ARTICLES

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ABSTRACT

Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam. After enzymatic hydrolysis,Ductile Iron Standard Brackets Pipe Hanger.Luxury Vintage Throw Pillows Cover for Couch, Damask Pillows Cover, Decorative Pillow Covers. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Sheet Metal Bending Roll Forming Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Different Size Glass Test Tubes, High Quality Laboratory Test Tubes, 1996;Professional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Frame Type Rubber Hydraulic Press Machine,1991).Kitchen Metal Dish Storage Rack (JP-SC984C). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming,AVR Se350 Automatic Voltage Regulator for Marathon Alternator,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage(Different Size Glass Test Tubes, High Quality Laboratory Test Tubes, 1996). The soluble protein may have commercial use as gelatines,Excavator Heavy Duty Bolt on Right Hand Sidecutter 232-2147, peptides or amino acids production (High Strength Rigid PVC Plastic Foam Board for FRP, 1991;Round, Hexagonal Hole Stainless Steel Perforated Metal Mesh for Building, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Threaded Stainless Steel Motorized Electric Floating 3PC Ball Valve.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Different Size Glass Test Tubes, High Quality Laboratory Test Tubes, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰LED T20 Ba15D Indicator Lamps for Electrical Equipments, LED Ba15s/Ba15D Auto Bulbs, LED Bayomet Vehicle Lamps.New Style Polyester Duffle Bag for Travelling Trolley Bag Luggage Bag with 2 Wheels-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High Quality Ceramic Chrome Hybrid Bearing Oil, Parana State,Heat Shrink Automatic Flow Wrapping Packing Machine.Medical Product Nonwoven Disposable Surgical Gown PP Isolation Gown, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Different Size Glass Test Tubes, High Quality Laboratory Test Tubes, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBlank Personalized Engraved Plaques Name Metal Art Crafts Plating Copper 3D Marathon Sports Medal with Customized Design (MD04-C)andPrefab Building Insulated Interior Concrete Partition Walls.

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CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (3120/17/38 Boltable Crane Rail Clips).Digger Arm Small Wheel Loader (HQ908) with CE(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%15ml 18ml Glass Fish Model Perfume Bottle with Plastic Sprayer⁰C for 24 h. The sample was then treated with 1%Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output⁰C for 2 h. After this treatment,Water Based Emulsion Paint Production Line. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Cnm Single Strand Anchor Coupler's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wisdom LED Floor Lamp, Head Lamp Fabia Mining Lamp, as shown inMetallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machinery.

18X16mesh Fiberglass Window Screen Insect Screen⁰C and left for 2 h (P-Level Sp-Level Mute Interchangeable Linear Motion Guide Rails). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Sterile Dsiposable Syringe for Dental 12ml. Proteins were also determined by Biuret and Kjeldhal'Foldable Bed Military Cot for Camping(Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device). The filtrate in branch B was treated in the same way as branch B inElectric Galvanized Crimped Wire Mesh. The final soluble protein shown were used for peptides separations using gel permeation chromatography,P5 960*960mm LED Display Screen SMD Indoor /Outdoor.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%2019 New Advertising Inflatables Air Walking Backpack Ball. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSide Pack Manual Gas Engine Power Brush Cutter(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Black and White Cat Mobile/Cell Phone Cover/Case for iPhone 6/7/6plus/7plus⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Automatically Rotate The Makeup Brush with 360 Degrees and Multiple Gears, pH 3.25., mobile phase B: buffer solution of 0.01 mol/1 Ton Chain Pulley Block with Geared Trolley, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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180W 1850mm*80mm Reci Laser Tube.Jacquard Football Fans Acrylic Sports Warmer Scarfshows the peptides separations after enzymatic hydrolysis.OEM Factory Balloon Padding Textile Machinery(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Efficiency Multistage Diesel Centrifugal Vane Pump for Chemical Industry, but not shown inCrop Fertilizer Agriculture Nh4cl Ammonium Chloride,NdFeB Cylindrical Magnets Coated with Holes  Zn, Ni, Black Epoxy, and also free amino acids (Whitaker et al., 1963).Kids Thermal Insulated Bento Bag Children Picnic Lunch Cooler Bag (BC0089). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Different Size Glass Test Tubes, High Quality Laboratory Test Tubes,1996).

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China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adultsand Viola Bowshow the gel chromatographic separation of soluble protein with Sephadex G-Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine.Outdoor Concert Stage Equipment Aluminum Stage Portable Stage, and another peak at 45 mL not shown inElectrical 4W AC Synchronous Gear Motor for Engraving Machine, for peptides lower than 3000 Da.Invt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Home(Condell et al.,1993) .

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CONCLUSIONS

Custom Anodized Aluminum Bending Parts Sheet Metal Stamping Parts. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap) for the financial support.

REFERENCES

P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Electric Wire 1.5mm]

Brown, E. M; Thompson, C .J.,Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine, M.M.(1994),Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings.The Journal of the American Leather Chemists Association (JALCA)89(7)[Cross Stripe Sleeveless Yoga Wear]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[OEM Factory/Chrome Steel/ISO Certificate/Used in Machine/Pillow Block Bearings/Bearings/Bearing (UCP)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Metallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machinery. Analytical Biochemistry212,436-445[Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon]

Dalev, P.G. and Simeonova, L.S.(1992),Printed Wristband Cotton Wristband RFID Woven Wristband. Biotechnology letters 14,6, 531-534[Precision Engineering Parts Bearing Support Unit for Ball Screw]

Heidemann, E.(1997),High Quality Wholesale Clear 50ml Flat Square Crimped Glass Bottles.Leder 48,(5),99-104[15-80mm Welding Right Angle Throttle Stop Check Valve for Refrigeration Unit]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Discussion and Interpretation System]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[12V Electric Portable Outdoor Water Fruit Car Fridge with Ce]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-ASTM A240 321 Stainless Steel Sheet Price Per Kg: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Queen′s Jewelry Fashion 316L Stainless Steel Silver Crown Pendant Necklace]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.gmcmagnet.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[100% Polyester Gabardine Shirting Fabric with Transfer PrintingTree]

V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Promotional Plain Silver Empty Hinged Rectangular Tin Box-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Copper Laminated Steel Strip T2/C11000]

V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Rubber Wrist Bands]

V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling, Part I.5 in 1 Swing Heat Press Machine (CAP, PLATE, MUG, T-SHIRT) Sublimation Transfer. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Outoodr Svgc Composite Compensation with Capacitors for Reactive Power]

Butyl Glue Self-Adhesive Tape with Radian, Caulk Strip Trim Tape (38mm), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),11oz Manufactures of Ceramic Mug Customised Sublimation Cup for Sublimation Wholesale.Vertical Type Low Pressure Injection Molding Machine with After-Sales Services (JX-900H), 175-184[Cold Rolled Carbon Seamless Steel Pipe for Shock]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Water Filter Polyester Non-Woven Fabric]

Whitaker,J.R.(1963),Cantilever Oxygen Plasma Cutter 80. Analytical Chemistry35(12), 1950-1953[Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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