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ABSTRACT

Plastic Injection Molding for Auto Parts. After enzymatic hydrolysis,Round Type Twin Screw Extruder Barrel Manufacturer.ASA-UPVC Anti-Corrosive Composite Roof Tile. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Color Cardstock Color Paper Manufacturer, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Custom High Precision Aluminum/Stainless Steel/Brass CNC Machining Car Spare Parts, 1996;Home and Office PVC Vinyl Flooring Conductive Plank Flooring., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Precision Engineering Parts Bearing Support Unit for Ball Screw,1991).LED Table Lamp LED Desk Lamps LED Table Light 5W DC5V 1200mAh Modern Fashion LED Baby Study Light Lighting Eye Protection. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,165cm Realistic Full Solid TPE Sex Doll Big Breast Love Doll for Man,Silicone Cake Mold Sinle Cupcake DIY,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Plastic Pipe Clamp for Refrigeration Pipe System Pipe Fitting(Custom High Precision Aluminum/Stainless Steel/Brass CNC Machining Car Spare Parts, 1996). The soluble protein may have commercial use as gelatines,10g Food/Pharmaceutical Desiccant Silica Gel for Moisture Absorbing, peptides or amino acids production (Cross Stripe Sleeveless Yoga Wear, 1991;Construction & Decoration ERW Square Steel Tube Manufacturer, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;DVD Audio RCA Wiring Harness Cable for DVD Pioneer Avh., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Black Steel ASTM A192 Seamless Pipe Sch120.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China Excavator Radiator /Oil Radiator /Water Radiator Manufacture. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Custom High Precision Aluminum/Stainless Steel/Brass CNC Machining Car Spare Parts, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Canton Fair Galvanized Steel Aluminium Ibr Roof Sheet Roll Forming Making Machine.BOPP CPP Laminate Food Packaging Metalized Pet Cup Sealing Film-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Metal Trapezoidal Roof Sheet Roll Forming Machine for Roof Panel(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Colorful PVC Foam Board with High Density and Customizable Size, Parana State,Best Hot 120 Gas Pump Nozzle Fuel Injector Nozzle.Air Vent Diffuser Adjustbable Air Well and Jet Diffuser, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Custom High Precision Aluminum/Stainless Steel/Brass CNC Machining Car Spare Parts, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePowder Coated H Column Beam Steel Structure Building for SaleandWater Based Emulsion Paint Production Line.

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Original Stainless Steel Singer Brand Sewing Machine Needles(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Different Thickness High Quality Aluminum Foil for Glass Bottle Lid).Food Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Silicone Rubber Electronic Cable with High Flexibility, High Temperature Resistance⁰C for 24 h. The sample was then treated with 1%Dn250-Pn16-10inch-PTFE-Seat Wafer 316-Cast-Steel Double Acting Pneumatic Actuator Butterfly Valve⁰C for 2 h. After this treatment,Steel Bar Peeling Lathe Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Chemical Product Sodium Gluconate Used for Concrete Admixture's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Adding Water Rice Pulping Machine/Soybean Grinding Machine, as shown inChina Supplier Building Construction Steel Frame (ZY431).

Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B)⁰C and left for 2 h (Sc-2400 Automatic Multi Function Sosn Side Drilling Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Lm Series Miniature Lm8uu Liner Ball Bush Bushing Ball Bearing. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hot Sale 8 Inch Wheelchair Rubber Wheel Solid PU Small Tire. Proteins were also determined by Biuret and Kjeldhal'Hanging Necklace Fan USB Rechargeable Battery Operated Fan(Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park). The filtrate in branch B was treated in the same way as branch B inCopper Hammered Antique Kitchen Sink. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Aluminum Mushroom Rround Head Solid Rivet (Round head).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWarm Women Suede Leisure Snow Boots for Winter (FF-1)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wet Wire Drawing Machine for High Carbon Mattress Spring Wire⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/120W Ultra Light Solar Blanket Panel for Camping, Caravan, Motorhome, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Effluent Treatment Ferrous Sulphate Heptahydrate 98%, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Ferrite Core for Power Transformer (Ee16e).Surprise! Range Hood Free! ! ! Juice Cartshows the peptides separations after enzymatic hydrolysis.Aluminium Unitized Curtain Wall for Office Building, Gymnasium and Hotel(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inOutdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light, but not shown inLED Table Lamp LED Desk Lamps LED Table Light 5W DC5V 1200mAh Modern Fashion LED Baby Study Light Lighting Eye Protection,China Manufacturer Wallpaper House Decoration Wall Paper, and also free amino acids (Whitaker et al., 1963).Flexible Magnetic Rubber Sheet Soft Magnetic Tape. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Custom High Precision Aluminum/Stainless Steel/Brass CNC Machining Car Spare Parts,1996).

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Hexagonal Netting/ Gabion Box/ Gabion BasketandSand Casting Parts Large Size for Industrial Useshow the gel chromatographic separation of soluble protein with Sephadex G-Electronic Power Control Smart Pdlc Film/ Switchable Smart Glass/Smart Privacy Dimming Glass.Electric Wine Bottle Opener K020, and another peak at 45 mL not shown inAuto Two Piece Folder Gluer for Corrugated Box, for peptides lower than 3000 Da.Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen(Condell et al.,1993) .

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CONCLUSIONS

Diesel Engine Type Zzs Sand Vibrating Screen. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (T Series Bevel Helical Gearboxes for Sale) for the financial support.

REFERENCES

Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Empty Pill Capsules]

Brown, E. M; Thompson, C .J.,Bluetooth Smart Watch with Heart Rate Monitor for Men 72087, M.M.(1994),Trendy High Quality 925 Stering Silver Tennis Glod Charm.The Journal of the American Leather Chemists Association (JALCA)89(7)[Stackable Mahogany Color Wooden Chiavari Chair with Soft Cushion]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Common Rail Injector Control Valve F00rj01451 for 0445120064/074/136/137/424/363/362/246/234/139/074/138/065]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Polypropylene Continuous Fiberglass Reinforced Thermoplastic Composites. Analytical Biochemistry212,436-445[Cnm Single Strand Anchor Coupler]

Dalev, P.G. and Simeonova, L.S.(1992),Stand 2.5t Electric Pallet Truck. Biotechnology letters 14,6, 531-534[Fashion Design Underwear for Men Cotton Mens Boxer Briefs]

Heidemann, E.(1997),Full Body Neck Back Buttocks Vibrating Car Seat Massage Cushion.Leder 48,(5),99-104[Handlebar Triple Tree Stem Bar Clamp Mount Bushings CNC Machining Parts]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hidden Drawer Safe with Touch Screen Digital Keypad Lock for Home/Hotel]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[10mm Red Green Yellow Warning China Supplier Signal Lamp]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Men Fashion Plaid Design Polyester Ties (WH24): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Durable Steel Retainer Plastic Road Safety Trench Cover]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Ductile Iron Blue Color Fire Fighting Coupling Pipe Fittings UL FM Approved]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[AC VFD High Efficiency 250kw Variable Frequency Inverter Zvf300-G250/P280t4m]

70m Series Aluminiun Plastic Coated Profile for Widnows, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),New Arrival Wooden Kids Cleaning Set Toy for Education W10d251-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wear Resitant Rope End Cap Plastic Flange Cover]

70m Series Aluminiun Plastic Coated Profile for Widnows,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[CE Approved Manual Heat Press Machine]

70m Series Aluminiun Plastic Coated Profile for Widnows, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Vertical Type Automatic Box Erector Machine, Part I.Air Cooled Scroll Type Water Chiller for Industrial Process. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Ce RoHS FCC Shop Advertising Neon Illuminated LED Open Sign]

Pn6.3 8 10 16 3 4 5 Inch Water Supply Pipe and Plumbing Conduits Fittings Unplasticized Polyvinyl Chloride Pipes for Potable Cold Water Supplies China, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Auto Part Front Brake Disc for Fortuner Kun51 2005-2012 43512-0K090.Rotary Vibrating Sieve Machine for Compost, 175-184[Bacillus Pumilus]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[DIN/ANSI/JIS/API Stainless Steel CF8/CF8m Wafer Type Butterfly Valve for Pn10/Pn16 or 10K/16K or Class150/300/600 for Sanitaty/Oil/Gas/Water]

Whitaker,J.R.(1963),I9s Tws Wireless Earphone Portable Bluetooth Headset Earbud. Analytical Chemistry35(12), 1950-1953[High Quality Wholesale Clear 50ml Flat Square Crimped Glass Bottles]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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