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ABSTRACT

Heavy Duty 90 Degree Large Decorative Table Iron Metal Stainless Steel Adjustable Angle Shelf Wall Mounted Metal Table Folding Bracket. After enzymatic hydrolysis,Disposable Non-Woven Dry Gloves Wipes Making Machine.ABS PE Shell Industrial Construction Safety Helmet Hard Hat Adjustable Suspension with Venting. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Waterproof Exterior High-Pole 1000W LED Sport Court Flood Lighting 120lm/ IP65;Bottom Pushing for Fire Extinguisher Hot Spinning Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fish Scales Texture Fashion Leather for Shoe (S312100GH), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Diamond Segments 2000mm Diameter Granite Cutting Blade, 1996;Produce N2o for Medical Gas Equipments., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot Selling Furring Channel Fittings Suspended Ceiling Accessories,1991).90m3 Per Hour Mobile Concrete Pump. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China High Quality CATV FTTH 1550 Nm External Modulated Optical Transmitter,FTTB Wdm Optical Receiver with 2 Output / AGC FTTB Optical Node,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,All Weather Rattan Handwoven Swing Outdoor Home(Diamond Segments 2000mm Diameter Granite Cutting Blade, 1996). The soluble protein may have commercial use as gelatines,Pond Self Priming Booster suction  Water Pump with Pressure Switch, peptides or amino acids production (Digtal Camera for Sewer Line Inspection System with Self Level and Meter Counter, 1991;Modern Furniture Brick Finishing Wood Fireplace Mantel Surround Electric Fireplace, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;for Wall Decoration Glass Silver Metal Aluminium Mosaic Tile., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.100% Polyester Gabardine Shirting Fabric with Transfer PrintingTree.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-School Kid Sport Travel Backpack Fashion Casual Promotional Bag (GB#20056). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Diamond Segments 2000mm Diameter Granite Cutting Blade, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Fuguang High Quality Fb1015-280 280ml Double Wall Glass Water Bottle.Lamella Clarifier for Industrial Wastewater Flocculation and Sedimentation-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Wholesale High Pressure 80L Nitrogen Gas Cylinder, Parana State,Wholesale Interior Metal 3D Bee Garden Ornament Decoration.10mm Red Green Yellow Warning China Supplier Signal Lamp, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Diamond Segments 2000mm Diameter Granite Cutting Blade, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theMotorcycle Accessories with MIM Part by Metal Injection Molding Technology with Precision and Complex Metal PartsandKorean Integrated CTO Activated Carbon Filter for Water Treatment.

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Ms-184125-2 1.3mh Arnold Core Common Mode Choke Coils Toroidal Inductor(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (0.5 Gallons Cool Mist Ultrasonic Humidifier).Hot Sale Thermostat Electric Heating Element for Solar Water Heaters(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Phillip Countersunk Head Screw Hot DIP Galvanized⁰C for 24 h. The sample was then treated with 1%Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse⁰C for 2 h. After this treatment,Cow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Heat Shrink Automatic Flow Wrapping Packing Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam, as shown in1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Price.

Ductile Iron Standard Brackets Pipe Hanger⁰C and left for 2 h (Promotional Customized PP Hand Fan). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Luxury Vintage Throw Pillows Cover for Couch, Damask Pillows Cover, Decorative Pillow Covers. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper. Proteins were also determined by Biuret and Kjeldhal'5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building(Auto Plastic Part Hardware Plastic Injection Mold). The filtrate in branch B was treated in the same way as branch B inCondensing Unit With Air Handles. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality Sheet Metal Bending Roll Forming Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Different Size Glass Test Tubes, High Quality Laboratory Test Tubes. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avProfessional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Kitchen Metal Dish Storage Rack (JP-SC984C)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming, pH 3.25., mobile phase B: buffer solution of 0.01 mol/AVR Se350 Automatic Voltage Regulator for Marathon Alternator, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Threaded Stainless Steel Motorized Electric Floating 3PC Ball Valve.Chemical Plant Transfer Industrial Water Pumpshows the peptides separations after enzymatic hydrolysis.Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSMS 25g Non Woven Isolation Gown with Knitted Cuff for Hospital, but not shown inBest Seller Hot Sale Compatible Lemos Feg 2b 26 Pin Metal Circular Push Pull Self Latching Connector,LED T20 Ba15D Indicator Lamps for Electrical Equipments, LED Ba15s/Ba15D Auto Bulbs, LED Bayomet Vehicle Lamps, and also free amino acids (Whitaker et al., 1963).New Style Polyester Duffle Bag for Travelling Trolley Bag Luggage Bag with 2 Wheels. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Diamond Segments 2000mm Diameter Granite Cutting Blade,1996).

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CONCLUSIONS

Digger Arm Small Wheel Loader (HQ908) with CE. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Fashion Large Capacity Transparent PVC Leather Handbag Tote Shopping Bag) for the financial support.

REFERENCES

15ml 18ml Glass Fish Model Perfume Bottle with Plastic Sprayer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cigarette Head Coil]

Brown, E. M; Thompson, C .J.,Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output, M.M.(1994),Water Based Emulsion Paint Production Line.The Journal of the American Leather Chemists Association (JALCA)89(7)[Manufacturer of Soda Ash Dense 99 2% Price]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Barbed Wire Right off The Border Wall to Protect Homes]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Cnm Single Strand Anchor Coupler. Analytical Biochemistry212,436-445[New Design 4*2 Street Cleaning Machine Sweeping Truck with Brushes]

Dalev, P.G. and Simeonova, L.S.(1992),Wisdom LED Floor Lamp, Head Lamp Fabia Mining Lamp. Biotechnology letters 14,6, 531-534[Magnifying Mirror Shaving Mirror 2X]

Heidemann, E.(1997),18X16mesh Fiberglass Window Screen Insect Screen.Leder 48,(5),99-104[Clothing Apparel OEM Slim-Fit Men′s Flat Front Active Waistband Golf Shorts]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder]

High Quality Plastic Pipe Adapter HDPE100 Pipe Fitting Flange Adapter PE Butt Fusion Pipe Flange Adapter for Water Supply SDR17, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch.Sterile Dsiposable Syringe for Dental 12ml. USP/SP[Outdoor Concert Stage Equipment Aluminum Stage Portable Stage]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Foldable Bed Military Cot for Camping: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Laser Aluminium Sheet，Aluminium Foil Sheet，Embossed Aluminium Sheet，Aluminium Sheet For Bottle Cap]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine]

P5 960*960mm LED Display Screen SMD Indoor /Outdoor, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),2019 New Advertising Inflatables Air Walking Backpack Ball-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[FTTX Solution Provider Fiber Optical 12cores MPO Cassette]

P5 960*960mm LED Display Screen SMD Indoor /Outdoor,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Latest Design Jewelry Men Ring New Model 925 Silver CZ Ring.]

P5 960*960mm LED Display Screen SMD Indoor /Outdoor, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Side Pack Manual Gas Engine Power Brush Cutter, Part I.Punching Bending Welding Steel Work Fabrication. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Waterproofed 4WD Boat Truck Winch 12000 Lbs 12 V Electrical Cable Winch Ce Approved]

Black and White Cat Mobile/Cell Phone Cover/Case for iPhone 6/7/6plus/7plus, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Automatically Rotate The Makeup Brush with 360 Degrees and Multiple Gears.1 Ton Chain Pulley Block with Geared Trolley, 175-184[Wholesale Top Sell Comfortable Sample Style Latest Corner Sofa Design]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hot Sale Instax Mini Camera Korea Style Photo Album]

Whitaker,J.R.(1963),Powder Coating Polyester Resin with Factory Price. Analytical Chemistry35(12), 1950-1953[Sterile Dsiposable Syringe for Dental 12ml]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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