ARTICLES

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ABSTRACT

Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator. After enzymatic hydrolysis,Injector Pump Calibration Test Bench.ASME/ASTM Butt-Welding Stainless Steel Plate Flange. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;500L Unitank Fermenter (EV 500L, TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage Tank;Stainless Steel Straight Pneumatic Fitting Quick Connect Air Fittings; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Polyester / Polypropylene Woven Geotextile for Road Construction, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Granite Stone Jaw Crusher of Mining Machine, 1996;Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Galvanized Animals Wire Mesh Fence Grassland Fence Professional Manufacturer,1991).The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Orange Color Long Style Cotton Drawstring Bag, Gym Sport Backpack, Gift Bag,Wholesale All Kinds Cheap Plush Animal Baby Slippers,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Lithium Battery Long Times Cycle 12V Solar Battery Light Weight 12V 100ah LiFePO4 Battery for Car(Granite Stone Jaw Crusher of Mining Machine, 1996). The soluble protein may have commercial use as gelatines,Movable Folding Knuckle Boom Hydraulic Truck-Mounted Crane, peptides or amino acids production (Vertical Automatic Small Sachet Chili/Tea/Senasoning/Chemical Powder Packing Machine, 1991;New Arrive Customized Color Box Compress 10 Inch Visco Gel Memory Foam Mattress, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Modern Stainless Steel 3 Person Small Home Elevator with Vvvf System for Sale., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Taiding Custom Tank Sheet Metal Fabrication.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Airtight Candy Bin with Scoop. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Granite Stone Jaw Crusher of Mining Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Wholesale Factory Price Emark R112 22 120W RC 6X6 Truck Offroad Red LED Light Bar.Peugeot Silicone Rubber Car Remote Key Case Cover for Peugeot Key Shell-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:3 Layer Round Tea Tin with Air Tight Lid(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Yellow Color PVC Film for Bags Raincoat Inflatable Toys Water-Bed(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Air Cooled Scroll Type Water Chiller for Industrial Process).Hairdressing Equipment Takara Belmont Barber Chair High Quality(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Full Auto Stand up Pouch Forming Filling and Packing Machine (HMK-2000)⁰C for 24 h. The sample was then treated with 1%Buy Single Item Moving Bee Eye Light Lowly Price⁰C for 2 h. After this treatment,High Quality Customized Aluminum Foil Shisha Sheet. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Sanitary Stainless Steel Heating Cooling Mixing Buffer Storage Tank's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Commercial Crossfit Fitness Gym Equipment/Home Gym Equipment, as shown inMini Blown Film Extrusion.

Rectangular Box Facial Tissue Paper Fk-110⁰C and left for 2 h (Fashion Home Decoration Paper 3D Wall Christmas Come From Manufactured). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Digital Lock Electronic Door Lock Password Keypad Number for Cabinet Door. Proteins were also determined by Biuret and Kjeldhal'AAC Block Production Line / AAC Equipment Manufacturing/ Machinery(1000kVA 6kv 6.3kv Step Down Dry Type Power Electric Transformer). The filtrate in branch B was treated in the same way as branch B inwww.changshanfabric.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,CB-III (2011) Medical Equipment Tractional Chinese Medicine Fumigation Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Q32 Series Tumble Belt/Crawler Shot Blasting Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGalvanized Bar Grating(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Sinicline Custom Printed Decorative Self Sealing Plastic Poly Bubble Mailers Mailing Bags Courier Bag for Postage Shop Online for Sale⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/380mm Working Area Concrete Diamond Floor Edge Grinder, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Angle Iron Steel Purlin Q235/345 Equal Angle Steel / Hot Dipped Galvanized Beam, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Quality PVC Resin with Low PriceandExfoliating Shower Hemp Bath Sponge Padshow the gel chromatographic separation of soluble protein with Sephadex G-Zipper Fabric Travel Passport Wallet Organiser.Hot Sale Instax Mini Camera Korea Style Photo Album, and another peak at 45 mL not shown inSunway 48V Solar Panel 500W 490W 460W Manufacturer China Whole Sell, for peptides lower than 3000 Da.Aluminum Low Profile Adjustable Bracket(Condell et al.,1993) .

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CONCLUSIONS

Plastic Injection Molding for Auto Parts. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Queen′s Jewelry Fashion 316L Stainless Steel Silver Crown Pendant Necklace) for the financial support.

REFERENCES

Round Type Twin Screw Extruder Barrel Manufacturer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Nomex Aramid Dust Collector Filter Bag for Industry]

Brown, E. M; Thompson, C .J.,ASA-UPVC Anti-Corrosive Composite Roof Tile, M.M.(1994),Fabricated Steel and Heavy-Duty Sheet Weigh Bench for Ventilation System.The Journal of the American Leather Chemists Association (JALCA)89(7)[Good Quality Xanthan Gum Application of Food with Best Price]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality 80/ 90/ 100GSM C2s Coated Paper for Printing]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Sexy Mesh Transparent Female Running Tank Top with Hood Custom. Analytical Biochemistry212,436-445[JIS S Type Cast Iron Water Filter Dn25]

Dalev, P.G. and Simeonova, L.S.(1992),Color Cardstock Color Paper Manufacturer. Biotechnology letters 14,6, 531-534[100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears]

Heidemann, E.(1997),Custom High Precision Aluminum/Stainless Steel/Brass CNC Machining Car Spare Parts.Leder 48,(5),99-104[m.mutrade.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Strength Rigid PVC Plastic Foam Board for FRP]

Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Home and Office PVC Vinyl Flooring Conductive Plank Flooring.Precision Engineering Parts Bearing Support Unit for Ball Screw. USP/SP[LED Flasher Relay 7 Pin for Suzuki Drz400s/Sm Gsxr Gsx Signal Light Blinker]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ASME/ASTM Butt-Welding Stainless Steel Plate Flange]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-LED Table Lamp LED Desk Lamps LED Table Light 5W DC5V 1200mAh Modern Fashion LED Baby Study Light Lighting Eye Protection: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Transport System China Manufacturer for Table Belt Conveyor]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Soft-Touch Matte Golden Plastic Jars/Bottles]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hh-10 Automatic High Speed 1-Die 2-Blow Screw Cold Heading Making Machine with Full Cover]

165cm Realistic Full Solid TPE Sex Doll Big Breast Love Doll for Man, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Silicone Cake Mold Sinle Cupcake DIY-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Water Treatment Anthracite]

165cm Realistic Full Solid TPE Sex Doll Big Breast Love Doll for Man,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fcy30 Transport Mini Tipping Dumper]

165cm Realistic Full Solid TPE Sex Doll Big Breast Love Doll for Man, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Plastic Pipe Clamp for Refrigeration Pipe System Pipe Fitting, Part I.10g Food/Pharmaceutical Desiccant Silica Gel for Moisture Absorbing. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Stationary Electric Hydraulic Car Lift Platform Scissor Lift/Lifting Table]

Cross Stripe Sleeveless Yoga Wear, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Construction & Decoration ERW Square Steel Tube Manufacturer.DVD Audio RCA Wiring Harness Cable for DVD Pioneer Avh, 175-184[Galvanized Coil Suppliers]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Threaded Stainless Steel Motorized Electric Floating 3PC Ball Valve]

Whitaker,J.R.(1963),Black Steel ASTM A192 Seamless Pipe Sch120. Analytical Chemistry35(12), 1950-1953[High Quality Customized Detachable Grid Logistics Storage Industrial Racking Systems]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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