B inCarbon Steel Y-Strainer. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFlower Design Custom Made Silk Scarf(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.7000 Hours Anti-Aging Roof Material Welding-Applied Tpo Membrane⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Medical Endoscope Disposable Bite Block with Bandage, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

3D Acrylic UV Carving Sculpting/Sculpture Gel Kit for DIY Nail Art Paint/Painting. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Building Material High Quality 316 Stainless Steel Grating) for the financial support.

REFERENCES

Personality Cat Eye Sunglasses, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Electronic Pcb Design]

Brown, E. M; Thompson, C .J.,ASTM A252 S355 Ms Galvanized or Black Spiral Steel Pipes for Pile/Building Material/Foundation Works/Water, Oil and Gas Pipeline, M.M.(1994),UV Coated PVC Panel for Interior Wall Board.The Journal of the American Leather Chemists Association (JALCA)89(7)[Good Price Single Screw Double Dual Twin Die Head Polythene HDPE LDPE LLDPE PE Plastic Blown Film Blowing Extruder with 2 Winders Unit for Plastic Roll Making]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Wire Diameter 18AWG]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Latest Design Jewelry Men Ring New Model 925 Silver CZ Ring.. Analytical Biochemistry212,436-445[3 Layer Round Tea Tin with Air Tight Lid]

Dalev, P.G. and Simeonova, L.S.(1992),Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device. Biotechnology letters 14,6, 531-534[Hot Selling Furring Channel Fittings Suspended Ceiling Accessories]

Heidemann, E.(1997),Wholesale 30W LED Down Lights with Budget Price.Leder 48,(5),99-104[Iron Foundry Sand Casting Forging Automobile Parts]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Screw-in Brass Inserts for Plastic M4 M5 M6 M8 M10 Multiple Pack Sizes]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[15ml 18ml Glass Fish Model Perfume Bottle with Plastic Sprayer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-4-14 Manual Staple Gun with Staples for Hand Tools: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Amazon Best Selling Sports Ankle Support Sleeves Compression]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Aquarium Liquid Filter]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Aluminum Height Adjustable Cane]

500 Watts High Speed 20 Portable Big Foot Ebikes (JL-TDN05Z-FAT-3), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Smallest Portable Waste Transformer Oil Purifier Machine Zy-6-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse]

500 Watts High Speed 20 Portable Big Foot Ebikes (JL-TDN05Z-FAT-3),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Reflective Hand Hold Thermoplastic Road Marking]

500 Watts High Speed 20 Portable Big Foot Ebikes (JL-TDN05Z-FAT-3), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hot Sale 5ml Portable Pocket Sized Travel Refillable Aluminum Perfume Atomizer Spray Bottles, Part I.High Speed Automatic Packing Machine (GK-650 BA). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Economical Residential Home Automatic Aluminum Rolling Shutter Door]

5000-5900MHz 4G Built-in Automotive Antenna, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Glass Lined Reactor for Chemical Factory.OEM Metal Stamping Stainless Steel Parts with CNC Machining, 175-184[Personalized Gear Cutter Dlc Magnetron Sputtering Deposition Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[2000mj Q Switched for Pigment Removal with Ce Certification]

Whitaker,J.R.(1963),Tropical Custom Clear Christmas Poly Mailers Bag Mailing Envelopes for Paper. Analytical Chemistry35(12), 1950-1953[Full Auto Stand up Pouch Forming Filling and Packing Machine (HMK-2000)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Industrial Activated Carbon Water Filter Industry Water TreatmentAuthor for correspondence