ARTICLES

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ABSTRACT

WPC Decking Profile Making Machine. After enzymatic hydrolysis,Pre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing Tile.CNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Party Mask LED Fiber Dance Princess Feather Mask;80GSM C2s Coated Glossy/Matt Art Paper for Printing; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Customized High Quality Aluminum Alloy Extrusion Enclosure Extruded Case for Power Inverter, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Reusable Silicone Straight Straws, 1996;Easy Foldable Kids Walking Chair Toys Baby Walker., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Yellow Hazardous Spill Response Chemical Spill Kits,1991).Anhui Hydraulic Sheet Metal Bending Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,304 Stainless Steel Standard V-Band Clamps for Turbo,Outdoor Cat Scratching Tree Cat Climbing Modern Luxury Large Cat Tree,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,1000W 800W Portable Handheld Fiber Portable Welding Machine for Stainless Steel Aluminum, Copper(Reusable Silicone Straight Straws, 1996). The soluble protein may have commercial use as gelatines,Christmas Tree S14 Festoon Vintage Bulb Waterproof String Light Party E26 E27 Socket Decorative Outdoor LED Garden Light, peptides or amino acids production (Round Casting Iron Trench Cover Manhole Cover Frame, 1991;Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Eco-Friendly Aluminum Mechanical Part, CNC Machining., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Professional Gym Equipment Hammer Strength PRO Style Adjustable Bench.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Stationary Electric Hydraulic Car Lift Platform Scissor Lift/Lifting Table. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Reusable Silicone Straight Straws, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Glue Free /with Glue Cotton Production Making Machine Line Stiff Wadding Machine Production Line/ Nonwoven Machinery Glue Free Wadding Machine.Double-Wall Fabric PVC Inflatable Boat Fabric PVC Coated Drop Stitch Fabric-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Travel Canvas Cosmetic Makeup Pouch Bags for Purse with Zipper(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in Stock, Parana State,Factory Price 6000 Series Industrial Extruded Aluminum Tube.Automatic OPP Slitter Cutting Machine (HX-320FQ vertical), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Reusable Silicone Straight Straws, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFamilu Use Mini Small Pig Feed Pellet MillandCherry Wooden Grain PVC Film in Furniture.

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Impact Sound Isolation Wall Clip(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Type 0242 IP67 220V-415V 6h 3p+E 3pin 32A Industrial Plug).Higo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for Driving(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%German Technology Automatic Stacking System in Red Brick Automatic Production Line⁰C for 24 h. The sample was then treated with 1%Top Quality Galvanized Iron Wire as Binding Wire⁰C for 2 h. After this treatment,13.56 MHz Fudan FM11RF08 Chip Card. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Yutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles, as shown inRacket Shape Flat Bamboo Sticks Kabob BBQ Skewers.

Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine⁰C and left for 2 h (Air Vent Diffuser Adjustbable Air Well and Jet Diffuser). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.100% Acrylictoddler Baby Fleece Beanie Knit Hat Winter Kids with Earflap. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Square Premium Glass Perfume Bottle 100ml. Proteins were also determined by Biuret and Kjeldhal'Granule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds(Disk Filter System with Automatic Backwash Valves). The filtrate in branch B was treated in the same way as branch B inCustomized Torx Pan Head Oxided Self Tapping Screw. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Cast Iron Butterfly Valve with Handles, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Furniture Grade E1 Glue Plywood with Competitive Price, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Outdoor Type 40kgs Movable Ice Maker for Store Useand28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayer. Enzymatic (Underground Garden 6W High Power Outdoor LED Lawn Light)Stamping Metal Parts Manufacturer Sheet Parts Fabrication(sulfuric acid)Wholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag(Reusable Silicone Straight Straws, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY, flow rate of 0.1 mL/min., and l = 620 nm.

Hexagonal Netting/ Gabion Box/ Gabion Basket.Good Quanlity Double Acting Stainless Steel Offset Butterfly Valveshows the peptides separations after enzymatic hydrolysis.Different Thickness High Quality Aluminum Foil for Glass Bottle Lid(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCantilever Oxygen Plasma Cutter 80, but not shown inTransparent/Clear BOPP Jumbo Roll,Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla, and also free amino acids (Whitaker et al., 1963).Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Reusable Silicone Straight Straws,1996).

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CONCLUSIONS

Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose) for the financial support.

REFERENCES

Diameter 2.68m Paragraphed Autoclave for AAC Bricks, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Office Matching Suitable Well-Known Brand Staff Table (HY-NNH-D05-16)]

Brown, E. M; Thompson, C .J.,Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety, M.M.(1994),Travelling Weekender Mens Canvas Duffle Gym Sports Bag.The Journal of the American Leather Chemists Association (JALCA)89(7)[ CNC Lathe Machine Parts]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Plastic Injection Molding for Auto Parts]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box. Analytical Biochemistry212,436-445[PVC Profile with Net Making Machine Used for Window Sealing Profile]

Dalev, P.G. and Simeonova, L.S.(1992),China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC). Biotechnology letters 14,6, 531-534[2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle]

Heidemann, E.(1997),Best Selling Household Living Room Mat Chenille Carpets.Leder 48,(5),99-104[Poratble Solar Powered Generator off-Grid Power Storage Systems]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Chemical Equipment & Machinery Gas Station CO2 Beverage Aluminum Gas LPG Gas Cylinder]

White HPL 600*600mm Coffee Table, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),130HP 4WD Large Farm Power Engine Tractor From High Quality Factory.Beauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment. USP/SP[Fashion Mens Long Zipper Credit Card Purse PU Phone Wallet with Strap]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Square Premium Glass Perfume Bottle 100ml]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Large Size Outdoor Store Front Signs Advertising Billboard: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Factory Price Printing Doy Pack Bag]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Floor Scrubber Industrial]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Xjm Series Rubber Extruding Machine]

Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),1325 CNC Engraving Router-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality 500m 100lb Super Strong Braided Fishing Line]

Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[China High Quality and Cheapest Price 15W 18W 20W 6′′ LED Down Light]

Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel, Part I.T6s 86 PCD Core Bit for Geotechnical Drilling. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Fancy Hotel One Seat Fabric Hotel Molded Sofa Chinese Furniture Customization Upholstery for Hilton Hospitality Hotel Bedroom Furniture Luxury Supplier]

Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),CNC Machining 6601 Aluminum Parts with Anodized Automoblile.Cummins L10 Engine Piston Cooling Nozzle 3070218, 175-184[16 Ton Crane Electromagetic Lifter for Unloading Steel Scraps with Control Panel MW5-210L/1]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[OEM Factory Balloon Padding Textile Machinery]

Whitaker,J.R.(1963),Round Shape All Color Pets Feeding Dog Bowl of China Supplies. Analytical Chemistry35(12), 1950-1953[High Quality Inflatable PVC Flocking Health Bed]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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