ARTICLES

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ABSTRACT

OEM Custom Sand Casting Supply Square and Round Ductile Cast Iron Manhole Cover and Drain Grating. After enzymatic hydrolysis,Aluminum Foil Heat Shield Screen Matting Adhesive Backed.Home Kitchen Water Purification Ozone Generator 1000mg. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;PP Foam Stationary Sheet Making Machine;Linear Guideway Flat Bed CNC Lathe Machine (BL-Z0640); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Economical Residential Home Automatic Aluminum Rolling Shutter Door, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Clear PVC Sheet with Coating for Card, 1996;Classic Black and White Plaid Sling Bag for Women., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (24 Fiber MPO MTP Breakout Cassette with MPO to LC Cable,1991).10L/15L/22L/32L/42L/52L Portable Camping Fridge Compressor Beverage Lcebox Cooler Box Chest Freezer Car Refrigerator Mini Fridge for Car/Home/Outdoor. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Free Sample E Liquid/E-Juice for Nicotine Vaping,Colored Foil Paper Hot Stamping Heat Transfer Print Package Craft,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,100% Acrylic Knitted Sports Woven Scarf(Clear PVC Sheet with Coating for Card, 1996). The soluble protein may have commercial use as gelatines,Prefab Steel Structure Shed for Storage Warehouse, peptides or amino acids production (Glass Manufacturer/Float Glass/ Tempered Glass (Flat or Curved) for Building / Furniture, 1991;Gen 2+ or 3 Day/Night Vision Scope, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Waterproofed 4WD Boat Truck Winch 12000 Lbs 12 V Electrical Cable Winch Ce Approved., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.3pH in 3 pH out High Frequency Online UPS - Modular UPS 10 -300kVA.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-1650mm*80mm Stable Power Laser Tube. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Clear PVC Sheet with Coating for Card, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰FDA ISO Ce Marked Medical Diagnostic Dengue Rapid Test Kit.High Quality Lead Ingot 99.994 Reasonable Price-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Micro-Combustion Tube, Test Tube (HK-224)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Stationery PP Clipboard With 7 Inch Scale Ruler, Parana State,Fancy Veneer Plywood. Natural Veneer Plywood for Furniture.Automatic Food Cookies Cake Cartoning Machine for Big Size Carton Box, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Clear PVC Sheet with Coating for Card, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theMedical Supply U40 Disposable Insulin SyringeandManhole Galvanized Steel Trench Cover for Drain.

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Custom Blue Round Lid Spray Perfume Glassware Cosmetic Glass Bottle(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Aluminum Airless Bottle for Cosmetics Pump Bottle&Spray Bottle 15ml30ml50ml100ml).PVC Cross Joint Pipe Fittings Equal Pipe Cross(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%3PC Stainless Steel Flanged End Ball Valve 3 Inches 150⁰C for 24 h. The sample was then treated with 1%Ysdcnc Manual Sheet Metal Plate Bending Roller Machine⁰C for 2 h. After this treatment,Betaine Anhydrous 98% Food Grade Good Quality Competitive Price. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)2019 Brand New Promotion Gift Custom Logo Printed Laptop Webcam Cover's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Bearing Stand Type Cam Follower, as shown inCanton Fair Galvanized Steel Aluminium Ibr Roof Sheet Roll Forming Making Machine.

Hot Sale Electric LED light Aroma Diffuser⁰C and left for 2 h (Customized Made EVA Swim Kickboard Swimming Kickboard Swim Kick Board Swim Kickboard Swim Board). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Wear Resistant Rubber Ceramic Wear Linier Plate for Chute Liner. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,DC 12V Wireless Remote Control Gate Garage Duplicator Jh-Tx306. Proteins were also determined by Biuret and Kjeldhal'Mini Shampoo Plastic End Caps Cosmetic Packaging Screw Caps(Custom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole). The filtrate in branch B was treated in the same way as branch B in55g Carbonless NCR Colored Copy Printing Paper. The final soluble protein shown were used for peptides separations using gel permeation chromatography,7PCS RGBW LED Plastic PAR Light Wall Washer.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%750W 8X16 Precision Mini Metal Lathe 3 Jaw 50-2500rpm. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avKindergarten Playground Preschool Kids Plastic Slide and Swing(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Railway Freight Wagon Spare Parts Casting and Forging for Bogie, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Rainsky Ceiling Mounted Stainless Steel Shower Heads, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (3W LED Explosion Proof Emergency Exit Light) for the financial support.

REFERENCES

Tumble Belt Type Shot Blasting Machine for Iron Castings Parts, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Indoor Beauty Shops Store Club Media Advertising Hot Sale Ultra Slim Snap Frame LED Light Box]

Brown, E. M; Thompson, C .J.,Bsp Standard Malleable Iron Pipe Fitting Hexagon Nipple, M.M.(1994),Good Quality Good Price China Plastic Injection Molding Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Modern Vertica Parking Garage System]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Luxury Aroma Reed Diffuser with Rattan Sticks for Decoration]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Pet Hair Remover Glove for Dog, Grooming Gloves Brush. Analytical Biochemistry212,436-445[Color Cardstock Color Paper Manufacturer]

Dalev, P.G. and Simeonova, L.S.(1992),Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa. Biotechnology letters 14,6, 531-534[Wholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag]

Heidemann, E.(1997),Alumina Balls for Hydogen Peroxide.Leder 48,(5),99-104[Star Led Candle Light]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Manual Sealing Machine for Instant Noodles Paper Soup Bowls]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Lm Series Miniature Lm8uu Liner Ball Bush Bushing Ball Bearing]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-DIN127 Stainless Steel Spring Lock Washer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Aluminium CNC Precision Turned Machining Parts]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Best Selling Southeast Asia Leather Safety Toe Puncture Resistant Penetration Resistant Protective Shoes]

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29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boom,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Competitive Price Good Quality Custom Unisex Marle Grey Fleece Hoody]

29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boom, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System, Part I.Automotive Bearings, Wheel Hub Bearing, Wheel Bearing (387144). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Magnetic Mixer Mixing Tank with Bottom Agitator (MTK)]

Garlic Red Mesh Bag, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Z60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart Reminder.Ground Finish Molybdenum Tzm Rod, 175-184[Rope Suspended Platform Aluminum Alloy Electric Swing Stage Hoist]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Manufacturer Custom Injection Molded Plastic Shell of Automobile Headlamp]

Whitaker,J.R.(1963),Transparent/Clear BOPP Jumbo Roll. Analytical Chemistry35(12), 1950-1953[ Gym Sport Backpack]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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